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Invitrogen
Phospho-IRE1 alpha (Ser724) Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
Phospho-IRE1 alpha (Ser724) Antibody (PA5-85738) in WB
Western Blot was performed using Anti-Phospho-IRE1 alpha (Ser724) Polyclonal Antibody (Product # PA5-85738) and a 110 kDa band corresponding to Serine/threonine-protein kinase/endoribonuclease IRE1 was observed in HeLa cells treated with EGF. Membrane enriched extracts (30 µg lysate) of HeLa (Lane 1) and HeLa treated with 100 ng/mL of EGF for 20 mins (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tri... View More
Product Details
PA5-85738
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide corresponding to residues around human IRE1 (phospho Ser724).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2792877
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (100%), Rat (100%), Japanese Medaka (100%).
Positive Control: Neuro2A, C8D30, NIH-3T3, C2C12, Rat2, HepG2, HepG2 (3uM Thapsigargin treatment for 12 hr).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ERN1 serine/threonine kinase or endoplasmic reticulum to nucleus signaling 1 is the ER to nucleus signaling 1 protein, a human homologue of the yeast Ire1 gene product which possesses intrinsic kinase activity and an endoribonuclease activity and it is important in endoplasmic reticulum-based stress signals. ERN1 controls IRE1 proteolysis in mammalian cells. ERN1 has a critical function in extraembryonic cells that is essential for fetal viability. Specifically, IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator. Null mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: endoplasmic reticulum (ER) to nucleus signalling 1; Endoplasmic reticulum-to-nucleus signaling 1; ER to nucleus signalling 1; ERN1; FLJ30999; hIRE1p; inositol-requiring 1; inositol-requiring 1 alpha; inositol-requiring enzyme 1; Inositol-requiring protein 1; Ire1-alpha; IRE1a; IRE1a antibody; IRE1p antibody; MGC163277; MGC163279; protein kinase/endoribonuclease; Serine/threonine-protein kinase/endoribonuclease IRE1
Gene Aliases: 9030414B18Rik; AI225830; C85377; ERN1; hIRE1p; IRE1; IRE1a; Ire1alpha; IRE1P; RGD1559716
UniProt ID: (Human) Q59EE2, (Mouse) Q9EQY0
Entrez Gene ID: (Human) 2081, (Mouse) 78943, (Rat) 498013
magnesium ion binding
endoribonuclease activity
protein serine/threonine kinase activity
protein binding
ATP binding
enzyme binding
Hsp70 protein binding
identical protein binding
protein homodimerization activity
ADP binding
Hsp90 protein binding
nucleotide binding
catalytic activity
endonuclease activity
ribonuclease activity
protein kinase activity
kinase activity
bidentate ribonuclease III activity
transferase activity
transferase activity, transferring phosphorus-containing groups
hydrolase activity
metal ion binding
endoribonuclease
endothelial cell proliferation
transcription, DNA-templated
regulation of transcription, DNA-templated
mRNA cleavage
mRNA catabolic process
protein phosphorylation
activation of signaling protein activity involved in unfolded protein response
cell cycle arrest
activation of JUN kinase activity
regulation of macroautophagy
positive regulation of RNA splicing
response to endoplasmic reticulum stress
cellular response to vascular endothelial growth factor stimulus
peptidyl-serine autophosphorylation
IRE1-mediated unfolded protein response
protein autophosphorylation
mRNA splicing, via endonucleolytic cleavage and ligation
mRNA endonucleolytic cleavage involved in unfolded protein response
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to glucose stimulus
RNA phosphodiester bond hydrolysis, endonucleolytic
positive regulation of endoplasmic reticulum unfolded protein response
insulin metabolic process
peptidyl-serine trans-autophosphorylation
mRNA processing
apoptotic process
response to unfolded protein
metabolic process
phosphorylation
endoplasmic reticulum unfolded protein response
mRNA splicing via endonucleolytic cleavage and ligation involved in unfolded protein response
RNA splicing
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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