Invitrogen

Phospho-PERK (Thr982) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

7 Published Figures

10 References

View all (33) PERK antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (7)
Advanced Verification (1)

Phospho-PERK (Thr982) Antibody in Western Blot (WB)

FIGURE: 1 / 8

Phospho-PERK (Thr982) Antibody (PA5-40294) in WB

International journal of molecular sciences 2023 - Figure 2 LHB induces ER stress and promotes tumor formation by regulating the cell cycle. ( A ) The expression of UPR-related proteins in HCC cells after overexpression of LHB was determined by Western blotting. ( B ) The mRNA expression of transcription factors ATF4, XBP1s, and CHOP in HCC cells transfected with LHB was detected by qRT-PCR. ( C ) The cell cycle distribution of LHB stably transfected MHCC-9... View More

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Product Details

PA5-40294

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

1:100-1:300

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthesized peptide derived from human PERK around the phosphorylation site of T982.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2576881

Product Specific Information

This antibody is predicted to react with mouse and rat based on sequence homology.

Target Information

EIF2AK3 (PERK) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses, leading to a decrease in protein sythesis. In the case of EIF2AK3, signaling is initiated by misfolded proteins in the ER. Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS), also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Deficiency of Perry syndrome-associated p150^Glued in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities.

NPJ Parkinson's disease

Yu J,Yang X,Zheng J,Sgobio C,Sun L,Cai H

PA5-40294 was used in Western Blotting to demonstrate the importance of p150Glued in controlling the structure and function of ER, which is critical for the survival and function of midbrain DAergic neurons in PS. © 2023.

Tue Mar 07 00:00:00 EST 2023

Deficiency of Perry syndrome-associated p150^Glued in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities.

NPJ Parkinson's disease

Yu J,Yang X,Zheng J,Sgobio C,Sun L,Cai H

Tue Mar 07 00:00:00 EST 2023

Large HBV Surface Protein-Induced Unfolded Protein Response Dynamically Regulates p27 Degradation in Hepatocellular Carcinoma Progression.

International journal of molecular sciences

Guo Y,Shao J,Zhang R,Han M,Kong L,Liu Z,Li H,Wei D,Lu M,Zhang S,Zhang C,Wei H,Chen Z,Bian H

PA5-40294 was used in Western Blot to identify intensive crosstalk between ER stress and cell cycle progression in HCC.

Thu Sep 07 00:00:00 EDT 2023

Endoplasmic reticulum stress mediates parathyroid hormone-induced apoptosis in vascular smooth muscle cells.

Renal failure

Duan S,Chen X,Liu Y,Guo W,Liu W

PA5-40294 was used in Western Blotting to suggest that ER stress participates in PTH-induced apoptosis of VSMCs, which may be a possible mechanism of PTH-promoted vascular calcification in CKD patients.

Thu Dec 01 00:00:00 EST 2022

Dihydroartemisinin triggers ferroptosis in primary liver cancer cells by promoting and unfolded protein response‑induced upregulation of CHAC1 expression.

Oncology reports

Wang Z,Li M,Liu Y,Qiao Z,Bai T,Yang L,Liu B

PA5-40294 was used in Western Blot to determine whether and how dihydroartemisinin (DHA) could promote the death of primary liver cancer cells by inducing ferroptosis.

Mon Nov 01 00:00:00 EDT 2021

Dihydroartemisinin triggers ferroptosis in primary liver cancer cells by promoting and unfolded protein response‑induced upregulation of CHAC1 expression.

Oncology reports

Wang Z,Li M,Liu Y,Qiao Z,Bai T,Yang L,Liu B

PA5-40294 was used in Western Blot to determine whether and how dihydroartemisinin (DHA) could promote the death of primary liver cancer cells by inducing ferroptosis.

Mon Nov 01 00:00:00 EDT 2021

High doses of dexamethasone induce endoplasmic reticulum stress-mediated apoptosis by promoting calcium ion influx-dependent CHOP expression in osteoblasts.

Molecular biology reports

Guo Y,Hao D,Hu H

Published figure using Phospho-PERK (Thr982) polyclonal antibody (Product # PA5-40294) in Western Blot

Wed Dec 01 00:00:00 EST 2021

Targeted Inhibition of P4HB Promotes Cell Sensitivity to Gemcitabine in Urothelial Carcinoma of the Bladder.

