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Phospho-NLRC4 (Ser533) Antibody (PA5-143728) in WB
Western blot of Phospho-NLRC4 (Ser533) in mouse recombinant NLRC4 (Ser533) phosphorylated peptide (lanes 1 & 4) and dephosphorylated peptide (lanes 2 & 5), as well as human PMA-differentiated THP1 cells (lanes 3 & 6). The blots were probed with rabbit anti-NLRC4 (a.a. 525-538) polyclonal (lanes 1-3) and Phospho-NLRC4 (Ser533) polyclonal antibody (Product # PA5-143728) (lanes 4-6).
Product Details
PA5-143728
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Phospho-NLRC4 (Ser-533) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding serine 533 in mouse NLRC4. This sequence has high homology to human and rat NLRC4, and is not homologous to sequences in other NLR family members.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2942956
Product Specific Information
The antibody was cross-adsorbed to unphosphorylated NLRC4 (Ser-533) peptide before affinity purification using phospho-NLRC4 (Ser-533) peptide. This antibody antibody detects a 110 kDa* protein on SDS-PAGE immunoblots of PMA-differentiated THP1 human macrophages. In addition, the antibody detects a mouse recombinant phospho-NLRC4 (Ser-533) peptide, but does not detect this peptide after lambda phosphatase treatment.
Target Information
NLRC4 encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CARD, LRR, and NACHT-containing protein; caspase recruitment domain family, member 12; Caspase recruitment domain-containing protein 12; CED-4-like protein Clan; Ice protease-activating factor; ICE-protease activating factor; Ipaf; NLR family CARD domain-containing protein 4; NLR family, CARD domain containing 4; NOD-like receptor C4; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4
Gene Aliases: 9530011P19Rik; AIFEC; CARD12; CLAN; CLAN1; CLANA; CLANB; CLANC; CLAND; CLR2.1; FCAS4; IPAF; NLRC4; UNQ6189/PRO20215
UniProt ID: (Human) Q9NPP4, (Mouse) Q3UP24, (Rat) F1M649
Entrez Gene ID: (Human) 58484, (Mouse) 268973, (Rat) 298784
activation of innate immune response
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
inflammatory response
regulation of signal transduction
detection of bacterium
protein ubiquitination
defense response to bacterium
regulation of apoptotic process
positive regulation of apoptotic process
innate immune response
interleukin-1 beta secretion
positive regulation of NF-kappaB transcription factor activity
protein homooligomerization
pyroptosis
mitotic spindle assembly
inhibition of cysteine-type endopeptidase activity involved in apoptotic process
immune system process
regulation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of neuron apoptotic process
negative regulation of apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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