Invitrogen

Phospho-FAK (Tyr861) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

41 Published Figures

67 References

View all (102) FAK antibodies

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Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Phospho-FAK (Tyr861) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 46

Phospho-FAK (Tyr861) Antibody (44-626G) in ICC/IF

Immunofluorescence analysis of Phospho-FAK (Tyr861) was done on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-FAK (Tyr861) Rabbit Polyclonal Antibody (Product # 44-626G) at 1:250 dilution in 1% BSA and incubated... View More

Phospho-FAK (Tyr861) Antibody in Western Blot (WB)

Phospho-FAK (Tyr861) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Phospho-FAK (Tyr861) Antibody in Western Blot, Immunocytochemistry, Immunohistochemistry (Paraffin) (WB, ICC/IF, IHC (P))

Phospho-FAK (Tyr861) Antibody in Western Blot, Immunohistochemistry (Paraffin) (WB, IHC (P))

Phospho-FAK (Tyr861) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-FAK (Tyr861) Antibody in Immunohistochemistry (IHC)

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Product Details

44-626G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 35 publications 35 publications

Immunohistochemistry (IHC)

Assay-dependent

View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

1:250

View 11 publications 11 publications

Immunoprecipitation (IP)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chicken, Human, Mouse

Published species

Chicken, Dog, Hamster, Human, Mouse, Non-human primate, Rat, Xenopus, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human FAK that contains tyrosine 861. The sequence is conserved in mouse, rat, chicken and frog.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533703

Product Specific Information

The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated FAK. The final product is generated by affinity chromatography using a FAK-derived peptide that is phosphorylated at tyrosine 861.

Primary chicken embryo fibroblasts (CEF) expressing FAK protein and plated on fibronectin, or A459 whole cell lysate was used as positive control.

The target antigen, in western blotting, has an apparent MW of 125 kDa.

Target Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

BCL2L11 Induction Mediates Sensitivity to Src and MEK1/2 Inhibition in Thyroid Cancer.

Cancers

Rose MM,Espinoza VL,Hoff KJ,Pike LA,Sharma V,Hofmann MC,Tan AC,Pozdeyev N,Schweppe RE

44-626G was used in Western Blotting to provide evidence that combined Src and MEK1/2 inhibition is a promising therapeutic option for patients with advanced thyroid cancer and identifies BIM induction as a potential biomarker of response.

Fri Jan 06 00:00:00 EST 2023

Noncanonical Wnt/Ror2 signaling regulates cell-matrix adhesion to prompt directional tumor cell invasion in breast cancer.

Molecular biology of the cell

Si H,Zhao N,Pedroza A,Zaske AM,Rosen JM,Creighton CJ,Roarty K

Published figure using Phospho-FAK (Tyr861) polyclonal antibody (Product # 44-626G) in Immunohistochemistry (Paraffin)

Thu Sep 15 00:00:00 EDT 2022

Noncanonical Wnt/Ror2 signaling regulates cell-matrix adhesion to prompt directional tumor cell invasion in breast cancer.

Molecular biology of the cell

Si H,Zhao N,Pedroza A,Zaske AM,Rosen JM,Creighton CJ,Roarty K

Published figure using Phospho-FAK (Tyr861) polyclonal antibody (Product # 44-626G) in Immunohistochemistry (Paraffin)

Thu Sep 15 00:00:00 EDT 2022

Noncanonical Wnt/Ror2 signaling regulates cell-matrix adhesion to prompt directional tumor cell invasion in breast cancer.

Molecular biology of the cell

Si H,Zhao N,Pedroza A,Zaske AM,Rosen JM,Creighton CJ,Roarty K

Published figure using Phospho-FAK (Tyr861) polyclonal antibody (Product # 44-626G) in Immunohistochemistry (Paraffin)

Thu Sep 15 00:00:00 EDT 2022

Noncanonical Wnt/Ror2 signaling regulates cell-matrix adhesion to prompt directional tumor cell invasion in breast cancer.

Molecular biology of the cell

Si H,Zhao N,Pedroza A,Zaske AM,Rosen JM,Creighton CJ,Roarty K

Published figure using Phospho-FAK (Tyr861) polyclonal antibody (Product # 44-626G) in Immunohistochemistry (Paraffin)

Thu Sep 15 00:00:00 EDT 2022

Slit-Robo signalling establishes a Sphingosine-1-phosphate gradient to polarise fin mesenchyme.

