Invitrogen

Phospho-EIF2S1 (Ser52) Monoclonal Antibody (S.674.5)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

4 Published Figures

10 References

View all (53) EIF2S1 antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

Phospho-EIF2S1 (Ser52) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 7

Phospho-EIF2S1 (Ser52) Antibody (MA5-15133) in IHC (P)

Immunohistochemical analysis of Phospho-eIF2-alpha pSer52 in paraffin-embedded human lung carcinoma using a Phospho-eIF2-alpha pSer52 monoclonal antibody (Product # MA5-15133) in the presence of control peptide (left) or Phospho-eIF2alpha (Ser52) blocking peptide (right).

Phospho-EIF2S1 (Ser52) Antibody in Immunoprecipitation (IP)

Phospho-EIF2S1 (Ser52) Antibody in Western Blot (WB)

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Product Details

MA5-15133

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:75-1:300

Immunoprecipitation (IP)

1:50

Product Specifications

Species Reactivity

Fruit fly, Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

S.674.5

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pSer52 of human eIF2alpha

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

97 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10983400

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. The polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Host Tau Genotype Specifically Designs and Regulates Tau Seeding and Spreading and Host Tau Transformation Following Intrahippocampal Injection of Identical Tau AD Inoculum.

International journal of molecular sciences

Andrés-Benito P,Carmona M,Jordán M,Fernández-Irigoyen J,Santamaría E,Del Rio JA,Ferrer I

MA5-15133 was used in Immunohistochemistry to investigate the importance of host tau in tau seeding and the molecular changes associated with the transformation of host tau into abnormal tau.

Mon Jan 10 00:00:00 EST 2022

Protective Effect of Anthocyanin-Enriched Polyphenols from Hibiscus syriacus L. (Malvaceae) against Ultraviolet B-Induced Damage.

Antioxidants (Basel, Switzerland)

Karunarathne WAHM,Molagoda IMN,Lee KT,Choi YH,Yu SM,Kang CH,Kim GY

MA5-15133 was used in Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to investigate the protective effect of AHs against UVB-induced damage.

Fri Apr 09 00:00:00 EDT 2021

Protective Effect of Anthocyanin-Enriched Polyphenols from Hibiscus syriacus L. (Malvaceae) against Ultraviolet B-Induced Damage.

Antioxidants (Basel, Switzerland)

Karunarathne WAHM,Molagoda IMN,Lee KT,Choi YH,Yu SM,Kang CH,Kim GY

MA5-15133 was used in Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to investigate the protective effect of AHs against UVB-induced damage.

Fri Apr 09 00:00:00 EDT 2021

Fisetin Protects HaCaT Human Keratinocytes from Fine Particulate Matter (PM_2.5)-Induced Oxidative Stress and Apoptosis by Inhibiting the Endoplasmic Reticulum Stress Response.

Antioxidants (Basel, Switzerland)

Molagoda IMN,Kavinda MHD,Choi YH,Lee H,Kang CH,Lee MH,Lee CM,Kim GY

MA5-15133 was used in Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to suggest that fisetin inhibits PM2.5-induced apoptosis by inhibiting the ER stress response and production of ROS.

Sat Sep 18 00:00:00 EDT 2021

IRE1A Stimulates Hepatocyte-Derived Extracellular Vesicles That Promote Inflammation in Mice With Steatohepatitis.

Gastroenterology

Dasgupta D,Nakao Y,Mauer AS,Thompson JM,Sehrawat TS,Liao CY,Krishnan A,Lucien F,Guo Q,Liu M,Xue F,Fukushima M,Katsumi T,Bansal A,Pandey MK,Maiers JL,DeGrado T,Ibrahim SH,Revzin A,Pavelko KD,Barry MA,Kaufman RJ,Malhi H

MA5-15133 was used in Western Blotting to show inflammation during steatohepatitis can be stimulated by unfolded protein response sensor IRE1A.

