Invitrogen

Phospho-FAK (Tyr407) Polyclonal Antibody

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This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

18 Published Figures

28 References

View all (102) FAK antibodies

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Phospho-FAK (Tyr407) Antibody (44-650G)

Focal Adhesion Kinase (FAK) Protein Schematic-Focal Adhesion Kinase (FAK) is a non-receptor protein tyrosine kinase discovered as a substrate for Src and as a key element of integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion; however, dephosphor... View More

Phospho-FAK (Tyr407) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Phospho-FAK (Tyr407) Antibody in Immunocytochemistry (ICC/IF)

Phospho-FAK (Tyr407) Antibody in Immunohistochemistry (IHC)

Phospho-FAK (Tyr407) Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Phospho-FAK (Tyr407) Antibody in Western Blot (WB)

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Product Details

44-650G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

Assay-dependent

View 5 publications 5 publications

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 6 publications 6 publications

Immunoprecipitation (IP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide from human FAK containing tyrosine 407. The sequence is conserved in mouse, rat and chicken.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533708

Target Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MARK2 and MARK4 Regulate Sertoli Cell BTB Dynamics Through Microtubule and Actin Cytoskeletons.

Endocrinology

Tang EI,Cheng CY

44-650G was used in Immunohistochemistry to report the function of MARK4 and another isoform MARK2 in Sertoli cells at the BTB.

Tue Oct 11 00:00:00 EDT 2022

Endothelial neuropilin-2 influences angiogenesis by regulating actin pattern development and α5-integrin-p-FAK complex recruitment to assembling adhesion sites.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Benwell CJ,Taylor JAGE,Robinson SD

44-650G was used in Immunohistochemistry, Western Blot, Immunocytochemistry to show that neuropilin-2 (NRP2) regulates VASP-mediated control of actin pattern development to support the protrusion of dynamic lamellipodia at the leading edge of migrating cells, and also elucidate the existence of a NRP2-regulated phospho-FAK (p-FAK)-Rab11 trafficking axis with siRNA-mediated modulation.

Sun Aug 01 00:00:00 EDT 2021

Endothelial neuropilin-2 influences angiogenesis by regulating actin pattern development and α5-integrin-p-FAK complex recruitment to assembling adhesion sites.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Benwell CJ,Taylor JAGE,Robinson SD

Sun Aug 01 00:00:00 EDT 2021

Endothelial neuropilin-2 influences angiogenesis by regulating actin pattern development and α5-integrin-p-FAK complex recruitment to assembling adhesion sites.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Benwell CJ,Taylor JAGE,Robinson SD

Sun Aug 01 00:00:00 EDT 2021

Perfluorooctanesulfonate (PFOS)-induced Sertoli cell injury through a disruption of F-actin and microtubule organization is mediated by Akt1/2.

Scientific reports

Gao Y,Chen H,Xiao X,Lui WY,Lee WM,Mruk DD,Cheng CY

44-650G was used in Immunocytochemistry-immunoflourescence to investigate the effect of PFOS on Setoli cell blood-testis barrier, showing that injury occurs via Akt1/2 and disrupts F-actin and microtubule organization.

Mon Apr 24 00:00:00 EDT 2017

Perfluorooctanesulfonate (PFOS)-induced Sertoli cell injury through a disruption of F-actin and microtubule organization is mediated by Akt1/2.

Scientific reports

Gao Y,Chen H,Xiao X,Lui WY,Lee WM,Mruk DD,Cheng CY

Mon Apr 24 00:00:00 EDT 2017

Connexin 43 reboots meiosis and reseals blood-testis barrier following toxicant-mediated aspermatogenesis and barrier disruption.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Li N,Mruk DD,Mok KW,Li MW,Wong CK,Lee WM,Han D,Silvestrini B,Cheng CY

44-650G was used in immunohistochemistry - frozen section to test if overexpression of Connexin 43 in adjudin-treated rats reseals the disrupted blood-testis barrier and reinitiates spermatogenesis

Fri Apr 01 00:00:00 EDT 2016

Coordination of Actin- and Microtubule-Based Cytoskeletons Supports Transport of Spermatids and Residual Bodies/Phagosomes During Spermatogenesis in the Rat Testis.

Endocrinology

Tang EI,Lee WM,Cheng CY

44-650G was used in immunohistochemistry - frozen section to study transport of spermatids and residual bodies/phagosomes during spermatogenesis by coordination of microtuble-and actin-based cytoskeletons in rat testis

Fri Apr 01 00:00:00 EDT 2016

Simultaneous deactivation of FAK and Src improves the pathology of hypertrophic scar.

