Invitrogen

Phospho-Nrf2 (Ser40) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

6 Published Figures

14 References

View all (42) Nrf2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Phospho-Nrf2 (Ser40) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

Phospho-Nrf2 (Ser40) Antibody (PA5-67520) in ICC/IF

Immunofluorescence analysis of phospho-Nuclear factor erythroid 2-related factor 2 (Ser40) was performed using 70% confluent log phase Hep G2 treated with TBHQ (100 micromolar for 4h). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Phospho-Nrf2 (Ser40) P... View More

Phospho-Nrf2 (Ser40) Antibody in Immunohistochemistry (IHC)

Phospho-Nrf2 (Ser40) Antibody in Western Blot (WB)

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Product Details

PA5-67520

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Bovine, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthesized peptide derived from human Nrf2 around the phosphorylation site of S40.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2691678

Product Specific Information

Predicted to react with Mouse and Rat samples.

Target Information

Nfr1 (also designated TCF11), Nrf2 and Nfr3 are bZIP transcription factors that heterodimer with Maf proteins to bind MARE (Maf recognition elements) sequences. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Zinc Finger Protein 90 Knockdown Promotes Cisplatin Sensitivity via Nrf2/HO-1 Pathway in Ovarian Cancer Cell.

Cancers

Wu CH,Feng CW,Wang CL,Wen ZH,Long CY,Tang FH

PA5-67520 was used in Western Blotting to discuss the role of Zfp90 in ovarian cancer (OC) cell lines' sensitivity to cisplatin.

Fri Mar 03 00:00:00 EST 2023

NRF2/ARE mediated antioxidant response to glaucoma: role of glia and retinal ganglion cells.

Acta neuropathologica communications

Naguib S,Backstrom JR,Artis E,Ghose P,Stahl A,Hardin R,Haider AA,Ang J,Calkins DJ,Rex TS

PA5-67520 was used in Immunohistochemistry to provide neuroprotection.

Tue Oct 24 00:00:00 EDT 2023

NRF2/ARE mediated antioxidant response to glaucoma: role of glia and retinal ganglion cells.

Acta neuropathologica communications

Naguib S,Backstrom JR,Artis E,Ghose P,Stahl A,Hardin R,Haider AA,Ang J,Calkins DJ,Rex TS

PA5-67520 was used in Immunohistochemistry to provide neuroprotection.

Tue Oct 24 00:00:00 EDT 2023

Acidosis Activates the Nrf2 Pathway in Renal Proximal Tubule-Derived Cells through a Crosstalk with Renal Fibroblasts.

Antioxidants (Basel, Switzerland)

Schulz MC,Dubourg V,Nolze A,Kopf M,Schwerdt G,Gekle M

PA5-67520 was used in Western Blotting to show that extracellular acidosis activates the cytoprotective transcription factor Nrf2 in renal epithelial cells co-cultivated with renal fibroblasts, thereby enhancing the expression of cytoprotective TKT.

Wed Feb 08 00:00:00 EST 2023

Osthole Antagonizes Microglial Activation in an NRF2-Dependent Manner.

Molecules (Basel, Switzerland)

Liu CH,Chen MY,Kuo YH,Cheng J,Chang LZ,Chang MS,Chuang TN,Hsieh WT,Xiao YR,Wu BT,Lin WY,Liu HP

PA5-67520 was used in Western Blotting to prove that OST antagonizes microglial activation induced by either LPS or Aβ and that NRF2 is necessary for OST's antagonism.

Wed Jan 04 00:00:00 EST 2023

Ixeris dentata and Lactobacillus gasseri media protect against periodontitis through Nrf2-HO-1 signalling pathway.

Scientific reports

Lee HY,Lee GH,Kim JH,Cheng J,Cho JH,Suh JW,Chae HJ

PA5-67520 was used in Western Blotting to demonstrate that IXD/LGM inhibits oxidative stress, periodontal inflammation, and its resultant alveolar bone loss in which Akt (also known as protein kinase B)-nuclear factor-erythroid 2-related factor 2 (Nrf2)-hemoxygenase-1 (HO-1) signaling is involved.

Tue Aug 08 00:00:00 EDT 2023

Rumen-protected methionine during heat stress alters mTOR, insulin signaling, and 1-carbon metabolism protein abundance in liver, and whole-blood transsulfuration pathway genes in Holstein cows.

Journal of dairy science

Coleman DN,Totakul P,Onjai-Uea N,Aboragah A,Jiang Q,Vailati-Riboni M,Pate RT,Luchini D,Paengkoum P,Wanapat M,Cardoso FC,Loor JJ

PA5-67520 was used in Western Blotting to investigate effects of rumen-protected Met (RPM) during a heat stress (HS) challenge on (1) hepatic abundance of mTOR, insulin, and antioxidant signaling proteins, (2) enzymes in 1-carbon metabolism, and (3) innate immunity.

Thu Sep 01 00:00:00 EDT 2022

Deficiency of optineurin enhances osteoclast differentiation by attenuating the NRF2-mediated antioxidant response.

Experimental & molecular medicine

Xue P,Hu X,Chang E,Wang L,Chen M,Wu TH,Lee DJ,Foster BL,Tseng HC,Ko CC

PA5-67520 was used in Western Blot to test the hypothesis that optineurin interacts with nuclear factor erythroid-derived factor 2-related factor 2 (NRF2) to modulate receptor activator of nuclear factor kappa-B ligand induced reactive oxygen species signaling and osteoclastogenesis.

