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Phospho-GSK3 alpha (Ser21) Antibody (PA5-121291) in WB
Western blot analysis of Phospho-GSK3 alpha (Ser21) in extracts of 293 and HeLa cells. Samples were incubated with Phospho-GSK3 alpha (Ser21) Polyclonal antibody (Product # PA5-121291) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. 293 cells were treated by Insulin (100 nM) for 10 minutes after serum-starvation overnight. HeLa cells were... View More
Product Details
PA5-121291
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic phosphorylated peptide around S21 of human GSK3alpha (NP_0639372)
Conjugate
Form
Liquid
Concentration
1.91 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914863
Product Specific Information
Positive test controls include: 293, HeLa.
Immunogen sequence: TSSFA
Target Information
Glycogen synthase kinase 3 alpha (GSK3A) is a multifunctional protein serine/threonine kinase implicated in the control of several regulatory proteins and transcription factors. (GSK3A Alias: DKFZp686D0638). Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequence identity and both of them were claimed to have an important role in human carcinogenesis. The GSTT1 gene is located approximately 50kb away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686D0638; Glycogen synthase kinase-3 alpha; GSK-3 alpha; gsk-3-alpha; OTTHUMP00000198368; Serine/threonine-protein kinase GSK3A
Gene Aliases: GSK3A
UniProt ID: (Human) P49840
Entrez Gene ID: (Human) 2931
regulation of systemic arterial blood pressure
cardiac left ventricle morphogenesis
glycogen metabolic process
protein phosphorylation
dopamine receptor signaling pathway
nervous system development
insulin receptor signaling pathway
negative regulation of UDP-glucose catabolic process
Wnt signaling pathway
positive regulation of cAMP biosynthetic process
negative regulation of TOR signaling
cellular response to insulin stimulus
cellular response to interleukin-3
IRE1-mediated unfolded protein response
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of glycogen biosynthetic process
positive regulation of protein catabolic process
positive regulation of heart contraction
negative regulation of glucose import
negative regulation of insulin receptor signaling pathway
negative regulation of cell growth involved in cardiac muscle cell development
positive regulation of adrenergic receptor signaling pathway
negative regulation of canonical Wnt signaling pathway
extrinsic apoptotic signaling pathway in absence of ligand
chemical synaptic transmission, postsynaptic
positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway
regulation of mitophagy
positive regulation of protein targeting to mitochondrion
negative regulation of glycogen synthase activity, transferring glucose-1-phosphate
negative regulation of type B pancreatic cell development
negative regulation of glycogen (starch) synthase activity
positive regulation of glycogen (starch) synthase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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