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Phospho-EphA4 (Tyr779) Antibody (PA5-143695) in WB
Western blot of Phospho-EphA4 (Tyr779) in human umbilical vein endothelial cells untreated (lanes 1, 3, 5, & 7) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4, 6, & 8). The blot was probed with anti-EphA4 (N-terminal region) (lanes 1 & 2), Phospho-EphA4 (Tyr779) polyclonal antibody (Product # PA5-143695) (lanes 3 & 4), anti-EphA4 (Tyr602) (lanes 5 & 6), or anti-EphA4 (C-terminal region) (lane... View More
Product Details
PA5-143695
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
EphA4 (Tyr-779) synthetic peptide corresponds to amino acids surrounding phosphorylated tyrosine 779 in human EphA4. This sequence has significant homology to the conserved site in rat and mouse EphA4, and is conserved well in most other EphA family members, but has less conservation in EphB family members.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2942923
Product Specific Information
This antibody was cross-adsorbed to unrelated phosphotyrosine peptides before affinity purification using EphA4 (Tyr-779) peptide. The purified antibody detects a 120 kDa* band corresponding to EphA4 in western blots of HUVEC cells treated with pervanadate.
Target Information
EPHA4 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediated developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EphA4 encodes a protein that binds ephrin-A ligands.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EK8; epa4; EPH-like kinase 8; Ephrin type-A receptor 4; receptor protein-tyrosine kinase HEK8; TYRO1 protein tyrosine kinase; Tyrosine-protein kinase receptor MPK-3; Tyrosine-protein kinase receptor SEK; Tyrosine-protein kinase receptor SEK-1; Tyrosine-protein kinase TYRO1
Gene Aliases: 2900005C20Rik; AI385584; Cek8; EPHA4; HEK8; rb; RGD1560587; SEK; Sek1; TYRO1
UniProt ID: (Human) P54764, (Mouse) Q03137
Entrez Gene ID: (Human) 2043, (Rat) 316539, (Mouse) 13838
protein kinase activity
GPI-linked ephrin receptor activity
transmembrane-ephrin receptor activity
protein binding
ATP binding
PH domain binding
identical protein binding
ephrin receptor binding
DH domain binding
nucleotide binding
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
ephrin receptor activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
transmembrane signal receptor
cell adhesion
adult walking behavior
motor neuron axon guidance
glial cell migration
peptidyl-tyrosine phosphorylation
corticospinal tract morphogenesis
regulation of GTPase activity
positive regulation of JUN kinase activity
protein autophosphorylation
ephrin receptor signaling pathway
negative regulation of axon regeneration
regulation of astrocyte differentiation
regulation of axonogenesis
positive regulation of dendrite morphogenesis
regulation of dendritic spine morphogenesis
nephric duct morphogenesis
fasciculation of sensory neuron axon
fasciculation of motor neuron axon
positive regulation of Rho guanyl-nucleotide exchange factor activity
axon guidance
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
multicellular organism development
nervous system development
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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