EMBO reports

Mahabaleshwar H,Asharani PV,Loo TY,Koh SY,Pitman MR,Kwok S,Ma J,Hu B,Lin F,Li Lok X,Pitson SM,Saunders TE,Carney TJ

44-626G was used in Immunohistochemistry-immunofluorescence to demonstrate the coordination of the Slit-Robo and S1P signalling pathways in fin fold morphogenesis.

Wed Aug 03 00:00:00 EDT 2022

Increased Stiffness Downregulates Focal Adhesion Kinase Expression in Pancreatic Cancer Cells Cultured in 3D Self-Assembling Peptide Scaffolds.

Biomedicines

Betriu N,Andreeva A,Alonso A,Semino CE

44-626G was used in Immunocytochemistry-immunofluorescence to highlight how cell response to increased matrix stiffness greatly depends on the nature of the matrix used for 3D culture.

Fri Jul 29 00:00:00 EDT 2022

Effect of Mechanical Loading of Senescent Myoblasts on Their Myogenic Lineage Progression and Survival.

Cells

Moustogiannis A,Philippou A,Zevolis E,Taso OS,Giannopoulos A,Chatzigeorgiou A,Koutsilieris M

44-626G was used in Western Blotting to examine the effect of mechanical loading of aged, proliferating, and differentiated myoblasts on the gene expression and signaling responses associated with their myogenic lineage progression and survival.

Fri Dec 09 00:00:00 EST 2022

Phosphorylation of pericyte FAK-Y861 affects tumour cell apoptosis and tumour blood vessel regression.

Angiogenesis

Lees DM,Reynolds LE,Pedrosa AR,Roy-Luzarraga M,Hodivala-Dilke KM

44-626G was used in Western Blot to identify the role of pericyte FAK-Y861 in the regulation of tumour vessel regression and tumour growth control and that non-phosphorylatable FAK-Y861F in pericytes reduces tumour growth and blood vessel density.

Sun Aug 01 00:00:00 EDT 2021

A Cas-BCAR3 co-regulatory circuit controls lamellipodia dynamics.

eLife

Steenkiste EM,Berndt JD,Pilling C,Simpkins C,Cooper JA

44-626G was used in Immunocytochemistry to find that BCAR3 localization to integrin adhesions requires Cas.

Fri Jun 25 00:00:00 EDT 2021

Neural crest cells bulldoze through the microenvironment using Aquaporin 1 to stabilize filopodia.

Development (Cambridge, England)

McLennan R,McKinney MC,Teddy JM,Morrison JA,Kasemeier-Kulesa JC,Ridenour DA,Manthe CA,Giniunaite R,Robinson M,Baker RE,Maini PK,Kulesa PM

44-626G was used in Immunohistochemistry-wholemount to demonstrate that neural crest cells express AQP-1 mRNA and protein and investigate the function of AQP-1 signaling.

Thu Jan 09 00:00:00 EST 2020

A combined FAK, c-MET, and MST1R three-protein panel risk-stratifies colorectal cancer patients.

Translational oncology

Yoon JY,Wang JY,Roehrl MHA

44-626G was used in Immunohistochemistry (Paraffin) to examine the protein expression of FAK, c-MET, and MST1R levels in a well-annotated cohort of 330 colorectal cancer patients.

Sun Nov 01 00:00:00 EDT 2020

A window-of-opportunity trial of the CXCR1/2 inhibitor reparixin in operable HER-2-negative breast cancer.

Breast cancer research : BCR

Goldstein LJ,Perez RP,Yardley D,Han LK,Reuben JM,Gao H,McCanna S,Butler B,Ruffini PA,Liu Y,Rosato RR,Chang JC

44-626G was used in Immunohistochemistry to investigate the effects of reparixin, an allosteric inhibitor of CXCR1, on cancer stem cells (CSCs) in HER-2-negative operable breast cancer patients.

Fri Jan 10 00:00:00 EST 2020

Resistance to Src inhibition alters the BRAF-mutant tumor secretome to promote an invasive phenotype and therapeutic escape through a FAK>p130Cas>c-Jun signaling axis.

Oncogene

Kessler BE,Mishall KM,Kellett MD,Clark EG,Pugazhenthi U,Pozdeyev N,Kim J,Tan AC,Schweppe RE

44-626G was used in Western Blotting to study the mechanisms of resistance to Src inhibitor dasatinib to identify target pathways.