Thu Oct 01 00:00:00 EDT 2020

Cells

Mytych J,Sołek P,Będzińska A,Rusinek K,Warzybok A,Tabęcka-Łonczyńska A,Koziorowski M

MA5-15133 was used in Western Blotting to provide evidence of klotho involvement in the crosstalk on the line ER-Golgi, which may, in turn, affect activation of SASP.

Tue Jan 21 00:00:00 EST 2020

Extracellular and ER-stored Ca²⁺ contribute to BIRD-2-induced cell death in diffuse large B-cell lymphoma cells.

Cell death discovery

Bittremieux M,La Rovere RM,Schuermans M,Luyten T,Mikoshiba K,Vangheluwe P,Parys JB,Bultynck G

Published figure using Phospho-eIF2a (Ser51) monoclonal antibody (Product # MA5-15133) in Western Blot

Wed Sep 30 00:00:00 EDT 2020

Fatal familial insomnia: mitochondrial and protein synthesis machinery decline in the mediodorsal thalamus.

Brain pathology (Zurich, Switzerland)

Frau-Méndez MA,Fernández-Vega I,Ansoleaga B,Blanco Tech R,Carmona Tech M,Antonio Del Rio J,Zerr I,Llorens F,José Zarranz J,Ferrer I

MA515133 was used in western blot to examine the composition of mitochondrial proteins in cases of fatal familial insomnia

Sun Jan 01 00:00:00 EST 2017

RAN translation at C9orf72-associated repeat expansions is selectively enhanced by the integrated stress response.

Nature communications

Green KM,Glineburg MR,Kearse MG,Flores BN,Linsalata AE,Fedak SJ,Goldstrohm AC,Barmada SJ,Todd PK

Published figure using Phospho-eIF2a (Ser51) monoclonal antibody (Product # MA5-15133) in Western Blot

Fri Dec 08 00:00:00 EST 2017

The High-Risk Human Papillomavirus E6 Oncogene Exacerbates the Negative Effect of Tryptophan Starvation on the Development of Chlamydia trachomatis.

PloS one

Sherchand SP,Ibana JA,Zea AH,Quayle AJ,Aiyar A

MA5-15133 was used in western blot to study the effect of tryptophan starvation on chlamydial development

Wed Dec 16 00:00:00 EST 2020

VCP and ATL1 regulate endoplasmic reticulum and protein synthesis for dendritic spine formation.

Nature communications

Shih YT,Hsueh YP

MA5-15133 was used in western blot to show how ATL1 and VCP work together to regulate endoplasmic reticulum and protein synthesis for dendritic spine formation

Thu Mar 17 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: eIF-2-alpha; EIF-2A; EIF-2alpha; eIF2 alpha; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; eukaryotic translation initiation factor 2A

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Gene Aliases: 0910001O23Rik; 2410026C18Rik; 35kDa; EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A; eIF2alpha; EIF2S1

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UniProt ID: (Human) P05198, (Rat) P68101, (Mouse) Q6ZWX6

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Entrez Gene ID: (Human) 1965, (Rat) 54318, (Mouse) 13665

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Function(s)

translation initiation factor activity protein binding ribosome binding poly(A) RNA binding nucleic acid binding RNA binding translation initiation factor

Process(es)

positive regulation of defense response to virus by host translational initiation aging negative regulation of translational initiation in response to stress cellular response to amino acid starvation cellular response to heat cellular response to UV response to endoplasmic reticulum stress PERK-mediated unfolded protein response protein autophosphorylation transmembrane transport mitophagy in response to mitochondrial depolarization xenophagy positive regulation of neuron death response to manganese-induced endoplasmic reticulum stress positive regulation of type B pancreatic cell apoptotic process translation regulation of translational initiation in response to stress regulation of translation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-EIF2S1 (Ser52) Monoclonal Antibody (S.674.5)

Phospho-EIF2S1 (Ser52) Antibody (MA5-15133) in IHC (P)

Product Details

MA5-15133

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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