Invitrogen

Phospho-FAK (Tyr576) Polyclonal Antibody

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This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

12 Published Figures

24 References

View all (102) FAK antibodies

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Phospho-FAK (Tyr576) Antibody (44-652G)

Focal Adhesion Kinase (FAK) Protein Schematic-Focal Adhesion Kinase (FAK) is a non-receptor protein tyrosine kinase discovered as a substrate for Src and as a key element of integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion; however, dephosphor... View More

Phospho-FAK (Tyr576) Antibody in Immunocytochemistry (ICC/IF)

Phospho-FAK (Tyr576) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Phospho-FAK (Tyr576) Antibody in Western Blot (WB)

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Product Details

44-652G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 17 publications 17 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Rat, Xenopus

Published species

Human, Mouse, Xenopus, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human FAK that contains tyrosine 576. The sequence is conserved in mouse, rat, chicken and frog.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533709

Target Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Tumor Angiogenesis Is Differentially Regulated by Phosphorylation of Endothelial Cell Focal Adhesion Kinase Tyrosines-397 and -861.

Cancer research

Pedrosa AR,Bodrug N,Gomez-Escudero J,Carter EP,Reynolds LE,Georgiou PN,Fernandez I,Lees DM,Kostourou V,Alexopoulou AN,Batista S,Tavora B,Serrels B,Parsons M,Iskratsch T,Hodivala-Dilke KM

44-652G was used in Western Blotting to establish the differential requirements of EC-FAK-Y397 and EC-FAK-Y861-phosphorylation in the regulation of EC-signalling and tumor angiogenesis in vivo.

Sun Sep 01 00:00:00 EDT 2019

The PDGFRα-laminin B1-keratin 19 cascade drives tumor progression at the invasive front of human hepatocellular carcinoma.

Oncogene

Govaere O,Petz M,Wouters J,Vandewynckel YP,Scott EJ,Topal B,Nevens F,Verslype C,Anstee QM,Van Vlierberghe H,Mikulits W,Roskams T

Published figure using Phospho-FAK (Tyr576) polyclonal antibody (Product # 44-652G) in Western Blot

Thu Nov 23 00:00:00 EST 2017

A mutation in Nischarin causes otitis media via LIMK1 and NF-κB pathways.

PLoS genetics

Crompton M,Purnell T,Tyrer HE,Parker A,Ball G,Hardisty-Hughes RE,Gale R,Williams D,Dean CH,Simon MM,Mallon AM,Wells S,Bhutta MF,Burton MJ,Tateossian H,Brown SDM

Published figure using Phospho-FAK (Tyr576) polyclonal antibody (Product # 44-652G) in Western Blot

Tue Aug 01 00:00:00 EDT 2017

A Short Region of Connexin43 Reduces Human Glioma Stem Cell Migration, Invasion, and Survival through Src, PTEN, and FAK.

Stem cell reports

Jaraíz-Rodríguez M,Tabernero MD,González-Tablas M,Otero A,Orfao A,Medina JM,Tabernero A

44-652G was used in Western Blotting to show that a cell-penetrating peptide inhibited c-Src and focal adhesion kinase, and upregulated phosphatase and tensin homolog in glioma stem cells derived from patients.

Tue Aug 08 00:00:00 EDT 2017

Modulation of FAK and Src adhesion signaling occurs independently of adhesion complex composition.

The Journal of cell biology

Horton ER,Humphries JD,Stutchbury B,Jacquemet G,Ballestrem C,Barry ST,Humphries MJ

Published figure using Phospho-FAK (Tyr576) polyclonal antibody (Product # 44-652G) in Western Blot

Mon Feb 01 00:00:00 EST 2016

Vinculin b deficiency causes epicardial hyperplasia and coronary vessel disorganization in zebrafish.

Development (Cambridge, England)

Cheng F,Miao L,Wu Q,Gong X,Xiong J,Zhang J

44652G was used in immunohistochemistry - paraffin section to determine the function of vinculin in zebrafish epicardium and endocardium

Sat Oct 01 00:00:00 EDT 2016

Tumor endothelial cells promote metastasis and cancer stem cell-like phenotype through elevated Epiregulin in esophageal cancer.