Mon Apr 01 00:00:00 EDT 2019

Lack of Myosin X Enhances Osteoclastogenesis and Increases Cell Surface Unc5b in Osteoclast-Lineage Cells.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Wang B,Pan JX,Yu H,Xiong L,Zhao K,Xiong S,Guo JP,Lin S,Sun D,Zhao L,Guo H,Mei L,Xiong WC

44-626G was used in Western Blotting to study the effects of Myo10 loss of function on osteoporotic regulation in mice models.

Wed May 01 00:00:00 EDT 2019

The SRC Inhibitor Dasatinib Induces Stem Cell-Like Properties in Head and Neck Cancer Cells that are Effectively Counteracted by the Mithralog EC-8042.

Journal of clinical medicine

Hermida-Prado F,Villaronga MÁ,Granda-Díaz R,Del-Río-Ibisate N,Santos L,Hermosilla MA,Oro P,Allonca E,Agorreta J,Garmendia I,Tornín J,Perez-Escuredo J,Fuente R,Montuenga LM,Morís F,Rodrigo JP,Rodríguez R,García-Pedrero JM

44-626G was used in Immunohistochemistry to unveil undesirable stem cell-promoting functions by dasatinib and saracatinib that could explain the lack of clinical efficacy of both drugs in head and neck squamaous cell carcinoma (HNSCC) patients.

Fri Aug 02 00:00:00 EDT 2019

Collagen abundance controls melanoma phenotypes through lineage-specific microenvironment sensing.

Oncogene

Miskolczi Z,Smith MP,Rowling EJ,Ferguson J,Barriuso J,Wellbrock C

Published figure using Phospho-FAK (Tyr861) polyclonal antibody (Product # 44-626G) in Western Blot

Fri Jun 01 00:00:00 EDT 2018

Src-mediated regulation of the PI3K pathway in advanced papillary and anaplastic thyroid cancer.

Oncogenesis

Beadnell TC,Nassar KW,Rose MM,Clark EG,Danysh BP,Hofmann MC,Pozdeyev N,Schweppe RE

44-626G was used in Western Blotting to show combined inhibition of Src and MAPK pathways may improve survival of advanced thyroid cancer patients with BRAF and RAS mutations.

Wed Feb 28 00:00:00 EST 2018

Treatment with Src inhibitor Dasatinib results in elevated metastatic potential in the 4T1 murine mammary carcinoma model.

Tumor & microenvironment

Hughes VS,Siemann DW

44-626G was used in Western Blotting to examine the effect of Src inhibition on the metastatic potential of the 4T1 murine mammary carcinoma.

Thu Mar 17 00:00:00 EDT 2022

AKT-mediated stabilization of histone methyltransferase WHSC1 promotes prostate cancer metastasis.

The Journal of clinical investigation

Li N,Xue W,Yuan H,Dong B,Ding Y,Liu Y,Jiang M,Kan S,Sun T,Ren J,Pan Q,Li X,Zhang P,Hu G,Wang Y,Wang X,Li Q,Qin J

44626G was used in immunocytochemistry to demonstrate that WHSC1 drives indolent PTEN-null tumors to become metastatic prostate cancer

Mon Apr 03 00:00:00 EDT 2017

Sustained activation of the AKT/mTOR and MAP kinase pathways mediate resistance to the Src inhibitor, dasatinib, in thyroid cancer.

Oncotarget

Mishall KM,Beadnell TC,Kuenzi BM,Klimczak DM,Superti-Furga G,Rix U,Schweppe RE

Published figure using Phospho-FAK (Tyr861) polyclonal antibody (Product # 44-626G) in Western Blot

Tue Nov 28 00:00:00 EST 2017

The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes.

eLife

Benito-Jardón M,Klapproth S,Gimeno-LLuch I,Petzold T,Bharadwaj M,Müller DJ,Zuchtriegel G,Reichel CA,Costell M

44626G was used in western blot to find that mice with a dysfunctional fibronectin-synergy motif suffer from surprisingly mild platelet adhesion and bleeding defects due to delayed thrombus formation after vessel injury

Mon Jan 16 00:00:00 EST 2017

Imaging collective cell migration and hair cell regeneration in the sensory lateral line.

