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Invitrogen
Phospho-IR/IGF1R (Tyr1158, Tyr1162, Tyr1163) Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
Product Details
44-806G
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Chicken,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phosphopeptide derived from the region of IR/IGF1R that contains tyrosines 1158, 1162 and 1163 of the human insulin receptor (IR)
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533763
Target Information
Insulin and Insulin-like Growth Factor receptors are transmembrane tyrosine kinase receptors that play critical roles in development, cell growth and metabolism. Insulin/Insulin-like growth factor-1 binding to the extracellular domain of IR and IGFR leads to autophosphorylation of the receptor and activation of the intrinsic tyrosine kinase activity, which allows appropriate substrates to be phosphorylated. Phosphorylation sites that are unique to each receptor presumably play a key role in these signaling differences. The catalytic loops within the tyrosine kinase domains contain a three tyrosine motif corresponding to Tyr1158, 1162 and 1163 (for the IR) and Tyr1131, 1135 and 1136 (for the IGF1R). Autophosphorylation of the tyrosine sites within the activation loop proceeds in a processive manner initiating at the second tyrosine (1162 or 1135), followed by phosphorylation at tyrosines 1158 (IGF-1R tyrosine 1131), 1163 (IGF-1R tyrosine 1136), resulting in the full activation of IR and IGF1R.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD220; CD221; IGF-I receptor; IGF-I Receptor beta; IGF1 Receptor; IGF1 Receptor beta; IGF1R beta; Insulin receptor; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; IR; line 186; soluble IGF1R variant 1; soluble IGF1R variant 2
Gene Aliases: 4932439J01Rik; A330103N21Rik; CD220; CD221; D630014A15Rik; D930020L01; HHF5; hyft; IGF-1R; IGF1R; IGFIR; IGFR; INSR; IR; IR-A; IR-B; JTK13
UniProt ID: (Human) P08069, (Human) P06213, (Mouse) Q62123, (Mouse) P15208
Entrez Gene ID: (Human) 3480, (Human) 3643, (Mouse) 16001, (Mouse) 16337
protein tyrosine kinase activity
insulin-like growth factor-activated receptor activity
insulin receptor binding
protein binding
insulin-like growth factor binding
ATP binding
insulin-like growth factor I binding
identical protein binding
phosphatidylinositol 3-kinase binding
insulin binding
insulin receptor substrate binding
receptor signaling protein tyrosine kinase activity
insulin-activated receptor activity
insulin-like growth factor receptor binding
GTP binding
protein phosphatase binding
lipoic acid binding
insulin-like growth factor II binding
protein complex binding
3-phosphoinositide-dependent protein kinase binding
PTB domain binding
nucleotide binding
G-protein alpha-subunit binding
protein kinase activity
transmembrane receptor protein tyrosine kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein kinase binding
protein domain specific binding
transmembrane signal receptor
immune response
signal transduction
positive regulation of cell proliferation
insulin receptor signaling pathway
phosphatidylinositol 3-kinase signaling
positive regulation of cell migration
peptidyl-tyrosine autophosphorylation
negative regulation of apoptotic process
positive regulation of DNA replication
regulation of JNK cascade
protein autophosphorylation
insulin-like growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
protein tetramerization
inactivation of MAPKK activity
activation of MAPK activity
negative regulation of protein phosphorylation
positive regulation of protein phosphorylation
heart morphogenesis
carbohydrate metabolic process
regulation of transcription, DNA-templated
G-protein coupled receptor signaling pathway
epidermis development
male gonad development
response to manganese ion
regulation of hydrogen peroxide metabolic process
positive regulation of glycoprotein biosynthetic process
negative regulation of gene expression
response to activity
peptidyl-tyrosine phosphorylation
transformation of host cell by virus
signal transduction by protein phosphorylation
male sex determination
adrenal gland development
exocrine pancreas development
activation of protein kinase activity
activation of protein kinase B activity
response to estradiol
negative regulation of transporter activity
cellular response to insulin stimulus
response to vitamin D
response to testosterone
response to tumor necrosis factor
glucose homeostasis
positive regulation of MAPK cascade
positive regulation of nitric oxide biosynthetic process
fat cell differentiation
response to ethanol
positive regulation of glycogen biosynthetic process
positive regulation of glycolytic process
positive regulation of mitotic nuclear division
positive regulation of transcription, DNA-templated
regulation of embryonic development
positive regulation of glucose import
positive regulation of developmental growth
protein heterotetramerization
response to glucocorticoid
positive regulation of meiotic cell cycle
positive regulation of protein kinase B signaling
positive regulation of respiratory burst
cellular response to growth factor stimulus
regulation of female gonad development
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
axonogenesis
brain development
organ morphogenesis
negative regulation of muscle cell apoptotic process
phosphorylation
establishment of cell polarity
mammary gland development
neuron projection development
positive regulation of cytokinesis
negative regulation of MAPK cascade
negative regulation of sequence-specific DNA binding transcription factor activity
positive regulation of DNA metabolic process
protein heterooligomerization
negative regulation of protein kinase B signaling
prostate gland epithelium morphogenesis
positive regulation of steroid hormone biosynthetic process
intracellular signal transduction
positive regulation of phosphorylation
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