Invitrogen

Phospho-ETS1 (Thr38) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 Published Figures

8 References

View all (37) ETS1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Phospho-ETS1 (Thr38) Antibody in Western Blot (WB)

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Phospho-ETS1 (Thr38) Antibody (44-1104G) in WB

Left panel: Extracts of Jurkat cells untreated (1) or treated with 10 µM hydrogen peroxide for 10 minutes (2-5) were resolved on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 3% milk-TBST buffer for one hour at room temperature, then incubated with the ETS1 (pT38) antibody overnight at 4°C in a 1% milk-TBST buffer, following prior incubation with: no peptide (1, 2), the non... View More

Phospho-ETS1 (Thr38) Antibody in Immunocytochemistry (ICC/IF)

Phospho-ETS1 (Thr38) Antibody in Western Blot, Immunohistochemistry (WB, IHC)

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Product Details

44-1104G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Rabbit, Rat, Xenopus

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human ETS1 that contains threonine 38.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533574

Target Information

ETS1 (c-Ets-1; v-ets erythroblastosis virus E26 oncogene homlog 1 (avian); ETS-1; EWSR2; p54), a 441 A. A protein, is a member of DNA-binding ETS protein family. It is a multifunctional protein consisting of an N-terminal pointed domain, a central transactivation domain and 2 auto inhibitory domains flanked on both sides of conserved C-terminal Ets domain. It interacts with factors including PEBP2alphaA, p300/CBP and mediates transactivation of Osteopontin, Presenilin-1, Stromelysin and Collagenase. It is activated by Ras/MAPK signaling and its expression is up-regulated in resting T-cells. Its activity is modulated by various factors including AP-1, Sp-1, c-myb and MafB. Sp100 activates ETS1 in nuclear bodies. It interacts with EAP1/Daxx and causes repression of MMP1 and BCL2 transactivation. It interacts with STAT6 and modulates Socs-1 expression induced by IL-4. It interacts with GATA3 and positively regulates Tax-1 dependent IL-5 expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Avian erythroblastosis virus E26 oncogene homolog-1 (ETS-1) plays a role in renal microvascular pathophysiology in the Dahl salt-sensitive rat.

Kidney international

Feng W,Guan Z,Xing D,Li X,Ying WZ,Remedies CE,Inscho EW,Sanders PW

44-1104G was used in Immunohistochemistry to test whether ETS-1 is involved in hypertension-induced renal microvascular pathology and autoregulatory impairment.

Sun Mar 01 00:00:00 EST 2020

Avian erythroblastosis virus E26 oncogene homolog-1 (ETS-1) plays a role in renal microvascular pathophysiology in the Dahl salt-sensitive rat.

Kidney international

Feng W,Guan Z,Xing D,Li X,Ying WZ,Remedies CE,Inscho EW,Sanders PW

44-1104G was used in Immunohistochemistry to test whether ETS-1 is involved in hypertension-induced renal microvascular pathology and autoregulatory impairment.

Sun Mar 01 00:00:00 EST 2020

Regulation of cisplatin-resistant head and neck squamous cell carcinoma by the SRC/ETS-1 signaling pathway.

BMC cancer

Yang Z,Liao J,Carter-Cooper BA,Lapidus RG,Cullen KJ,Dan H

44-1104G was used in Western Blotting to investigate the role of the ETS-1 transcription factor in Head and Neck Squamous Cell Carcinoma in multiple cisplatin-resistant HNSCC cell lines.

Wed May 22 00:00:00 EDT 2019

Transcription factor avian erythroblastosis virus E26 oncogen homolog-1 is a novel mediator of renal injury in salt-sensitive hypertension.

Hypertension (Dallas, Tex. : 1979)

Feng W,Chumley P,Prieto MC,Miyada K,Seth DM,Fatima H,Hua P,Rezonzew G,Sanders PW,Jaimes EA

Published figure using Phospho-ETS1 (Thr38) polyclonal antibody (Product # 44-1104G) in Immunofluorescence

Wed Apr 01 00:00:00 EDT 2015

Transcription factor avian erythroblastosis virus E26 oncogen homolog-1 is a novel mediator of renal injury in salt-sensitive hypertension.

