Invitrogen

Phospho-LAT (Tyr132) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

8 Published Figures

10 References

View all (31) LAT antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Phospho-LAT (Tyr132) Antibody in Western Blot (WB)

FIGURE: 1 / 10

Phospho-LAT (Tyr132) Antibody (44-224) in WB

Peptide Competition: Extracts prepared from Jurkat E6.1 cells were left unstimulated (1) or stimulated (2-5), resolved by SDS-PAGE on a 10% polyacrylamide gel, and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C.

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Product Details

44-224

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 9 publications 9 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human LAT that contains tyrosine 132.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533608

Target Information

LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Aging-associated HELIOS deficiency in naive CD4⁺ T cells alters chromatin remodeling and promotes effector cell responses.

Nature immunology

Zhang H,Jadhav RR,Cao W,Goronzy IN,Zhao TV,Jin J,Ohtsuki S,Hu Z,Morales J,Greenleaf WJ,Weyand CM,Goronzy JJ

44-224 was used in Western Blotting to identify increased phosphorylation of STAT5, at least in part due to aberrant IL-2 receptor and lower HELIOS expression, as upstream regulators.

Sun Jan 01 00:00:00 EST 2023

Kinetic proofreading through the multi-step activation of the ZAP70 kinase underlies early T cell ligand discrimination.

Nature immunology

Voisinne G,Locard-Paulet M,Froment C,Maturin E,Menoita MG,Girard L,Mellado V,Burlet-Schiltz O,Malissen B,Gonzalez de Peredo A,Roncagalli R

44-224 was used in Western Blotting to provide a comprehensive map of molecular events associated with T cell ligand discrimination.

Thu Sep 01 00:00:00 EDT 2022

PLCγ1 promotes phase separation of T cell signaling components.

The Journal of cell biology

Zeng L,Palaia I,Šarić A,Su X

44-224 was used in Western Blot to report that phospholipase Cγ1 (PLCγ1) promotes phase separation of LAT, a key adaptor protein in the T cell receptor pathway.

Mon Jun 07 00:00:00 EDT 2021

PLCγ1 promotes phase separation of T cell signaling components.

The Journal of cell biology

Zeng L,Palaia I,Šarić A,Su X

44-224 was used in Western Blot to report that phospholipase Cγ1 (PLCγ1) promotes phase separation of LAT, a key adaptor protein in the T cell receptor pathway.

Mon Jun 07 00:00:00 EDT 2021

The Cish SH2 domain is essential for PLC-γ1 regulation in TCR stimulated CD8⁺ T cells.

Scientific reports

Guittard G,Dios-Esponera A,Palmer DC,Akpan I,Barr VA,Manna A,Restifo NP,Samelson LE

44-224 was used in Immunocytochemistry to study the structural and functional relationships between Crish and PLC-γ1 during CD8+ T cell activation.

Wed Mar 28 00:00:00 EDT 2018

LFA-1 activates focal adhesion kinases FAK1/PYK2 to generate LAT-GRB2-SKAP1 complexes that terminate T-cell conjugate formation.

Nature communications

Raab M,Lu Y,Kohler K,Smith X,Strebhardt K,Rudd CE

Published figure using Phospho-LAT (Tyr132) polyclonal antibody (Product # 44-224) in Western Blot

Wed Jul 12 00:00:00 EDT 2017

Small molecule inhibition of Csk alters affinity recognition by T cells.

eLife

Manz BN,Tan YX,Courtney AH,Rutaganira F,Palmer E,Shokat KM,Weiss A

Published figure using Phospho-LAT (Tyr132) polyclonal antibody (Product # 44-224) in Western Blot

Mon Aug 24 00:00:00 EDT 2015

Inhibition of the kinase Csk in thymocytes reveals a requirement for actin remodeling in the initiation of full TCR signaling.

Nature immunology

Tan YX,Manz BN,Freedman TS,Zhang C,Shokat KM,Weiss A

Published figure using Phospho-LAT (Tyr132) polyclonal antibody (Product # 44-224) in Western Blot

Sat Feb 01 00:00:00 EST 2014

Quantitative and temporal requirements revealed for Zap70 catalytic activity during T cell development.

Nature immunology

Au-Yeung BB,Melichar HJ,Ross JO,Cheng DA,Zikherman J,Shokat KM,Robey EA,Weiss A

44-224 was used in western blot to examine the quantitative and temporal requirements of Zap70's function in thymocyte development

Tue Jul 01 00:00:00 EDT 2014

Stability of an autoinhibitory interface in the structure of the tyrosine kinase ZAP-70 impacts T cell receptor response.

Proceedings of the National Academy of Sciences of the United States of America

Deindl S,Kadlecek TA,Cao X,Kuriyan J,Weiss A

Tue Dec 08 00:00:00 EST 2009

Intramolecular regulatory switch in ZAP-70: analogy with receptor tyrosine kinases.

Molecular and cellular biology

Brdicka T,Kadlecek TA,Roose JP,Pastuszak AW,Weiss A

Wed Jun 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: 36 kDa phospho-tyrosine adapter protein; 36 kDa phospho-tyrosine adaptor protein; 36 kDa phosphotyrosine adapter protein; linker for activation of T cells; linker for activation of T cells, transmembrane adaptor; Linker for activation of T-cells family member 1; p36-38; pp36

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Gene Aliases: LAT; LAT1; pp36

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UniProt ID: (Human) O43561

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Entrez Gene ID: (Human) 27040

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Function(s)

SH3/SH2 adaptor activity Ras guanyl-nucleotide exchange factor activity protein binding protein kinase binding

Process(es)

MAPK cascade adaptive immune response lymphocyte homeostasis inflammatory response immune response integrin-mediated signaling pathway Ras protein signal transduction positive regulation of signal transduction gene expression calcium-mediated signaling platelet activation intracellular signal transduction Fc-epsilon receptor signaling pathway T cell activation mast cell degranulation positive regulation of GTPase activity positive regulation of protein kinase activity T cell receptor signaling pathway regulation of T cell activation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-LAT (Tyr132) Polyclonal Antibody

Phospho-LAT (Tyr132) Antibody (44-224) in WB

Product Details

44-224

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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