Methods in cell biology

Venero Galanternik M,Navajas Acedo J,Romero-Carvajal A,Piotrowski T

44-626G was used in immunocytochemistry to study a sensory lateral line for hair cell regeneration and collective cell migration imaging

Mon Aug 14 00:00:00 EDT 2017

Angiopoietin 2 signaling plays a critical role in neural crest cell migration.

BMC biology

McKinney MC,McLennan R,Kulesa PM

Published figure using Phospho-FAK (Tyr861) polyclonal antibody (Product # 44-626G) in Immunofluorescence

Thu Dec 15 00:00:00 EST 2016

The anti-tumor NC1 domain of collagen XIX inhibits the FAK/ PI3K/Akt/mTOR signaling pathway through αvβ3 integrin interaction.

Oncotarget

Oudart JB,Doué M,Vautrin A,Brassart B,Sellier C,Dupont-Deshorgue A,Monboisse JC,Maquart FX,Brassart-Pasco S,Ramont L

44-626G was used in western blot to study inhibition of the FAK/PI3K/Akt/mTOR signaling pathway through av-beta-3 integrin interaction by the anti-tumor NC1 domain of collagen XIX

Tue Jan 12 00:00:00 EST 2016

The microRNA-200/Zeb1 axis regulates ECM-dependent β1-integrin/FAK signaling, cancer cell invasion and metastasis through CRKL.

Scientific reports

Ungewiss C,Rizvi ZH,Roybal JD,Peng DH,Gold KA,Shin DH,Creighton CJ,Gibbons DL

Published figure using Phospho-FAK (Tyr861) polyclonal antibody (Product # 44-626G) in Immunofluorescence

Tue Jan 05 00:00:00 EST 2016

The microRNA-200/Zeb1 axis regulates ECM-dependent β1-integrin/FAK signaling, cancer cell invasion and metastasis through CRKL.

Scientific reports

Ungewiss C,Rizvi ZH,Roybal JD,Peng DH,Gold KA,Shin DH,Creighton CJ,Gibbons DL

Published figure using Phospho-FAK (Tyr861) polyclonal antibody (Product # 44-626G) in Immunofluorescence

Tue Jan 05 00:00:00 EST 2016

Src Family Kinases Modulate the Loss of Endothelial Barrier Function in Response to TNF-α: Crosstalk with p38 Signaling.

PloS one

Adam AP,Lowery AM,Martino N,Alsaffar H,Vincent PA

44-626G was used in western blot investigate the effects of TNF treatment and SFK activation using human dermal microvascular endothelial cells

Wed Aug 02 00:00:00 EDT 2017

A Monoclonal Antibody to ADAM17 Inhibits Tumor Growth by Inhibiting EGFR and Non-EGFR-Mediated Pathways.

Molecular cancer therapeutics

Rios-Doria J,Sabol D,Chesebrough J,Stewart D,Xu L,Tammali R,Cheng L,Du Q,Schifferli K,Rothstein R,Leow CC,Heidbrink-Thompson J,Jin X,Gao C,Friedman J,Wilkinson B,Damschroder M,Pierce AJ,Hollingsworth RE,Tice DA,Michelotti EF

44-626G was used in western blot to study the effect of using an ADAM17 inhibitory antibody, MEDI3622, in several EGFR-dependent tumor models

Wed Jul 01 00:00:00 EDT 2015

Anti-metastatic action of FAK inhibitor OXA-11 in combination with VEGFR-2 signaling blockade in pancreatic neuroendocrine tumors.

Clinical & experimental metastasis

Moen I,Gebre M,Alonso-Camino V,Chen D,Epstein D,McDonald DM

Published figure using Phospho-FAK (Tyr861) polyclonal antibody (Product # 44-626G) in Immunofluorescence

Tue Dec 01 00:00:00 EST 2015

Influence of extracellular matrix components on the expression of integrins and regeneration of adult retinal ganglion cells.

PloS one

Vecino E,Heller JP,Veiga-Crespo P,Martin KR,Fawcett JW

44-626G was used in immunocytochemistry to identify factors that contribute to retinal ganglion cell survival and regeneration

Mon Apr 25 00:00:00 EDT 2016

Src inhibitors in suppression of papillary thyroid carcinoma growth.