OncoTargets and therapy

Wang X,Bai Y,Zhang F,Yang Y,Feng D,Li A,Yang Z,Li D,Tang Y,Wei X,Wei W,Han P

PA5-40294 was used in Western Blotting to investigate whether the inhibition of P4HB enhances the anticancer efficacy of gemcitabine in BC cells and to study the underlying molecular mechanisms.

Sun Apr 17 00:00:00 EDT 2022

Targeted Inhibition of P4HB Promotes Cell Sensitivity to Gemcitabine in Urothelial Carcinoma of the Bladder.

OncoTargets and therapy

Wang X,Bai Y,Zhang F,Yang Y,Feng D,Li A,Yang Z,Li D,Tang Y,Wei X,Wei W,Han P

PA5-40294 was used in Western Blotting to investigate whether the inhibition of P4HB enhances the anticancer efficacy of gemcitabine in BC cells and to study the underlying molecular mechanisms.

Sun Apr 17 00:00:00 EDT 2022

Cells

Mytych J,Sołek P,Będzińska A,Rusinek K,Warzybok A,Tabęcka-Łonczyńska A,Koziorowski M

PA5-40294 was used in Western Blotting to provide evidence of klotho involvement in the crosstalk on the line ER-Golgi, which may, in turn, affect activation of SASP.

Tue Jan 21 00:00:00 EST 2020

Salubrinal enhances eIF2α phosphorylation and improves fertility in a mouse model of Classic Galactosemia.

Biochimica et biophysica acta. Molecular basis of disease

Balakrishnan B,Siddiqi A,Mella J,Lupo A,Li E,Hollien J,Johnson J,Lai K

PA5-40294 was used in Western Blotting to show that Salubrinal protects against galactosemia-induced primordial follicle loss in a fashion that includes suppressing the de-phosphorylation of eIF2α, and that intervention in this way significantly improves and extends ovarian function, fertility, and fecundity.

Fri Nov 01 00:00:00 EDT 2019

Knockdown of cytokeratin 8 overcomes chemoresistance of chordoma cells by aggravating endoplasmic reticulum stress through PERK/eIF2α arm of unfolded protein response and blocking autophagy.

Cell death & disease

Wang D,Zhang P,Xu X,Wang J,Wang D,Peng P,Zheng C,Meng QJ,Yang L,Luo Z

Published figure using Phospho-PERK (Thr982) polyclonal antibody (Product # PA5-40294) in Western Blot

Mon Nov 25 00:00:00 EST 2019

LACC1 Required for NOD2-Induced, ER Stress-Mediated Innate Immune Outcomes in Human Macrophages and LACC1 Risk Variants Modulate These Outcomes.

Cell reports

Huang C,Hedl M,Ranjan K,Abraham C

PA5-40294 was used in Western Blotting to investigate that, if, upon stimulation of the pattern-recognition receptor (PRR) NOD2, LACC1 localizes to the endoplasmic reticulum (ER) and forms a complex with ER-stress sensors.

Tue Dec 24 00:00:00 EST 2019

Bioinformatics

Protein Aliases: 2.7.11.1; Eukaryotic translation initiation factor 2-alpha kinase 3; HsPEK; OTTHUMP00000207187; OTTHUMP00000207188; Pancreatic eIF2-alpha kinase; PRKR-like endoplasmic reticulum kinase

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Gene Aliases: AI427929; EIF2AK3; PEK; PERK; WRS

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UniProt ID: (Human) Q9NZJ5, (Rat) Q9Z1Z1, (Mouse) Q9Z2B5

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Entrez Gene ID: (Human) 9451, (Rat) 29702, (Mouse) 13666

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Function(s)

protein kinase activity protein serine/threonine kinase activity eukaryotic translation initiation factor 2alpha kinase activity protein binding ATP binding enzyme binding protein phosphatase binding identical protein binding protein homodimerization activity Hsp90 protein binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups eukaryotic initiation factor eIF2 binding non-receptor serine/threonine protein kinase

Process(es)

Performance Guarantee

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Phospho-PERK (Thr982) Polyclonal Antibody

Phospho-PERK (Thr982) Antibody (PA5-40294) in WB

Product Details

PA5-40294

Western Blot (WB)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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