Invitrogen

Phospho-Histone H2A (Ser139) Monoclonal Antibody (GT1021)

Phospho-Histone H2A (Ser139) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-Histone H2A (Ser139) Antibody (MA5-31471) in ICC/IF

Phospho-Histone H2A (Ser139) Monoclonal Antibody (GT1021) detects Histone H2A. XS139ph (phospho Ser139) protein at Nucleus by immunofluorescent analysis. Sample: 10 µM Cisplatin for 24 hrs HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: Histone H2A. XS139ph (phospho Ser139) protein stained by Phospho-Histone H2A (Ser139) Monoclonal Antibody (GT1021) (Product # MA5-31471) diluted at 1:500... View More

Phospho-Histone H2A (Ser139) Antibody in Western Blot (WB)

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Product Details

MA5-31471

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

GT1021

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human Histone H2A.XS139ph (phospho Ser139).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.05 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2787103

Product Specific Information

Keep as concentrated solution.

Positive Control: HCT116 (30 µM Cisplatin treatment for 24hr).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A noncanonical response to replication stress protects genome stability through ROS production, in an adaptive manner.

Cell death and differentiation

Ragu S,Droin N,Matos-Rodrigues G,Barascu A,Caillat S,Zarkovic G,Siberchicot C,Dardillac E,Gelot C,Guirouilh-Barbat J,Radicella JP,Ishchenko AA,Ravanat JL,Solary E,Lopez BS

MA5-31471 was used in Western Blotting to characterize a noncanonical cellular response that is specific to nonblocking replication stress.

Mon May 01 00:00:00 EDT 2023

Bioinformatics

Protein Aliases: Acetyl-Histone H2; H2A 615 antibody; H2A GL101 antibody; H2A histone family member; H2A histone family, member A; H2A histone family, member C; H2A histone family, member E; H2A histone family, member G; H2A histone family, member M; H2A histone family, member P; H2A histone family, member R; H2A histone family, member X; H2A histone family, member Z; H2A-clustered histone 1; H2A-clustered histone 12; H2A-clustered histone 6; H2A.1; H2A.1 antibody; H2A/d; H2a/x; H2A/z; h2ad; H2ae; h2ai; H2ak; H2AK5ac; H2AX histone; H2AZ histone; H2B histone family, member U, (testis-specific); HIS; HIST1H2AE antibody; HIST3H2A; histone 1, H2aa; histone 1, H2ab; histone 1, H2ad; histone 1, H2ae; histone 1, H2ag; histone 1, H2ah; histone 1, H2ai; histone 1, H2aj; histone 1, H2ba; histone 2, H2ai; histone 5 protein 2ax; Histone H2A type 1; Histone H2A type 1-A; Histone H2A type 1-B; Histone H2A type 1-B/E; Histone H2A type 1-C; Histone H2A type 1-D; Histone H2A type 1-E; Histone H2A type 1-G; Histone H2A type 1-H; Histone H2A type 1-I; Histone H2A type 1-J; Histone H2A type 1-N; Histone H2A type 1-O; Histone H2A type 1-P; Histone H2A.2; Histone H2A.3; Histone H2A.X; Histone H2A.Z; Histone H2A/a; Histone H2A/e; Histone H2A/g; Histone H2A/m; histone H2A/p; Histone H2A/ptl; Histone H2A/r; Histone H2A/s; histone H2AD; histone H2AE; Histone H2AX; Histone H2B type 1-A; Histone H2B, testis; MGC74460 antibody; Testis-specific histone H2B; TSH2B.1

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Gene Aliases: 1190022L06; AW228881; bA317E16.2; bA317E16.3; dJ160A22.4; dJ86C11.1; gammaH2ax; H2a-291A; H2a-53; H2A.1; H2A.1b; H2A.2; H2A.3; H2A.X; H2A.Z; H2A.Z-1; H2A/a; H2A/c; H2A/E; H2A/g; H2A/m; H2A/p; H2A/S; H2A/X; H2A/z; H2AA; H2AC1; H2AC11; H2AC12; H2AC13; H2AC14; H2AC15; H2AC16; H2AC17; H2AC4; H2ac6; H2AC7; H2AC8; H2AFA; H2AFALii; H2AFC; H2AFD; H2AFE; H2AFG; H2AFI; H2AFM; H2AFN; H2AFP; H2AFR; H2AFX; H2AFZ; H2AG; H2AH; H2AX; H2AZ; H2AZ1; H2BC1; H2BFU; HIST1H2AA; HIST1H2AB; Hist1h2ac; HIST1H2AD; HIST1H2AE; HIST1H2AG; HIST1H2AH; HIST1H2AI; HIST1H2AJ; HIST1H2AK; HIST1H2AL; HIST1H2AM; Hist1h2an; Hist1h2ao; Hist1h2ap; HIST1H2BA; Hist5-2ax; pH2A/f; STBP; TH2A; TH2B; TSH2B; TSH2B.1

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UniProt ID: (Human) P16104, (Human) P0C0S5, (Mouse) P27661, (Mouse) P0C0S6, (Human) Q96QV6, (Human) P04908, (Human) P20671, (Human) P0C0S8, (Human) Q96KK5, (Human) Q99878, (Human) Q96A08, (Mouse) C0HKE6, (Mouse) Q8CGP6

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Function(s)

DNA binding damaged DNA binding protein binding enzyme binding histone binding protein heterodimerization activity RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding chromatin DNA binding nucleosomal DNA binding molecular_function histone

Process(es)

DNA damage checkpoint double-strand break repair via homologous recombination double-strand break repair double-strand break repair via nonhomologous end joining nucleosome assembly chromatin silencing cellular response to DNA damage stimulus spermatogenesis response to ionizing radiation viral process cerebral cortex development positive regulation of DNA repair meiotic cell cycle cellular response to gamma radiation cellular senescence positive regulation of transcription from RNA polymerase II promoter cellular response to estradiol stimulus DNA repair DNA recombination cell cycle multicellular organism development cellular response to insulin stimulus negative regulation of cell proliferation biological_process nucleosome disassembly inflammatory response plasminogen activation spermatogenesis, exchange of chromosomal proteins positive regulation of binding mononuclear cell migration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-Histone H2A (Ser139) Monoclonal Antibody (GT1021)

Phospho-Histone H2A (Ser139) Antibody (MA5-31471) in ICC/IF

Product Details

MA5-31471

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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