American journal of cancer research

Sun L,Pan J,Yu L,Liu H,Shu X,Sun L,Lou J,Yang Z,Ran Y

44652G was used in western blot to investigate the molecular mechanisms by which human esophageal cancer endothelial cells contribute to esophageal carcinoma progression

Wed Mar 16 00:00:00 EDT 2022

Defining the phospho-adhesome through the phosphoproteomic analysis of integrin signalling.

Nature communications

Robertson J,Jacquemet G,Byron A,Jones MC,Warwood S,Selley JN,Knight D,Humphries JD,Humphries MJ

44-652G was used in western blot to study phosphorylation events at sites of adhesion

Fri Feb 13 00:00:00 EST 2015

Pelargonidin attenuates PDGF-BB-induced aortic smooth muscle cell proliferation and migration by direct inhibition of focal adhesion kinase.

Biochemical pharmacology

Son JE,Jeong H,Kim H,Kim YA,Lee E,Lee HJ,Lee KW

44-652G was used in western blot to study the preventive effects of pelargonidin toward atherosclerosis mechanistically.

Thu May 15 00:00:00 EDT 2014

LCB 03-0110, a novel pan-discoidin domain receptor/c-Src family tyrosine kinase inhibitor, suppresses scar formation by inhibiting fibroblast and macrophage activation.

The Journal of pharmacology and experimental therapeutics

Sun X,Phan TN,Jung SH,Kim SY,Cho JU,Lee H,Woo SH,Park TK,Yang BS

Thu Mar 01 00:00:00 EST 2012

Enhanced integrin mediated signaling and cell cycle progression on fibronectin mimetic peptide amphiphile monolayers.

Langmuir : the ACS journal of surfaces and colloids

Shroff K,Pearce TR,Kokkoli E

44-652G was used in western blot to study the binding affinity of the PR_b peptide ligand with the α(5)β() integrin.

Tue Jan 24 00:00:00 EST 2012

Enhanced integrin mediated signaling and cell cycle progression on fibronectin mimetic peptide amphiphile monolayers.

Langmuir : the ACS journal of surfaces and colloids

Shroff K,Pearce TR,Kokkoli E

44-652G was used in western blot to study the binding affinity of the PR_b peptide ligand with the α(5)β() integrin.

Tue Jan 24 00:00:00 EST 2012

Activation of endogenous FAK via expression of its amino terminal domain in Xenopus embryos.

PloS one

Petridou NI,Stylianou P,Christodoulou N,Rhoads D,Guan JL,Skourides PA

Thu Jan 10 00:00:00 EST 2013

Activation of endogenous FAK via expression of its amino terminal domain in Xenopus embryos.

PloS one

Petridou NI,Stylianou P,Christodoulou N,Rhoads D,Guan JL,Skourides PA

Thu Jan 10 00:00:00 EST 2013

Downregulation of Rap1GAP in human tumor cells alters cell/matrix and cell/cell adhesion.

Molecular and cellular biology

Tsygankova OM,Ma C,Tang W,Korch C,Feldman MD,Lv Y,Brose MS,Meinkoth JL

44-652G was used in western blot to elucidate the benefits of loss of Rap1GAP in cancer cells

Thu Jul 01 00:00:00 EDT 2010

Downregulation of Rap1GAP in human tumor cells alters cell/matrix and cell/cell adhesion.

Molecular and cellular biology

Tsygankova OM,Ma C,Tang W,Korch C,Feldman MD,Lv Y,Brose MS,Meinkoth JL

44-652G was used in western blot to elucidate the benefits of loss of Rap1GAP in cancer cells

Thu Jul 01 00:00:00 EDT 2010

Arrestin-3 is essential for the activation of Fyn by the luteinizing hormone receptor (LHR) in MA-10 cells.

