Invitrogen

Phospho-Nkx2.1 (Ser23) Polyclonal Antibody

View all (85) Nkx2.1 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-105322

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human NKX2-1(Accession P43699), corresponding to amino acid residues around phosphorylated Ser23.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2816750

Product Specific Information

Antibody detects endogenous levels of TTF1 only when phosphorylated at Ser23.

Target Information

This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Homeobox protein NK-2 homolog A; Homeobox protein Nkx-2.1; NK-2 homolog A; T/EBP; Thyroid nuclear factor 1; Thyroid transcription factor 1; thyroid transcription factor 1 TTF-1 NK-2; Thyroid-specific enhancer-binding protein; tinman; TTF-1; TTF1

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Gene Aliases: AV026640; BCH; BHC; NK-2; Nkx-2.1; NKX2-1; NKX2.1; NKX2A; NMTC1; T/EBP; TEBP; TITF1; TTF-1; TTF1

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UniProt ID: (Human) P43699, (Mouse) P50220

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Entrez Gene ID: (Human) 7080, (Mouse) 21869, (Rat) 25628

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Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II regulatory region DNA binding core promoter binding intronic transcription regulatory region sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding enzyme binding transcription regulatory region DNA binding intronic transcription regulatory region DNA binding transcription factor binding sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding homeodomain transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter neuron migration regulation of blood volume by renin-angiotensin transcription, DNA-templated phospholipid metabolic process axon guidance brain development endoderm development locomotory behavior feeding behavior response to hormone positive regulation of gene expression negative regulation of epithelial to mesenchymal transition globus pallidus development hippocampus development cerebral cortex cell migration forebrain dorsal/ventral pattern formation forebrain neuron fate commitment cerebral cortex GABAergic interneuron differentiation pituitary gland development lung development negative regulation of cell migration negative regulation of transforming growth factor beta receptor signaling pathway thyroid gland development forebrain development developmental induction response to lipopolysaccharide Leydig cell differentiation hyperosmotic salinity response positive regulation of circadian rhythm response to ethanol negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter development of primary female sexual characteristics rhythmic process anatomical structure formation involved in morphogenesis oligodendrocyte differentiation lung saccule development epithelial tube branching involved in lung morphogenesis Clara cell differentiation Type II pneumocyte differentiation regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter pattern specification process organ morphogenesis telencephalon development forebrain neuron differentiation cerebral cortex neuron differentiation telencephalon cell migration menarche neuron fate commitment circadian rhythm
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