Scientific reports

Su L,Li X,Wu X,Hui B,Han S,Gao J,Li Y,Shi J,Zhu H,Zhao B,Hu D

44-650G was used in immunocytochemistry, immunohistochemistry - paraffin section, and western blot to study improvement of the pathology of hypertrophic scar by simultaneous deactivation of FAK and Src

Mon May 16 00:00:00 EDT 2016

Defining the phospho-adhesome through the phosphoproteomic analysis of integrin signalling.

Nature communications

Robertson J,Jacquemet G,Byron A,Jones MC,Warwood S,Selley JN,Knight D,Humphries JD,Humphries MJ

44-650G was used in immunocytochemistry and western blot to study phosphorylation events at sites of adhesion

Fri Feb 13 00:00:00 EST 2015

Defining the phospho-adhesome through the phosphoproteomic analysis of integrin signalling.

Nature communications

Robertson J,Jacquemet G,Byron A,Jones MC,Warwood S,Selley JN,Knight D,Humphries JD,Humphries MJ

44-650G was used in immunocytochemistry and western blot to study phosphorylation events at sites of adhesion

Fri Feb 13 00:00:00 EST 2015

Perfluorooctanesulfonate (PFOS) perturbs male rat Sertoli cell blood-testis barrier function by affecting F-actin organization via p-FAK-Tyr(407): an in vitro study.

Endocrinology

Wan HT,Mruk DD,Wong CK,Cheng CY

Published figure using Phospho-FAK (Tyr407) polyclonal antibody (Product # 44-650G) in Immunofluorescence

Wed Jan 01 00:00:00 EST 2014

Perfluorooctanesulfonate (PFOS) perturbs male rat Sertoli cell blood-testis barrier function by affecting F-actin organization via p-FAK-Tyr(407): an in vitro study.

Endocrinology

Wan HT,Mruk DD,Wong CK,Cheng CY

Published figure using Phospho-FAK (Tyr407) polyclonal antibody (Product # 44-650G) in Immunofluorescence

Wed Jan 01 00:00:00 EST 2014

Symmetric exchange of multi-protein building blocks between stationary focal adhesions and the cytosol.

eLife

Hoffmann JE,Fermin Y,Stricker RL,Ickstadt K,Zamir E

Published figure using Phospho-FAK (Tyr407) polyclonal antibody (Product # 44-650G) in Immunofluorescence

Tue Jun 03 00:00:00 EDT 2014

N-wasp is required for structural integrity of the blood-testis barrier.

PLoS genetics

Xiao X,Mruk DD,Tang EI,Massarwa R,Mok KW,Li N,Wong CK,Lee WM,Snapper SB,Shilo BZ,Schejter ED,Cheng CY

44-650G was used in immunohistochemistry - frozen section and western blot to elucidate N-wasp and its role in structural integrity of the blood-testis barrier

Sun Jun 01 00:00:00 EDT 2014

N-wasp is required for structural integrity of the blood-testis barrier.

PLoS genetics

Xiao X,Mruk DD,Tang EI,Massarwa R,Mok KW,Li N,Wong CK,Lee WM,Snapper SB,Shilo BZ,Schejter ED,Cheng CY

44-650G was used in immunohistochemistry - frozen section and western blot to elucidate N-wasp and its role in structural integrity of the blood-testis barrier

Sun Jun 01 00:00:00 EDT 2014

c-Yes regulates cell adhesion at the apical ectoplasmic specialization-blood-testis barrier axis via its effects on protein recruitment and distribution.

American journal of physiology. Endocrinology and metabolism

Xiao X,Mruk DD,Cheng CY

Published figure using Phospho-FAK (Tyr407) polyclonal antibody (Product # 44-650G) in Immunofluorescence

Tue Jan 15 00:00:00 EST 2013

Palladin is a regulator of actin filament bundles at the ectoplasmic specialization in adult rat testes.

Endocrinology

Qian X,Mruk DD,Wong EW,Lie PP,Cheng CY

Published figure using Phospho-FAK (Tyr407) polyclonal antibody (Product # 44-650G) in Western Blot

Wed May 01 00:00:00 EDT 2013

Focal adhesion kinase-Tyr407 and -Tyr397 exhibit antagonistic effects on blood-testis barrier dynamics in the rat.

Proceedings of the National Academy of Sciences of the United States of America

Lie PP,Mruk DD,Mok KW,Su L,Lee WM,Cheng CY

Published figure using Phospho-FAK (Tyr407) polyclonal antibody (Product # 44-650G) in Western Blot

Tue Jul 31 00:00:00 EDT 2012

Focal adhesion kinase-Tyr407 and -Tyr397 exhibit antagonistic effects on blood-testis barrier dynamics in the rat.

