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Phospho-EIF6 (Ser235) Antibody (PA5-106173) in IHC (P)
Immunohistochemistry analysis of Phospho-EIF6 (Ser235) in human colorectal cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-EIF6 (Ser235) polyclonal antibody (Product # PA5-106173) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-106173
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human EIF6(Accession P56537), corresponding to amino acid residues around phosphorylated Ser235.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2817571
Product Specific Information
Antibody detects endogenous levels of EIF6 only when phosphorylated at Ser235.
Target Information
Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple transcript variants encoding two different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B(2)GCN homolog; B4 integrin interactor; CAB; eIF-6; eIF-6 {ECO:0000255|HAMAP-Rule:MF_03132}; EIF6; eukaryotic translation initiation factor 3A; Eukaryotic translation initiation factor 6; eukaryotic translation initiation factor 6 {ECO:0000255|HAMAP-Rule:MF_03132}; integrin beta 4 binding protein; p27 beta-4 integrin-binding protein; p27(BBP)
Gene Aliases: 1110004P11; AA408895; b(2)gcn; CAB; eIF-6; EIF3A; EIF6; imc-415; ITGB4BP; OK/SW-cl.27; p27(BBP); p27BBP
UniProt ID: (Human) P56537, (Rat) Q3KRD8, (Mouse) O55135
Entrez Gene ID: (Human) 3692, (Rat) 305506, (Mouse) 16418
ribosomal subunit export from nucleus
maturation of 5.8S rRNA
maturation of LSU-rRNA
regulation of glycolytic process
translational initiation
response to insulin
gene silencing by miRNA
miRNA mediated inhibition of translation
mature ribosome assembly
regulation of fatty acid biosynthetic process
regulation of megakaryocyte differentiation
positive regulation of translation
assembly of large subunit precursor of preribosome
regulation of reactive oxygen species metabolic process
ribosomal large subunit biogenesis
translation
ribosome biogenesis
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