Thu Apr 01 00:00:00 EDT 2021

Retinal oxidative stress activates the NRF2/ARE pathway: An early endogenous protective response to ocular hypertension.

Redox biology

Naguib S,Backstrom JR,Gil M,Calkins DJ,Rex TS

PA5-67520 was used in Western Blot to suggest that exogenous activation of this pathway might further slow glaucomatous neurodegeneration.

Tue Jun 01 00:00:00 EDT 2021

Retinal oxidative stress activates the NRF2/ARE pathway: An early endogenous protective response to ocular hypertension.

Redox biology

Naguib S,Backstrom JR,Gil M,Calkins DJ,Rex TS

PA5-67520 was used in Western Blot to suggest that exogenous activation of this pathway might further slow glaucomatous neurodegeneration.

Tue Jun 01 00:00:00 EDT 2021

Antioxidant activity and laxative effects of tannin-enriched extract of Ecklonia cava in loperamide-induced constipation of SD rats.

PloS one

Kim JE,Choi YJ,Lee SJ,Gong JE,Lee YJ,Sung JE,Jung YS,Lee HS,Hong JT,Hwang DY

PA5-67520 was used in Western Blot to investigate the role of tannin-enriched extracts of Ecklonia cava on the regulation of oxidative balance and laxative activity in chronic constipation.

Tue Aug 03 00:00:00 EDT 2021

Antioxidant activity and laxative effects of tannin-enriched extract of Ecklonia cava in loperamide-induced constipation of SD rats.

PloS one

Kim JE,Choi YJ,Lee SJ,Gong JE,Lee YJ,Sung JE,Jung YS,Lee HS,Hong JT,Hwang DY

PA5-67520 was used in Western Blot to investigate the role of tannin-enriched extracts of Ecklonia cava on the regulation of oxidative balance and laxative activity in chronic constipation.

Tue Aug 03 00:00:00 EDT 2021

α-Synuclein pathology in Parkinson disease activates homeostatic NRF2 anti-oxidant response.

Acta neuropathologica communications

Delaidelli A,Richner M,Jiang L,van der Laan A,Bergholdt Jul Christiansen I,Ferreira N,Nyengaard JR,Vægter CB,Jensen PH,Mackenzie IR,Sorensen PH,Jan A

Published figure using Phospho-Nrf2 (Ser40) polyclonal antibody (Product # PA5-67520) in Immunohistochemistry

Sun Jun 06 00:00:00 EDT 2021

The effects of meldonium on the acute ischemia/reperfusion liver injury in rats.

Scientific reports

Đurašević S,Stojković M,Sopta J,Pavlović S,Borković-Mitić S,Ivanović A,Jasnić N,Tosti T,Đurović S,Đorđević J,Todorović Z

PA5-67520 was used in Western Blotting to investigate the effects of a 4-week meldonium pre-treatment (300 mg/kg b.m./day) on the acute I/R liver injury in Wistar strain male rats.

Thu Jan 14 00:00:00 EST 2021

The effects of meldonium on the acute ischemia/reperfusion liver injury in rats.

Scientific reports

Đurašević S,Stojković M,Sopta J,Pavlović S,Borković-Mitić S,Ivanović A,Jasnić N,Tosti T,Đurović S,Đorđević J,Todorović Z

PA5-67520 was used in Western Blotting to investigate the effects of a 4-week meldonium pre-treatment (300 mg/kg b.m./day) on the acute I/R liver injury in Wistar strain male rats.

Thu Jan 14 00:00:00 EST 2021

Phosphorylation of nuclear factor erythroid 2-like 2 (NFE2L2) in mammary tissue of Holstein cows during the periparturient period is associated with mRNA abundance of antioxidant gene networks.

Journal of dairy science

Han LQ,Zhou Z,Ma Y,Batistel F,Osorio JS,Loor JJ

PA5-67520 was used in Western Blotting to hypothesize that NFE2L2 phosphorylation status and target gene mRNA abundance in the mammary gland of dairy cows is altered around parturition.

Sun Jul 01 00:00:00 EDT 2018

Methionine supply alters mammary gland antioxidant gene networks via phosphorylation of nuclear factor erythroid 2-like 2 (NFE2L2) protein in dairy cows during the periparturient period.

Journal of dairy science

Han L,Batistel F,Ma Y,Alharthi ASM,Parys C,Loor JJ

PA5-67520 was used in Western Blotting to evaluate, in the mammary tissue of periparturient cows, the antioxidant effect of a dietary methionine supply, associated with phosphorylation of NFE2L2.

Sat Sep 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: E4TF1-60; E4TF1A; NF-E2-related factor 2; NFE2-related factor 2; NFT2; nrf2; NRF2A; nuclear factor erythroid 2-like 2; Nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2; nuclear factor, erythroid 2-like 2; Nuclear factor, erythroid derived 2, like 2

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Gene Aliases: AI194320; HEBP1; NFE2L2; NRF2

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UniProt ID: (Human) Q16236, (Mouse) Q60795, (Rat) O54968

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Entrez Gene ID: (Human) 4780, (Mouse) 18024, (Rat) 83619

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Function(s)

transcription regulatory region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II activating transcription factor binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding transcription cofactor binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding protein domain specific binding transcription regulatory region DNA binding sequence-specific DNA binding protein heterodimerization activity basic leucine zipper transcription factor

Process(es)

Performance Guarantee

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Phospho-Nrf2 (Ser40) Polyclonal Antibody

Phospho-Nrf2 (Ser40) Antibody (PA5-67520) in ICC/IF

Product Details

PA5-67520

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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