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Phospho-IRS1 (Ser307) Antibody (600-401-445) in WB
Western blot using Rocklands Affinity Purified anti-IRS1 pS307 antibody shows detection of a band at ~180 kDa believed to represent phosphorylated IRS1 (arrowhead). Lane 1 shows staining of human 293 cell lysate. Lane 2 shows staining of 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 µg/ml of anisomysin for 30 min. The pronounced staining of the band at 180 kDa is no... View More
Product Details
600-401-445
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 290-320 of human IRS1 protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against the phosphorylated form of human IRS1 protein at the pS307 residue. Reactivity occurs against human IRS1 pS307 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human IRS1 is minimal by ELISA. A BLAST analysis was used to suggest cross reactivity with IRS1 from human, mouse, rat, dog and vervet monkey based on 100% homology with the immunizing sequence. Partial reactivity is also expected against IRS1 from pig (94%), bovine (94%) and chicken (88%) sources. Reactivity of this antibody with IRS1 from other species is unknown.
Target Information
IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Insulin receptor substrate 1; IRS-1
Gene Aliases: HIRS-1; IRS1
UniProt ID: (Human) P35568
Entrez Gene ID: (Human) 3667
signal transducer activity
transmembrane receptor protein tyrosine kinase adaptor activity
protein kinase C binding
Ras guanyl-nucleotide exchange factor activity
insulin receptor binding
insulin-like growth factor receptor binding
protein binding
1-phosphatidylinositol-3-kinase activity
SH2 domain binding
phosphatidylinositol 3-kinase binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
MAPK cascade
signal transduction
positive regulation of cell proliferation
insulin receptor signaling pathway
positive regulation of glucose metabolic process
phosphatidylinositol 3-kinase signaling
regulation of phosphatidylinositol 3-kinase signaling
positive regulation of fatty acid beta-oxidation
response to insulin
cellular response to insulin stimulus
phosphatidylinositol-3-phosphate biosynthetic process
glucose homeostasis
response to peptide hormone
positive regulation of GTPase activity
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of glycogen biosynthetic process
positive regulation of glucose import
negative regulation of insulin receptor signaling pathway
positive regulation of insulin receptor signaling pathway
negative regulation of insulin secretion
phosphatidylinositol phosphorylation
insulin-like growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
positive regulation of glucose import in response to insulin stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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