Proceedings of the National Academy of Sciences of the United States of America

Lie PP,Mruk DD,Mok KW,Su L,Lee WM,Cheng CY

Published figure using Phospho-FAK (Tyr407) polyclonal antibody (Product # 44-650G) in Western Blot

Tue Jul 31 00:00:00 EDT 2012

LCB 03-0110, a novel pan-discoidin domain receptor/c-Src family tyrosine kinase inhibitor, suppresses scar formation by inhibiting fibroblast and macrophage activation.

The Journal of pharmacology and experimental therapeutics

Sun X,Phan TN,Jung SH,Kim SY,Cho JU,Lee H,Woo SH,Park TK,Yang BS

Thu Mar 01 00:00:00 EST 2012

Tumor necrosis factor α-mediated restructuring of the Sertoli cell barrier in vitro involves matrix metalloprotease 9 (MMP9), membrane-bound intercellular adhesion molecule-1 (ICAM-1) and the actin cytoskeleton.

Spermatogenesis

Lydka M,Bilinska B,Cheng CY,Mruk DD

44-650G was used in western blot to examine the effects of TNFalpha on Sertoli cell barrier function

Mon Oct 01 00:00:00 EDT 2012

VEGF binding to NRP1 is essential for VEGF stimulation of endothelial cell migration, complex formation between NRP1 and VEGFR2, and signaling via FAK Tyr407 phosphorylation.

Molecular biology of the cell

Herzog B,Pellet-Many C,Britton G,Hartzoulakis B,Zachary IC

44-650G was used in western blot to identify residues of the NRP b domain needed for VEGF binding.

Mon Aug 01 00:00:00 EDT 2011

Cholesterol depletion induces anoikis-like apoptosis via FAK down-regulation and caveolae internalization.

The Journal of pathology

Park EK,Park MJ,Lee SH,Li YC,Kim J,Lee JS,Lee JW,Ye SK,Park JW,Kim CW,Park BK,Kim YN

44-650G was used in western blot to examine the role of the caveolae in cell survival and adhesion.

Wed Jul 01 00:00:00 EDT 2009

Analyzing FAK and Pyk2 in early integrin signaling events.

Current protocols in cell biology

Bernard-Trifilo JA,Lim ST,Hou S,Schlaepfer DD,Ilic D

Sat Apr 01 00:00:00 EST 2006

Analyzing FAK and Pyk2 in early integrin signaling events.

Current protocols in cell biology

Bernard-Trifilo JA,Lim ST,Hou S,Schlaepfer DD,Ilic D

Sat Apr 01 00:00:00 EST 2006

FAK integrates growth-factor and integrin signals to promote cell migration.

Nature cell biology

Sieg DJ,Hauck CR,Ilic D,Klingbeil CK,Schaefer E,Damsky CH,Schlaepfer DD

Mon May 01 00:00:00 EDT 2000

FAK integrates growth-factor and integrin signals to promote cell migration.

Nature cell biology

Sieg DJ,Hauck CR,Ilic D,Klingbeil CK,Schaefer E,Damsky CH,Schlaepfer DD

Mon May 01 00:00:00 EDT 2000

FAK integrates growth-factor and integrin signals to promote cell migration.

Nature cell biology

Sieg DJ,Hauck CR,Ilic D,Klingbeil CK,Schaefer E,Damsky CH,Schlaepfer DD

Mon May 01 00:00:00 EDT 2000

FAK integrates growth-factor and integrin signals to promote cell migration.

Nature cell biology

Sieg DJ,Hauck CR,Ilic D,Klingbeil CK,Schaefer E,Damsky CH,Schlaepfer DD

Mon May 01 00:00:00 EDT 2000

Bioinformatics

Protein Aliases: EC 2.7.10.2; FADK 1; FAK-related non-kinase polypeptide; Focal adhesion kinase 1; focal adhesion kinase isoform FAK-Del33; Focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; p125FAK; p41/p43FRNK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2; tyrosine kinase

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Gene Aliases: FADK; FAK; FAK1; FRNK; Kiaa4203; mKIAA4203; p125FAK; pp125FAK; PPP1R71; PTK2

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UniProt ID: (Human) Q05397, (Mouse) Q5DTH7, (Rat) O35346

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Entrez Gene ID: (Human) 5747, (Chicken) 396416, (Mouse) 14083, (Rat) 25614

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Function(s)

actin binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity signal transducer activity Ras guanyl-nucleotide exchange factor activity receptor binding protein binding ATP binding JUN kinase binding protein kinase binding SH2 domain binding protease binding calcium-dependent cysteine-type endopeptidase activity identical protein binding nucleotide binding integrin binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein complex binding phosphatidylinositol 3-kinase binding

Process(es)

Performance Guarantee

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Phospho-FAK (Tyr407) Polyclonal Antibody

Phospho-FAK (Tyr407) Antibody (44-650G)

Product Details

44-650G

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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