Head & neck

Henderson YC,Toro-Serra R,Chen Y,Ryu J,Frederick MJ,Zhou G,Gallick GE,Lai SY,Clayman GL

44-626G was used in western blot to study BRAF mutations and RET/PTC rearrangements in papillary thyroid carcinomas

Sat Mar 01 00:00:00 EST 2014

FAK is required for tension-dependent organization of collective cell movements in Xenopus mesendoderm.

Developmental biology

Bjerke MA,Dzamba BJ,Wang C,DeSimone DW

44-626G was used in western blot to examine the relation between focal adhesion kinase and Xenopus mesendoderm cells.

Wed Oct 15 00:00:00 EDT 2014

Thrombospondin-4 controls matrix assembly during development and repair of myotendinous junctions.

eLife

Subramanian A,Schilling TF

Published figure using Phospho-FAK (Tyr861) polyclonal antibody (Product # 44-626G) in Immunofluorescence

Wed Jun 18 00:00:00 EDT 2014

Calcium-dependent FAK/CREB/TNNC1 signalling mediates the effect of stromal MFAP5 on ovarian cancer metastatic potential.

Nature communications

Leung CS,Yeung TL,Yip KP,Pradeep S,Balasubramanian L,Liu J,Wong KK,Mangala LS,Armaiz-Pena GN,Lopez-Berestein G,Sood AK,Birrer MJ,Mok SC

44-626G was used in western blot to identify stromal microfibrillar-associated protein 5 as a prognostic marker for poor survival of ovarian cancer

Fri Oct 03 00:00:00 EDT 2014

The effects of neuregulin on cardiac Myosin light chain kinase gene-ablated hearts.

PloS one

Chang AN,Huang J,Battiprolu PK,Hill JA,Kamm KE,Stull JT

Published figure using Phospho-FAK (Tyr861) polyclonal antibody (Product # 44-626G) in Western Blot

Thu Jan 16 00:00:00 EST 2014

FAK-heterozygous mice display enhanced tumour angiogenesis.

Nature communications

Kostourou V,Lechertier T,Reynolds LE,Lees DM,Baker M,Jones DT,Tavora B,Ramjaun AR,Birdsey GM,Robinson SD,Parsons M,Randi AM,Hart IR,Hodivala-Dilke K

44-626G was used in Immunocytochemistry-immunoflourescence to highlight a potential role for focal adhesion kinase (FAK) as a nonlinear, dose-dependent regulator of angiogenesis where heterozygous levels of FAK enhance angiogenesis.

Mon Dec 30 00:00:00 EST 2013

LCB 03-0110, a novel pan-discoidin domain receptor/c-Src family tyrosine kinase inhibitor, suppresses scar formation by inhibiting fibroblast and macrophage activation.

The Journal of pharmacology and experimental therapeutics

Sun X,Phan TN,Jung SH,Kim SY,Cho JU,Lee H,Woo SH,Park TK,Yang BS

Thu Mar 01 00:00:00 EST 2012

A randomized phase II study of cilengitide (EMD 121974) in patients with metastatic melanoma.

Melanoma research

Kim KB,Prieto V,Joseph RW,Diwan AH,Gallick GE,Papadopoulos NE,Bedikian AY,Camacho LH,Hwu P,Ng CS,Wei W,Johnson MM,Wittemer SM,Vardeleon A,Reckeweg A,Colevas AD

44-626G was used in immunohistochemistry - paraffin section to report the results of a randomized phase II trial in patients with metastatic melanoma to evaluate the clinical efficacy of cilengitide.

Wed Aug 01 00:00:00 EDT 2012

Activation of endogenous FAK via expression of its amino terminal domain in Xenopus embryos.

PloS one

Petridou NI,Stylianou P,Christodoulou N,Rhoads D,Guan JL,Skourides PA

Thu Jan 10 00:00:00 EST 2013

Activation of endogenous FAK via expression of its amino terminal domain in Xenopus embryos.

PloS one

Petridou NI,Stylianou P,Christodoulou N,Rhoads D,Guan JL,Skourides PA

Thu Jan 10 00:00:00 EST 2013

Phase I trial of a selective c-MET inhibitor ARQ 197 incorporating proof of mechanism pharmacodynamic studies.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology

Yap TA,Olmos D,Brunetto AT,Tunariu N,Barriuso J,Riisnaes R,Pope L,Clark J,Futreal A,Germuska M,Collins D,deSouza NM,Leach MO,Savage RE,Waghorne C,Chai F,Garmey E,Schwartz B,Kaye SB,de Bono JS

44-626G was used in immunohistochemistry to report the results of a phase I trial of ARQ 197.