Cellular signalling

Galet C,Ascoli M

44-652G was used in western blot to identify signaling molecules involve in LHR-provoked activation of Fyn

Wed Oct 01 00:00:00 EDT 2008

Alphavbeta3 integrin and cofilin modulate K1735 melanoma cell invasion.

Experimental cell research

Dang D,Bamburg JR,Ramos DM

44-652G was used in western blot to explore the relationship between beta3-integrin expression, phosphorylation of cofilin, and metalloproteinase production

Wed Feb 15 00:00:00 EST 2006

Analyzing FAK and Pyk2 in early integrin signaling events.

Current protocols in cell biology

Bernard-Trifilo JA,Lim ST,Hou S,Schlaepfer DD,Ilic D

Sat Apr 01 00:00:00 EST 2006

Analyzing FAK and Pyk2 in early integrin signaling events.

Current protocols in cell biology

Bernard-Trifilo JA,Lim ST,Hou S,Schlaepfer DD,Ilic D

Sat Apr 01 00:00:00 EST 2006

SRC catalytic but not scaffolding function is needed for integrin-regulated tyrosine phosphorylation, cell migration, and cell spreading.

Molecular and cellular biology

Cary LA,Klinghoffer RA,Sachsenmaier C,Cooper JA

Mon Apr 01 00:00:00 EST 2002

SRC catalytic but not scaffolding function is needed for integrin-regulated tyrosine phosphorylation, cell migration, and cell spreading.

Molecular and cellular biology

Cary LA,Klinghoffer RA,Sachsenmaier C,Cooper JA

Mon Apr 01 00:00:00 EST 2002

FAK integrates growth-factor and integrin signals to promote cell migration.

Nature cell biology

Sieg DJ,Hauck CR,Ilic D,Klingbeil CK,Schaefer E,Damsky CH,Schlaepfer DD

Mon May 01 00:00:00 EDT 2000

FAK integrates growth-factor and integrin signals to promote cell migration.

Nature cell biology

Sieg DJ,Hauck CR,Ilic D,Klingbeil CK,Schaefer E,Damsky CH,Schlaepfer DD

Mon May 01 00:00:00 EDT 2000

FAK integrates growth-factor and integrin signals to promote cell migration.

Nature cell biology

Sieg DJ,Hauck CR,Ilic D,Klingbeil CK,Schaefer E,Damsky CH,Schlaepfer DD

Mon May 01 00:00:00 EDT 2000

FAK integrates growth-factor and integrin signals to promote cell migration.

Nature cell biology

Sieg DJ,Hauck CR,Ilic D,Klingbeil CK,Schaefer E,Damsky CH,Schlaepfer DD

Mon May 01 00:00:00 EDT 2000

Bioinformatics

Protein Aliases: EC 2.7.10.2; FADK 1; FAK-related non-kinase polypeptide; Focal adhesion kinase 1; focal adhesion kinase isoform FAK-Del33; focal adhesion kinase pp125FAK; Focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; p125FAK; p41/p43FRNK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2; tyrosine kinase

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Gene Aliases: FADK; FAK; FAK1; FRNK; Kiaa4203; mKIAA4203; p125FAK; pp125FAK; PPP1R71; PTK2; XELAEV_18032408mg; XPFAK

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UniProt ID: (Human) Q05397, (Mouse) Q5DTH7, (Rat) O35346, (Xenopus) Q91738

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Entrez Gene ID: (Human) 5747, (Chicken) 396416, (Mouse) 14083, (Rat) 25614, (Xenopus) 399286

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Function(s)

actin binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity signal transducer activity Ras guanyl-nucleotide exchange factor activity receptor binding protein binding ATP binding JUN kinase binding protein kinase binding SH2 domain binding protease binding calcium-dependent cysteine-type endopeptidase activity identical protein binding nucleotide binding integrin binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein complex binding phosphatidylinositol 3-kinase binding

Process(es)

Performance Guarantee

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Phospho-FAK (Tyr576) Polyclonal Antibody

Phospho-FAK (Tyr576) Antibody (44-652G)

Product Details

44-652G

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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