Invitrogen

Phospho-PAK1/2/3 (Ser144, Ser141, Ser139) Polyclonal Antibody

View all (11) PAK1/2/3 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-101019

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human PAK3(Accession O75914), corresponding to amino acid residues around phosphorylated Ser139.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2815853

Product Specific Information

Antibody detects endogenous levels of PAK1/2/3 only when phosphorylated at PS144+S141+S139.

Target Information

In mammals, there are several identified isoforms of p21-Activated Protein Kinases or PAKs: alpha-PAK (also known as PAK-1) and beta-PAK (also known as PAK-3) are mostly brain-specific, while gamma-PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated wit X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003; Walter et al., 1998). Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: activated protein kinase alpha; ADRB2; adriamycin resistance-associated; Alpha-PAK; Beta-PAK; CDC42/RAC effector kinase PAK-A; CDC42/RAC effector kinase PAK-B; CDKN1A; EC 2.7.11.1; gamma pak; Gamma-PAK; hPAK3; kinase PAK1; Oligophrenin-3; p21 (CDKN1A)-activated kinase 1; p21 (CDKN1A)-activated kinase 2; p21 (CDKN1A)-activated kinase 3; p21 protein (Cdc42/Rac)-activated kinase 1; p21 protein (Cdc42/Rac)-activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 3; p21-activated kinase 1; p21-activated kinase 2; p21-activated kinase 3; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p21/Cdc42/Rac1-activated kinase 3 (yeast Ste20-related); p58; p65-PAK; p68-PAK; PAK 1; PAK alpha; PAK beta; PAK gamma; PAK-1; PAK-2; PAK-3; PAK65; Protein kinase MUK2; S6/H4 kinase; Serine/threonine-protein kinase PAK 1; Serine/threonine-protein kinase PAK 2; Serine/threonine-protein kinase PAK 3; STE20 homolog, yeast

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Gene Aliases: 5330420P17Rik; A130002K10Rik; AI836325; ARA; AW045634; beta-PAK; bPAK; D16Ertd269e; gamma-PAK; mKIAA4182; MRX30; MRX47; OPHN3; PAK-1; PAK-2; PAK-3; PAK1; PAK2; PAK3; PAK3beta; PAK65; Pak65alpha; Pak65beta; Paka; PAKalpha; Pakb; PAKgamma; Stk4

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UniProt ID: (Human) Q13153, (Human) Q13177, (Human) O75914, (Rat) P35465, (Mouse) Q8CIN4, (Rat) Q64303, (Mouse) O88645, (Rat) Q62829

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Entrez Gene ID: (Human) 5058, (Human) 5062, (Human) 5063, (Mouse) 18479, (Rat) 29431, (Mouse) 224105, (Rat) 29432, (Mouse) 18481, (Rat) 29433

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Function(s)
protein kinase activity protein serine/threonine kinase activity receptor signaling protein serine/threonine kinase activity protein binding collagen binding ATP binding protein kinase binding protein tyrosine kinase activator activity identical protein binding cadherin binding involved in cell-cell adhesion MAP kinase kinase activity Rho GTPase binding SH3 domain binding metal ion binding nucleotide binding catalytic activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups Rac GTPase binding MAP kinase kinase kinase activity GTPase binding non-receptor serine/threonine protein kinase
Process(es)
MAPK cascade mitotic cell cycle response to hypoxia positive regulation of protein phosphorylation stimulatory C-type lectin receptor signaling pathway protein phosphorylation exocytosis apoptotic process axonogenesis positive regulation of cell proliferation positive regulation of cell migration T cell costimulation actin cytoskeleton reorganization cellular response to insulin stimulus positive regulation of peptidyl-serine phosphorylation positive regulation of intracellular estrogen receptor signaling pathway Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis wound healing positive regulation of JUN kinase activity protein autophosphorylation ephrin receptor signaling pathway branching morphogenesis of an epithelial tube neuron projection morphogenesis T cell receptor signaling pathway positive regulation of stress fiber assembly negative regulation of cell proliferation involved in contact inhibition negative regulation of protein kinase activity signal transduction viral process phosphorylation peptidyl-serine phosphorylation signal transduction by protein phosphorylation regulation of growth negative regulation of apoptotic process vascular endothelial growth factor receptor signaling pathway regulation of defense response to virus by virus positive regulation of peptidyl-tyrosine phosphorylation dendritic spine development positive regulation of protein tyrosine kinase activity cellular response to organic cyclic compound cell-cell adhesion positive regulation of extrinsic apoptotic signaling pathway negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis activation of MAPK activity dendrite development regulation of actin filament polymerization positive regulation of neuron apoptotic process synapse organization dendritic spine morphogenesis positive regulation of dendritic spine morphogenesis positive regulation of DNA biosynthetic process Rho protein signal transduction regulation of mitotic cell cycle neuromuscular junction development metabolic process response to organic substance regulation of gene expression cell migration amygdala development actin cytoskeleton organization stress-activated protein kinase signaling cascade activation of protein kinase activity regulation of actin cytoskeleton organization regulation of apoptotic process receptor clustering regulation of MAPK cascade gamma-aminobutyric acid secretion, neurotransmission glutamate secretion, neurotransmission observational learning regulation of long-term synaptic potentiation activation of MAPKK activity small GTPase mediated signal transduction intracellular signal transduction multicellular organism development regulation of neuron projection development
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