Invitrogen

Phospho-GSK3B (Ser9) Recombinant Rabbit Monoclonal Antibody (SY02-71)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

View all (87) GSK3B antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-32098

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SY02-71

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser9 of Human GSK3 beta aa 1-50
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2809391

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

GSK3B is a proline-directed serine/threonine protein kinase involved in energy metabolism, neuronal cell development and body pattern formation. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. GSK3B is a critical component of the Wnt/beta-catenin signaling pathway, and its activity results in the inhibition of Wnt signaling by mediating the degradation of beta-catenin. GSK3B is also involved in the Hedgehog, Notch, and insulin signaling pathways.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: EC 2.7.11.26; Glycogen synthase kinase-3 beta; GSK 3 beta; GSK 3B; GSK-3 beta; GSK-3 beta GSK3B; GSK-3b; GSK3 beta; GSK3beta isoform; Serine/threonine-protein kinase GSK3B

View more View less

Gene Aliases: GSK3B

View more View less

UniProt ID: (Human) P49841

View more View less

Entrez Gene ID: (Human) 2932

View more View less

Function(s)
RNA polymerase II transcription factor binding p53 binding protein kinase activity protein serine/threonine kinase activity integrin binding protein binding ATP binding beta-catenin binding kinase activity protein kinase binding ubiquitin protein ligase binding protein kinase A catalytic subunit binding ionotropic glutamate receptor binding tau protein binding tau-protein kinase activity NF-kappaB binding non-receptor serine/threonine protein kinase
Process(es)
re-entry into mitotic cell cycle epithelial to mesenchymal transition positive regulation of cell-matrix adhesion glycogen metabolic process regulation of gene expression by genetic imprinting protein phosphorylation protein export from nucleus ER overload response dopamine receptor signaling pathway axonogenesis myoblast fusion aging circadian rhythm organ morphogenesis negative regulation of cardiac muscle hypertrophy positive regulation of peptidyl-threonine phosphorylation positive regulation of mitochondrial membrane potential negative regulation of neuron maturation response to activity Wnt signaling pathway cell migration peptidyl-serine phosphorylation peptidyl-threonine phosphorylation hippocampus development establishment of cell polarity negative regulation of protein complex assembly positive regulation of protein complex assembly negative regulation of protein binding positive regulation of protein binding response to estradiol positive regulation of proteasomal ubiquitin-dependent protein catabolic process response to insulin regulation of microtubule-based process positive regulation of peptidyl-serine phosphorylation negative regulation of smooth muscle cell apoptotic process protein localization to microtubule intracellular signal transduction cellular response to hepatocyte growth factor stimulus cellular response to interleukin-3 cellular response to erythropoietin response to drug negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of MAP kinase activity positive regulation of neuron apoptotic process positive regulation of GTPase activity hypermethylation of CpG island canonical Wnt signaling pathway involved in positive regulation of apoptotic process fat cell differentiation negative regulation of glycogen biosynthetic process positive regulation of protein catabolic process positive regulation of axon extension positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation positive regulation of protein export from nucleus regulation of neuronal synaptic plasticity positive regulation of smooth muscle cell proliferation negative regulation of dendrite morphogenesis negative regulation of nitric-oxide synthase activity negative regulation of NFAT protein import into nucleus canonical Wnt signaling pathway intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to hydrogen peroxide superior temporal gyrus development cellular response to mechanical stimulus cellular response to iron(II) ion cellular response to lithium ion response to epinephrine negative regulation of canonical Wnt signaling pathway extrinsic apoptotic signaling pathway in absence of ligand chemical synaptic transmission, postsynaptic regulation of cellular response to heat negative regulation of protein localization to nucleus positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway positive regulation of neuron death response to L-glutamate cellular response to dopamine negative regulation of dopaminergic neuron differentiation beta-catenin destruction complex assembly beta-catenin destruction complex disassembly response to insulin-like growth factor stimulus response to ultrasound response to angiotensin negative regulation of type B pancreatic cell development positive regulation of excitatory postsynaptic potential negative regulation of glycogen (starch) synthase activity positive regulation of DNA biosynthetic process positive regulation of stem cell differentiation negative regulation of neuron migration
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support