Fri Apr 01 00:00:00 EDT 2011

PH006, a novel and selective Src kinase inhibitor, suppresses human breast cancer growth and metastasis in vitro and in vivo.

Breast cancer research and treatment

Ma JG,Huang H,Chen SM,Chen Y,Xin XL,Lin LP,Ding J,Liu H,Meng LH

44-626G was used in western blot to identify the Src kinase inhibitor, PH006, and explore its activity and mechanism of action in human breast cancer.

Tue Nov 01 00:00:00 EDT 2011

Nuclear and cytoplasmic LIMK1 enhances human breast cancer progression.

Molecular cancer

McConnell BV,Koto K,Gutierrez-Hartmann A

44-626G was used in immunocytochemistry and western blot to elucidate the role of nuclear vs cytoplasmic LIMK in the tumorigenic process.

Sat Jun 18 00:00:00 EDT 2011

Nuclear and cytoplasmic LIMK1 enhances human breast cancer progression.

Molecular cancer

McConnell BV,Koto K,Gutierrez-Hartmann A

44-626G was used in immunocytochemistry and western blot to elucidate the role of nuclear vs cytoplasmic LIMK in the tumorigenic process.

Sat Jun 18 00:00:00 EDT 2011

Nuclear and cytoplasmic LIMK1 enhances human breast cancer progression.

Molecular cancer

McConnell BV,Koto K,Gutierrez-Hartmann A

44-626G was used in immunocytochemistry and western blot to elucidate the role of nuclear vs cytoplasmic LIMK in the tumorigenic process.

Sat Jun 18 00:00:00 EDT 2011

Cellular settings mediating Src Substrate switching between focal adhesion kinase tyrosine 861 and CUB-domain-containing protein 1 (CDCP1) tyrosine 734.

The Journal of biological chemistry

Wortmann A,He Y,Christensen ME,Linn M,Lumley JW,Pollock PM,Waterhouse NJ,Hooper JD

44-626G was used in western blot to investigate SFK-mediated phosphorylation of CDCP1.

Fri Dec 09 00:00:00 EST 2011

Cellular settings mediating Src Substrate switching between focal adhesion kinase tyrosine 861 and CUB-domain-containing protein 1 (CDCP1) tyrosine 734.

The Journal of biological chemistry

Wortmann A,He Y,Christensen ME,Linn M,Lumley JW,Pollock PM,Waterhouse NJ,Hooper JD

44-626G was used in western blot to investigate SFK-mediated phosphorylation of CDCP1.

Fri Dec 09 00:00:00 EST 2011

Cellular settings mediating Src Substrate switching between focal adhesion kinase tyrosine 861 and CUB-domain-containing protein 1 (CDCP1) tyrosine 734.

The Journal of biological chemistry

Wortmann A,He Y,Christensen ME,Linn M,Lumley JW,Pollock PM,Waterhouse NJ,Hooper JD

44-626G was used in western blot to investigate SFK-mediated phosphorylation of CDCP1.

Fri Dec 09 00:00:00 EST 2011

The simian virus 40 large tumor antigen activates cSrc and requires cSrc for full neoplastic transformation.

Anticancer research

Arulanandam R,Geletu M,Raptis L

44-626G was used in western blot to explore the role of cSrc in neoplastic transformation by the large tumor antigen of simian virus 40

Fri Jan 01 00:00:00 EST 2010

Increased levels of active c-Src distinguish invasive from in situ lobular lesions.

Breast cancer research : BCR

Zou D,Yoon HS,Anjomshoaa A,Perez D,Fukuzawa R,Guilford P,Humar B

Wed Feb 24 00:00:00 EST 2010

Inhibition of Src with AZD0530 reveals the Src-Focal Adhesion kinase complex as a novel therapeutic target in papillary and anaplastic thyroid cancer.

The Journal of clinical endocrinology and metabolism

Schweppe RE,Kerege AA,French JD,Sharma V,Grzywa RL,Haugen BR

44-626G was used in immunohistochemistry - paraffin section and western blot to determine the role of the Src-FAK complex in papillary and anaplastic thyroid cancer

Mon Jun 01 00:00:00 EDT 2009

Inhibition of Src with AZD0530 reveals the Src-Focal Adhesion kinase complex as a novel therapeutic target in papillary and anaplastic thyroid cancer.