Hypertension (Dallas, Tex. : 1979)

Feng W,Chumley P,Prieto MC,Miyada K,Seth DM,Fatima H,Hua P,Rezonzew G,Sanders PW,Jaimes EA

Published figure using Phospho-ETS1 (Thr38) polyclonal antibody (Product # 44-1104G) in Immunofluorescence

Wed Apr 01 00:00:00 EDT 2015

Activation of MAPK pathways due to DUSP4 loss promotes cancer stem cell-like phenotypes in basal-like breast cancer.

Cancer research

Balko JM,Schwarz LJ,Bhola NE,Kurupi R,Owens P,Miller TW,Gómez H,Cook RS,Arteaga CL

44-1104G was used in western blot to examine how DUSP4 regulates the MAP-ERK kinase and c-jun-NH2-kinase pathways in modifying cancer stem cell-like behavior.

Tue Oct 15 00:00:00 EDT 2013

Profiling of residual breast cancers after neoadjuvant chemotherapy identifies DUSP4 deficiency as a mechanism of drug resistance.

Nature medicine

Balko JM,Cook RS,Vaught DB,Kuba MG,Miller TW,Bhola NE,Sanders ME,Granja-Ingram NM,Smith JJ,Meszoely IM,Salter J,Dowsett M,Stemke-Hale K,González-Angulo AM,Mills GB,Pinto JA,Gómez HL,Arteaga CL

44-1104G was used in western blot to identify genes associated with neoadjuvant chemotherapy resistance.

Sun Jul 01 00:00:00 EDT 2012

c-Jun N-terminal kinase binding domain-dependent phosphorylation of mitogen-activated protein kinase kinase 4 and mitogen-activated protein kinase kinase 7 and balancing cross-talk between c-Jun N-terminal kinase and extracellular signal-regulated kinase pathways in cortical neurons.

Neuroscience

Repici M,Mare L,Colombo A,Ploia C,Sclip A,Bonny C,Nicod P,Salmona M,Borsello T

Tue Mar 03 00:00:00 EST 2009

Bioinformatics

Protein Aliases: Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1; avian erythroblastosis virus E26 oncogene 1; c-ets protein; c-ets-1 oncogene; c-ets-1 protein; c-ets-alpha; Ets avian erythroblastosis virus E2 oncogene homolog 1 (tumor progression locus 1); ets protein; Ets-1Delta(III-VI); FLJ10768; oncoprotein; p54; p54 transcription factor; Protein C-ets-1; TNFAIP3 interacting protein 1; Transforming protein p54/c-ets-1; Transforming protein p68/c-ets-1; tumor progression locus 1; unnamed protein product; v-ets avian erythroblastosis virus E2 oncogene homolog 1; v-ets avian erythroblastosis virus E26 oncogene homolog 1; v-ets erythroblastosis virus E26 oncogene homolog 1; virion-associated nuclear-shuttling protein

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Gene Aliases: AI196000; AI448617; c-ets-1; c-ets-beta; c-ets1; D230050P06; ETS-1; ETS-1A; ETS-1B; ETS1; Etsoncb; EWSR2; p54; TNIP1; Tpl1

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UniProt ID: (Chicken) P13474, (Human) P14921, (Rat) P41156, (Mouse) P27577

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Entrez Gene ID: (Chicken) 396235, (Human) 2113, (Rabbit) 100009373, (Rat) 24356, (Mouse) 23871

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Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding protein binding transcription factor binding histone acetyltransferase binding identical protein binding winged helix/forkhead transcription factor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-ETS1 (Thr38) Polyclonal Antibody

Phospho-ETS1 (Thr38) Antibody (44-1104G) in WB

Product Details

44-1104G

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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