Invitrogen

Phospho-PKA alpha/beta (Thr197) Recombinant Superclonal Antibody (6HCLC)

View all (6) PKA alpha/beta antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

711722

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
-

Immunocytochemistry (ICC/IF)

2 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Clone

6HCLC

Immunogen

Peptide corresponding to Human PRKACA, PRKACB (aa Chemically synthesized phosphopeptide derived from a region of human PKA around 197)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2633194

Product Specific Information

This antibody is predicted to react with Monkey, Rat, Mouse, Bovine and Pig

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

Target Information

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PRKACB is a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cAMP-dependent protein kinase C beta; cAMP-dependent protein kinase catalytic beta subunit isoform 4ab; cAMP-dependent protein kinase catalytic subunit alpha; cAMP-dependent protein kinase catalytic subunit alpha, isoform 1; cAMP-dependent protein kinase catalytic subunit beta; PKA C-alpha; PKA C-beta; protein kinase A catalytic subunit; protein kinase A catalytic subunit beta; protein kinase, cAMP-dependent, alpha catalytic subunit; protein kinase, cAMP-dependent, beta catalytic subunit; protein kinase, cAMP-dependent, catalytic, alpha; protein kinase, cAMP-dependent, catalytic, beta; sperm cAMP-dependent protein kinase catalytic subunit

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Gene Aliases: PKA C-beta; PKACA; PKACB; PKCD; PPNAD4; PRKACA; PRKACB

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UniProt ID: (Human) P17612, (Human) P22694, (Mouse) P05132, (Mouse) P68181

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Entrez Gene ID: (Human) 5566, (Human) 5567, (Mouse) 18747, (Mouse) 18749

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Function(s)
protein serine/threonine kinase activity cAMP-dependent protein kinase activity protein serine/threonine/tyrosine kinase activity protein binding ATP binding protein kinase binding ubiquitin protein ligase binding protein complex binding protein kinase A regulatory subunit binding magnesium ion binding kinase activity nucleotide binding protein kinase activity transferase activity transferase activity, transferring phosphorus-containing groups Rab GTPase binding non-receptor serine/threonine protein kinase
Process(es)
G2/M transition of mitotic cell cycle mesoderm formation neural tube closure regulation of heart rate stimulatory C-type lectin receptor signaling pathway renal water homeostasis mRNA processing protein phosphorylation blood coagulation positive regulation of cell proliferation regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion peptidyl-serine phosphorylation peptidyl-threonine phosphorylation activation of protein kinase A activity calcium-mediated signaling using intracellular calcium source lipoprotein metabolic process regulation of protein binding regulation of cellular respiration regulation of osteoblast differentiation protein autophosphorylation positive regulation of protein export from nucleus sperm capacitation negative regulation of meiotic cell cycle regulation of cytosolic calcium ion concentration regulation of synaptic transmission, glutamatergic regulation of cardiac muscle contraction regulation of ryanodine-sensitive calcium-release channel activity regulation of proteasomal protein catabolic process regulation of protein processing positive regulation of cell cycle arrest cellular response to glucose stimulus cellular response to parathyroid hormone stimulus cellular response to glucagon stimulus cellular response to epinephrine stimulus cell communication by electrical coupling involved in cardiac conduction negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning regulation of cardiac conduction regulation of bicellular tight junction assembly signal transduction adenylate cyclase-modulating G-protein coupled receptor signaling pathway response to clozapine phosphorylation modulation of synaptic transmission
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