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Phospho-LAT (Tyr45) Antibody (PA5-105399) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-LAT (Tyr45) in mouse kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-LAT (Tyr45) polyclonal antibody (Product # PA5-105399) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-105399
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human LAT(Accession O43561), corresponding to amino acid residues around phosphorylated Tyr45.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2816827
Product Specific Information
Antibody detects endogenous levels of LAT only when phosphorylated at Tyr45.
Target Information
LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 36 kDa phospho-tyrosine adapter protein; 36 kDa phospho-tyrosine adaptor protein; 36 kDa phosphotyrosine adapter protein; linker for activation of T cells; linker for activation of T cells, transmembrane adaptor; Linker for activation of T-cells family member 1; linker protein; p36-38; pp36
Gene Aliases: LAT; LAT1; pp36
UniProt ID: (Human) O43561, (Mouse) O54957, (Rat) O70601
Entrez Gene ID: (Human) 27040, (Mouse) 16797, (Rat) 81511
MAPK cascade
adaptive immune response
lymphocyte homeostasis
inflammatory response
immune response
integrin-mediated signaling pathway
Ras protein signal transduction
positive regulation of signal transduction
gene expression
calcium-mediated signaling
platelet activation
intracellular signal transduction
Fc-epsilon receptor signaling pathway
T cell activation
mast cell degranulation
positive regulation of GTPase activity
positive regulation of protein kinase activity
T cell receptor signaling pathway
regulation of T cell activation
immune system process
cellular defense response
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
homeostasis of number of cells
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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