The Journal of clinical endocrinology and metabolism

Schweppe RE,Kerege AA,French JD,Sharma V,Grzywa RL,Haugen BR

44-626G was used in immunohistochemistry - paraffin section and western blot to determine the role of the Src-FAK complex in papillary and anaplastic thyroid cancer

Mon Jun 01 00:00:00 EDT 2009

Inhibition of Src with AZD0530 reveals the Src-Focal Adhesion kinase complex as a novel therapeutic target in papillary and anaplastic thyroid cancer.

The Journal of clinical endocrinology and metabolism

Schweppe RE,Kerege AA,French JD,Sharma V,Grzywa RL,Haugen BR

44-626G was used in immunohistochemistry - paraffin section and western blot to determine the role of the Src-FAK complex in papillary and anaplastic thyroid cancer

Mon Jun 01 00:00:00 EDT 2009

Src inhibitors in early breast cancer: a methodology, feasibility and variability study.

Breast cancer research and treatment

Jones RJ,Young O,Renshaw L,Jacobs V,Fennell M,Marshall A,Green TP,Elvin P,Womack C,Clack G,Dixon JM

Sun Mar 01 00:00:00 EST 2009

Src inhibitors in early breast cancer: a methodology, feasibility and variability study.

Breast cancer research and treatment

Jones RJ,Young O,Renshaw L,Jacobs V,Fennell M,Marshall A,Green TP,Elvin P,Womack C,Clack G,Dixon JM

Sun Mar 01 00:00:00 EST 2009

Postischemic gene transfer of soluble Flt-1 protects against brain ischemia with marked attenuation of blood-brain barrier permeability.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Kumai Y,Ooboshi H,Ibayashi S,Ishikawa E,Sugimori H,Kamouchi M,Kitazono T,Egashira K,Iida M

Fri Jun 01 00:00:00 EDT 2007

Development of the post-natal growth plate requires intraflagellar transport proteins.

Developmental biology

Song B,Haycraft CJ,Seo HS,Yoder BK,Serra R

44-626G was used in immunohistochemistry - paraffin section test if intraflagellar transport has a role in post-natal skeletal development

Tue May 01 00:00:00 EDT 2007

Analyzing FAK and Pyk2 in early integrin signaling events.

Current protocols in cell biology

Bernard-Trifilo JA,Lim ST,Hou S,Schlaepfer DD,Ilic D

Sat Apr 01 00:00:00 EST 2006

Analyzing FAK and Pyk2 in early integrin signaling events.

Current protocols in cell biology

Bernard-Trifilo JA,Lim ST,Hou S,Schlaepfer DD,Ilic D

Sat Apr 01 00:00:00 EST 2006

Crk-associated substrate tyrosine phosphorylation sites are critical for invasion and metastasis of SRC-transformed cells.

Molecular cancer research : MCR

Brábek J,Constancio SS,Siesser PF,Shin NY,Pozzi A,Hanks SK

Wed Jun 01 00:00:00 EDT 2005

Stat3 is required for full neoplastic transformation by the Simian Virus 40 large tumor antigen.

Molecular biology of the cell

Vultur A,Arulanandam R,Turkson J,Niu G,Jove R,Raptis L

Published figure using Phospho-FAK (Tyr861) polyclonal antibody (Product # 44-626G) in Western Blot

Mon Aug 01 00:00:00 EDT 2005

Stat3 is required for full neoplastic transformation by the Simian Virus 40 large tumor antigen.

Molecular biology of the cell

Vultur A,Arulanandam R,Turkson J,Niu G,Jove R,Raptis L

Published figure using Phospho-FAK (Tyr861) polyclonal antibody (Product # 44-626G) in Western Blot

Mon Aug 01 00:00:00 EDT 2005

Tyrosine phosphorylation of VE-cadherin prevents binding of p120- and beta-catenin and maintains the cellular mesenchymal state.

The Journal of biological chemistry

Potter MD,Barbero S,Cheresh DA

Fri Sep 09 00:00:00 EDT 2005

Tyrosine phosphorylation of VE-cadherin prevents binding of p120- and beta-catenin and maintains the cellular mesenchymal state.

