Invitrogen

Phospho-IR/IGF1R (Tyr1162, Tyr1163) Recombinant Rabbit Monoclonal Antibody (97H9L7)

1 Published Figure
4 References
View all (9) IR/IGF1R antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

700393

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

-
View 1 publication 1 publication

ELISA (ELISA)

1-5 µg/mL
-
Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

97H9L7

Immunogen

A peptide corresponding to amino acids 1186-1197 and 1162-1173 of P06213 and P08069, respectively
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532321

Product Specific Information

This antibody is predicted to react with bovine, canine, chicken, chimpanzee, equine, mouse, rat, Rhesus monkey , porcine and Xenopus based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Insulin and Insulin-like Growth Factor receptors are transmembrane tyrosine kinase receptors that play critical roles in development, cell growth and metabolism. Insulin/Insulin-like growth factor-1 binding to the extracellular domain of IR and IGFR leads to autophosphorylation of the receptor and activation of the intrinsic tyrosine kinase activity, which allows appropriate substrates to be phosphorylated. Phosphorylation sites that are unique to each receptor presumably play a key role in these signaling differences. The catalytic loops within the tyrosine kinase domains contain a three tyrosine motif corresponding to Tyr1158, 1162 and 1163 (for the IR) and Tyr1131, 1135 and 1136 (for the IGF1R). Autophosphorylation of the tyrosine sites within the activation loop proceeds in a processive manner initiating at the second tyrosine (1162 or 1135), followed by phosphorylation at tyrosines 1158 (IGF-1R tyrosine 1131), 1163 (IGF-1R tyrosine 1136), resulting in the full activation of IR and IGF1R.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD220; CD221; IGF-I receptor; IGF-I Receptor beta; IGF1 Receptor; IGF1 Receptor beta; IGF1R beta; Insulin receptor; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; IR; soluble IGF1R variant 1; soluble IGF1R variant 2

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Gene Aliases: CD220; CD221; HHF5; IGF1R; IGFIR; IGFR; INSR; JTK13

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UniProt ID: (Human) P08069, (Human) P06213

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Entrez Gene ID: (Human) 3480, (Human) 3643

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Function(s)
protein tyrosine kinase activity insulin-like growth factor-activated receptor activity insulin receptor binding protein binding insulin-like growth factor binding ATP binding insulin-like growth factor I binding identical protein binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding receptor signaling protein tyrosine kinase activity insulin-activated receptor activity insulin-like growth factor receptor binding GTP binding protein phosphatase binding lipoic acid binding insulin-like growth factor II binding protein complex binding 3-phosphoinositide-dependent protein kinase binding PTB domain binding transmembrane signal receptor
Process(es)
immune response signal transduction positive regulation of cell proliferation insulin receptor signaling pathway phosphatidylinositol 3-kinase signaling positive regulation of cell migration peptidyl-tyrosine autophosphorylation negative regulation of apoptotic process positive regulation of DNA replication regulation of JNK cascade protein autophosphorylation insulin-like growth factor receptor signaling pathway phosphatidylinositol-mediated signaling protein tetramerization inactivation of MAPKK activity activation of MAPK activity negative regulation of protein phosphorylation positive regulation of protein phosphorylation heart morphogenesis carbohydrate metabolic process regulation of transcription, DNA-templated G-protein coupled receptor signaling pathway epidermis development male gonad development response to manganese ion regulation of hydrogen peroxide metabolic process positive regulation of glycoprotein biosynthetic process negative regulation of gene expression response to activity peptidyl-tyrosine phosphorylation transformation of host cell by virus signal transduction by protein phosphorylation male sex determination adrenal gland development exocrine pancreas development activation of protein kinase activity activation of protein kinase B activity response to estradiol negative regulation of transporter activity cellular response to insulin stimulus response to vitamin D response to testosterone response to tumor necrosis factor glucose homeostasis positive regulation of MAPK cascade positive regulation of nitric oxide biosynthetic process fat cell differentiation response to ethanol positive regulation of glycogen biosynthetic process positive regulation of glycolytic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated regulation of embryonic development positive regulation of glucose import positive regulation of developmental growth protein heterotetramerization response to glucocorticoid positive regulation of meiotic cell cycle positive regulation of protein kinase B signaling positive regulation of respiratory burst cellular response to growth factor stimulus regulation of female gonad development
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