Rockland

Phospho-Insulin Receptor (Tyr1361) Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

600-401-MR0

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (IHC)

Assay-dependent
-

ELISA (ELISA)

1:10,000-1:50,000
-

Dot blot (DB)

1:70,000.
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-Insulin Receptor pY1361 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal portion near the phosphorylation Y1361 of the human Insulin Receptor conjugated to Keyhole Limpet Hemocyanin (KLH).
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against human Insulin Receptor. This phospho specific polyclonal antibody is specific for pY1361 human Insulin Receptor. Reactivity with non-phosphorylated Y1361 is minimal. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human, rat, mouse, and xenopus laevis.

Target Information

Biological actions of insulin and IGF-1 are mediated by their respective cell surface receptor tyrosine kinases that regulate multiple signaling pathways through activation of a series of phosphorylation cascades. The insulin receptor. Insulin/IGF-1 binding to the extracellular domain leads to autophosphorylation of downstream target proteins. These two receptors differ in sequence in regions that confer specificity for the designated ligand as well as in certain intracellular signaling domains, resulting in significant differences in the functional consequences of activation of each receptor. Defects in IR are the cause of various insulin resistance syndromes and IGF-1R defects may cause some forms of growth retardation. Both these signaling cascades are also important for the development of cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD220; CD220 beta; Insulin receptor; IR; IR beta

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Gene Aliases: CD220; HHF5; INSR

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UniProt ID: (Human) P06213

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Entrez Gene ID: (Human) 3643

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Function(s)
protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity insulin-activated receptor activity insulin-like growth factor receptor binding protein binding ATP binding GTP binding protein phosphatase binding lipoic acid binding insulin-like growth factor I binding insulin-like growth factor II binding protein complex binding 3-phosphoinositide-dependent protein kinase binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding PTB domain binding transmembrane signal receptor
Process(es)
activation of MAPK activity negative regulation of protein phosphorylation positive regulation of protein phosphorylation heart morphogenesis carbohydrate metabolic process regulation of transcription, DNA-templated G-protein coupled receptor signaling pathway positive regulation of cell proliferation insulin receptor signaling pathway epidermis development male gonad development response to manganese ion regulation of hydrogen peroxide metabolic process positive regulation of glycoprotein biosynthetic process negative regulation of gene expression response to activity peptidyl-tyrosine phosphorylation transformation of host cell by virus signal transduction by protein phosphorylation male sex determination adrenal gland development positive regulation of cell migration exocrine pancreas development activation of protein kinase activity activation of protein kinase B activity response to estradiol negative regulation of transporter activity cellular response to insulin stimulus response to vitamin D response to testosterone response to tumor necrosis factor peptidyl-tyrosine autophosphorylation glucose homeostasis positive regulation of MAPK cascade positive regulation of nitric oxide biosynthetic process fat cell differentiation response to ethanol positive regulation of glycogen biosynthetic process positive regulation of DNA replication positive regulation of glycolytic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated regulation of embryonic development positive regulation of glucose import protein autophosphorylation positive regulation of developmental growth protein heterotetramerization response to glucocorticoid positive regulation of meiotic cell cycle positive regulation of protein kinase B signaling positive regulation of respiratory burst cellular response to growth factor stimulus regulation of female gonad development
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