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Phospho-HTRA2 (Ser212) Antibody (PA5-118695) in IHC (P)
Immunohistochemical analysis of Phospho-HTRA2 (Ser212) in Mouse kidney tissue using a Phospho-HTRA2 (Ser212) polyclonal antibody (Product # PA5-118695). The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the second... View More
Product Details
PA5-118695
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human HTRA2(Accession O43464), corresponding to amino acid residues around phosphorylated Ser212.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2903195
Product Specific Information
Antibody detects endogenous levels of HtrA2 only when phosphorylated at Ser212.
Target Information
The inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif that is essential for their anti-apoptotic activity (1-3). Recently, a serine protease has been isolated, which is released from the mitochondria upon induction of apoptosis by apoptotic stimuli. This protein called Omi/HtrA2 is a mitochondrial protein that binds to IAP (4). Like Smac/DIABLO, the mature Omi protein contains a conserved IAP-binding motif (AVPS) at its N terminus. The deregulated expression of Omi in the cytoplasm of mammalian cells induces apoptosis indicating that Omi could participate in the mitochondrial apoptotic pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: High temperature requirement protein A2; HtrA-like serine protease; HtrA2; motor neuron degeneration 2; Omi stress-regulated endoprotease; protease, serine, 25; Serine protease 25; Serine protease HTRA2, mitochondrial; serine protease OMI; Serine proteinase OMI
Gene Aliases: AI481710; HTRA2; mnd2; OMI; PARK13; PRSS25
UniProt ID: (Human) O43464, (Mouse) Q9JIY5
Entrez Gene ID: (Human) 27429, (Rat) 297376, (Mouse) 64704
proteolysis
ceramide metabolic process
mitochondrion organization
adult walking behavior
intrinsic apoptotic signaling pathway in response to DNA damage
response to herbicide
positive regulation of cell death
protein autoprocessing
pentacyclic triterpenoid metabolic process
forebrain development
cellular response to oxidative stress
cellular response to heat
cellular response to interferon-beta
regulation of multicellular organism growth
positive regulation of apoptotic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
cellular protein catabolic process
negative regulation of cell cycle
neuron development
cellular response to retinoic acid
cellular response to growth factor stimulus
execution phase of apoptosis
negative regulation of neuron death
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
regulation of mitophagy
positive regulation of protein targeting to mitochondrion
positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway
adult locomotory behavior
negative regulation of cell death
intrinsic apoptotic signaling pathway
apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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