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7TM Antibodies
Phospho-Hydroxycarboxylic Acid Receptor 2 (Thr338) Polyclonal Antibody
FIGURE: 1 / 1
Phospho-Hydroxycarboxylic Acid Receptor 2 (Thr338) Antibody (7TM0312A) in WB
Agonist-induced Threonine338 phosphorylation of the Hydroxycarboxylic Acid Receptor 2. Upper panel, HEK293 cells stably expressing the Hydroxycarboxylic acid Receptor 2 (HCA2) were either not exposed or exposed to 100 nM Nicotinic Acid for 30 minutes. Cells were lysed and immunoblotted with the anti-pT338-HCA2 antibody (7TM0312A) at a dilution of 1:1,000. Lower panel, blot was stripped and reprobed with an ... View More
Product Details
7TM0312A
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic phosphopeptide derived from human HCA2 around the phosphorylation site of Thr338.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.4
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice (domestic); Dry ice (international)
Target Information
GPR109A (also known as HCA2, HM74a, and NIACR1) is a 42kDa G-protein coupled receptor that belongs to a family of three hydroxyl-carboxylic acid receptors, which also include GPR109B and GPR81. GPR109A has two known ligands: niacin and butyrate (or its derivative 3-hydroxybutyrate). The endogenous ligands for GPR109B and GPR81 are 3-hydroxyl-octanoic acid and 2-hydroxy-propanoate (lactate), respectively. GPR109A is expressed on adipocytes, neutrophils, Langerhans cells, keratinocytes, intestinal epithelial cells, and intestinal colonic macrophages. Niacin, also known as nicotinic acid or vitamin B3, is known to improve cholesterol metabolism (LDL/HDL ratio) and reduce inflammation. It has been demonstrated that this effect is mediated through GPR109A. Notably, GPR109A also has an essential role in suppressing inflammation and cancer in the colon. Both GPR109A ligands are produced by colonic commensal bacteria. In mice, GPR109A expression on intestinal epithelial cells and DCs/macrophages contributes to the suppression of colitis and colon cancer. In humans, GPR109A is silenced in colon carcinoma cells by DNA methylation, which increases inflammation in the intestinal tract.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G protein-coupled receptor 109A; G protein-coupled receptor HM74a; G-protein coupled receptor 109A; G-protein coupled receptor HM74A; hydroxy-carboxylic acid receptor 2; Hydroxycarboxylic acid receptor 2; Niacin receptor 1; Nicotinic acid receptor; PUMA-G. GPR109
Gene Aliases: GPR109A; HCA2; HCAR2; HM74A; HM74b; NIACR1; Puma-g; PUMAG
UniProt ID: (Human) Q8TDS4
Entrez Gene ID: (Human) 338442
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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