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Phospho-IL-1R1 (Tyr496) Antibody (BS-5394R) in Flow
MCF-7 cells were fixed with 70% methanol (overnight at 4°C). The cells were then incubated in 1X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Primary incubation with IL-1R1 (Tyr496) Polyclonal Antibody, Unconjugated (bs-5349R) at 1:100 (1 µg /10^6 cells) for 30 minutes at room temp. A PE-conjugated secondary antibody (bs-0295G-PE) was used for ... View More
Product Details
BS-5394R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic phosphopeptide derived from human IL-1R1 around the phosphorylation site of Tyr496.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
The IL-1 system includes two agonists (IL-1 and IL-1), converting enzymes, antagonists, two receptors (IL-1 RI and IL-1 RII) and the IL-1 receptor accessory protein. Interleukin-1 signal is transduced through the type I receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: antigen CD121a; CD121 alpha; CD121 antigen-like family member A; CD121a; il-1 receptor; IL-1 receptor alpha chain; il-1r; IL-1R-1; IL-1R-alpha; IL-1RT-1; IL-1RT1; il1 receptor; interleukin 1 beta receptor type 1 short transmembrane form; interleukin 1 beta receptor type 1 soluble form; interleukin 1 bete receptor type 1 long transmembrane form; interleukin 1 receptor alpha, type I; interleukin 1 receptor, type I; interleukin-1 receptor; Interleukin-1 receptor alpha; Interleukin-1 receptor type 1; Interleukin-1 receptor type I; p80
Gene Aliases: CD121A; CD121b; D2S1473; IL-1R-alpha; IL-1R1; IL-iR; IL1R; Il1r-1; IL1R1; IL1RA; IL1RT1; P80
UniProt ID: (Human) P14778, (Mouse) P13504
Entrez Gene ID: (Human) 3554, (Mouse) 16177, (Rat) 25663
protease binding
signal transducer activity
transmembrane signaling receptor activity
interleukin-1 receptor activity
interleukin-1, Type I, activating receptor activity
platelet-derived growth factor receptor binding
protein binding
ionotropic glutamate receptor binding
transmembrane signal receptor
immune response
cell surface receptor signaling pathway
response to radiation
heat acclimation
ovulation
regulation of inflammatory response
interleukin-1-mediated signaling pathway
response to interleukin-1
response to epidermal growth factor
cellular response to glucose stimulus
response to transforming growth factor beta
response to nitric oxide
signal transduction
cytokine-mediated signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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