The Journal of biological chemistry

Potter MD,Barbero S,Cheresh DA

Fri Sep 09 00:00:00 EDT 2005

A selective small molecule inhibitor of c-Met kinase inhibits c-Met-dependent phenotypes in vitro and exhibits cytoreductive antitumor activity in vivo.

Cancer research

Christensen JG,Schreck R,Burrows J,Kuruganti P,Chan E,Le P,Chen J,Wang X,Ruslim L,Blake R,Lipson KE,Ramphal J,Do S,Cui JJ,Cherrington JM,Mendel DB

Sat Nov 01 00:00:00 EST 2003

A selective small molecule inhibitor of c-Met kinase inhibits c-Met-dependent phenotypes in vitro and exhibits cytoreductive antitumor activity in vivo.

Cancer research

Christensen JG,Schreck R,Burrows J,Kuruganti P,Chan E,Le P,Chen J,Wang X,Ruslim L,Blake R,Lipson KE,Ramphal J,Do S,Cui JJ,Cherrington JM,Mendel DB

Sat Nov 01 00:00:00 EST 2003

A selective small molecule inhibitor of c-Met kinase inhibits c-Met-dependent phenotypes in vitro and exhibits cytoreductive antitumor activity in vivo.

Cancer research

Christensen JG,Schreck R,Burrows J,Kuruganti P,Chan E,Le P,Chen J,Wang X,Ruslim L,Blake R,Lipson KE,Ramphal J,Do S,Cui JJ,Cherrington JM,Mendel DB

Sat Nov 01 00:00:00 EST 2003

High RhoA activity maintains the undifferentiated mesenchymal cell phenotype, whereas RhoA down-regulation by laminin-2 induces smooth muscle myogenesis.

The Journal of cell biology

Beqaj S,Jakkaraju S,Mattingly RR,Pan D,Schuger L

Mon Mar 04 00:00:00 EST 2002

Regulation of the expression of c-Myc by beta1 integrins in epithelial cells.

Oncogene

Benaud CM,Dickson RB

Thu Feb 08 00:00:00 EST 2001

FAK integrates growth-factor and integrin signals to promote cell migration.

Nature cell biology

Sieg DJ,Hauck CR,Ilic D,Klingbeil CK,Schaefer E,Damsky CH,Schlaepfer DD

Mon May 01 00:00:00 EDT 2000

FAK integrates growth-factor and integrin signals to promote cell migration.

Nature cell biology

Sieg DJ,Hauck CR,Ilic D,Klingbeil CK,Schaefer E,Damsky CH,Schlaepfer DD

Mon May 01 00:00:00 EDT 2000

FAK integrates growth-factor and integrin signals to promote cell migration.

Nature cell biology

Sieg DJ,Hauck CR,Ilic D,Klingbeil CK,Schaefer E,Damsky CH,Schlaepfer DD

Mon May 01 00:00:00 EDT 2000

FAK integrates growth-factor and integrin signals to promote cell migration.

Nature cell biology

Sieg DJ,Hauck CR,Ilic D,Klingbeil CK,Schaefer E,Damsky CH,Schlaepfer DD

Mon May 01 00:00:00 EDT 2000

Bioinformatics

Protein Aliases: EC 2.7.10.2; FADK 1; FAK-related non-kinase polypeptide; Focal adhesion kinase 1; focal adhesion kinase isoform FAK-Del33; Focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; p125FAK; p41/p43FRNK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2; tyrosine kinase

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Gene Aliases: FADK; FAK; FAK1; FRNK; Kiaa4203; mKIAA4203; p125FAK; pp125FAK; PPP1R71; PTK2

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UniProt ID: (Human) Q05397, (Mouse) Q5DTH7

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Entrez Gene ID: (Human) 5747, (Chicken) 396416, (Mouse) 14083

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Function(s)

actin binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity signal transducer activity Ras guanyl-nucleotide exchange factor activity receptor binding protein binding ATP binding JUN kinase binding protein kinase binding SH2 domain binding protease binding calcium-dependent cysteine-type endopeptidase activity identical protein binding nucleotide binding integrin binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein complex binding phosphatidylinositol 3-kinase binding

Process(es)

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Phospho-FAK (Tyr861) Polyclonal Antibody

Phospho-FAK (Tyr861) Antibody (44-626G) in ICC/IF

Product Details

44-626G

Western Blot (WB)

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Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

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