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Phospho-EphB2 (Tyr317) Antibody (PA1-4623) in WB
Western blot of EphB2 in rat testes lysate showing specific immunolabeling of a band at ~46 kDa corresponding to Phospho-EphB2 (Tyr317) polyclonal antibody (Product # PA1-4623) in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (1,200 units for 30 min).
Product Details
PA1-4623
Product Specifications
Species Reactivity
Chicken,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr317 of chicken EPHB2 conjugated to KLH
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2095687
Product Specific Information
This antibody is predicted to react with bovine, human, mouse, Xenopus and zebrafish based on 100% sequence homology.
This antibody is specific for the ~46 Dak EphrinB protein phosphorylated at Tyr317. Immunolabeling can be blocked by lambda-phosphatase treatment.
Target Information
EPHB2 is a receptor tyrosine kinase that belongs to the ephrin receptor family. Members of the Eph family of kinases play important roles in diverse biological processes including nervous system development, angiogenesis and neural synapsis formation and maturation. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB4 binds to ephrin-B2 and plays an essential role in vascular development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cEK5; chicken embryo kinase 5; EFL6; EFNB3; EK5; embryo kinase 5 protein CEK5; EPH B2; EPH-like kinase 5; EPHB3; Ephrin B1; Ephrin B2; Ephrin B3; Ephrin B4; Ephrin type-B receptor 2; EphrinB1; EphrinB2; EphrinB3; EphrinB4; EPLG8; ETK2; HEK-6; HEK2; LERK8; MGC87492; Neural kinase; Nuk receptor tyrosine kinase; TYRO6; Tyrosine-protein kinase receptor EPH-3; Tyrosine-protein kinase receptor SEK-3
Gene Aliases: CEK5; Drt; EPHB2; Epth3; Erk; ETECK; Hek5; Nuk; Prkm5; Qek5; RGD1564232; Sek3; Tyro5
UniProt ID: (Chicken) P28693, (Mouse) Q6GTQ7
Entrez Gene ID: (Chicken) 396513, (Mouse) 13844, (Rat) 313633
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
transmembrane-ephrin receptor activity
protein binding
ATP binding
nucleotide binding
protein kinase activity
receptor activity
ephrin receptor activity
receptor binding
axon guidance receptor activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
identical protein binding
molecular_function
transmembrane signal receptor
angiogenesis
transmembrane receptor protein tyrosine kinase signaling pathway
multicellular organism development
axon guidance
axonal fasciculation
peptidyl-tyrosine phosphorylation
central nervous system projection neuron axonogenesis
spinothalamic tract morphogenesis
positive regulation of kinase activity
ephrin receptor signaling pathway
positive regulation of synapse assembly
dendritic spine development
dendritic spine morphogenesis
cell morphogenesis
urogenital system development
protein phosphorylation
nervous system development
learning
organ morphogenesis
phosphorylation
optic nerve morphogenesis
corpus callosum development
retinal ganglion cell axon guidance
inner ear morphogenesis
regulation of neuronal synaptic plasticity
positive regulation of long-term neuronal synaptic plasticity
camera-type eye morphogenesis
regulation of axonogenesis
negative regulation of axonogenesis
regulation of body fluid levels
palate development
commissural neuron axon guidance
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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