Invitrogen

Phospho-PP2A alpha (Tyr307) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

4 Published Figures

7 References

View all (27) PP2A alpha antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Phospho-PP2A alpha (Tyr307) Antibody in Western Blot (WB)

FIGURE: 1 / 8

Phospho-PP2A alpha (Tyr307) Antibody (PA5-36874) in WB

Western blot analysis of Phospho-PP2A alpha (Tyr307) in Lane 1: HEK293T cell treated with EGF (0.1 ng/mL, 30 min), Lane 2: mouse brain tissue lysate, Lane 3: H9C2 cell treated with EGF (0.1 ng/mL, 30 min). Samples were incubated with Phospho-PP2A alpha (Tyr307) polyclonal antibody (Product # PA5-36874) at a dilution of 1:500.

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Product Details

PA5-36874

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 7 publications 7 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide derived from human PP2A-alpha around the phosphorylation site of Tyrosine 307

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2553794

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 38 kDa.

Purity is >95% by SDS-PAGE.

Target Information

In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. The PP2A family comprises subfamily members PP2A alpha and PP2A beta. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nonpeptidic Oxazole-Based Prolyl Oligopeptidase Ligands with Disease-Modifying Effects on α-Synuclein Mouse Models of Parkinson's Disease.

Journal of medicinal chemistry

Kilpeläinen TP,Pätsi HT,Svarcbahs R,Julku UH,Eteläinen TS,Cui H,Auno S,Sipari N,Norrbacka S,Leino TO,Jäntti M,Myöhänen TT,Wallén EAA

Published figure using Phospho-PP2A alpha (Tyr307) polyclonal antibody (Product # PA5-36874) in Western Blot

Thu Jun 08 00:00:00 EDT 2023

Prolyl oligopeptidase inhibition reduces oxidative stress via reducing NADPH oxidase activity by activating protein phosphatase 2A.

Free radical biology & medicine

Eteläinen T,Kulmala V,Svarcbahs R,Jäntti M,Myöhänen TT

PA5-36874 was used in Western Blot to suggest that PREP inhibition could also provide neuroprotection by reducing OS, thus broadening the scope of its beneficial effects on neurodegeneration.

Tue Jun 01 00:00:00 EDT 2021

Prolyl oligopeptidase inhibition reduces alpha-synuclein aggregation in a cellular model of multiple system atrophy.

Journal of cellular and molecular medicine

Cui H,Kilpeläinen T,Zouzoula L,Auno S,Trontti K,Kurvonen S,Norrbacka S,Hovatta I,Jensen PH,Myöhänen TT

Published figure using Phospho-PP2A alpha (Tyr307) polyclonal antibody (Product # PA5-36874) in Western Blot

Fri Oct 01 00:00:00 EDT 2021

The Protective Role of Prolyl Oligopeptidase (POP) Inhibition in Kidney Injury Induced by Renal Ischemia-Reperfusion.

International journal of molecular sciences

Casili G,Ardizzone A,Basilotta R,Lanza M,Filippone A,Paterniti I,Esposito E,Campolo M

PA5-36874 was used in Western Blot to investigate the role of POP in kidney ischemia/reperfusion (KI/R).

Tue Nov 02 00:00:00 EDT 2021

The Protective Role of Prolyl Oligopeptidase (POP) Inhibition in Kidney Injury Induced by Renal Ischemia-Reperfusion.

International journal of molecular sciences

Casili G,Ardizzone A,Basilotta R,Lanza M,Filippone A,Paterniti I,Esposito E,Campolo M

PA5-36874 was used in Western Blot to investigate the role of POP in kidney ischemia/reperfusion (KI/R).

Tue Nov 02 00:00:00 EDT 2021

Salicylic Acid Targets Protein Phosphatase 2A to Attenuate Growth in Plants.

Current biology : CB

Tan S,Abas M,Verstraeten I,Glanc M,Molnár G,Hajný J,Lasák P,Petřík I,Russinova E,Petrášek J,Novák O,Pospíšil J,Friml J

Published figure using Phospho-PP2A alpha (Tyr307) polyclonal antibody (Product # PA5-36874) in Western Blot

Mon Feb 03 00:00:00 EST 2020

Prolyl oligopeptidase inhibition activates autophagy via protein phosphatase 2A.

Pharmacological research

Svarcbahs R,Jäntti M,Kilpeläinen T,Julku UH,Urvas L,Kivioja S,Norrbacka S,Myöhänen TT

PA5-36874 was used in Western Blot to demonstrate that PREP regulates autophagy by interacting with protein phosphatase 2A (PP2A) and its endogenous inhibitor, protein phosphatase methylesterase 1 (PME1), and activator (protein phosphatase 2 phosphatase activator, PTPA), thus adjusting its activity and the levels of PP2A in the intracellular pool.

Wed Jan 01 00:00:00 EST 2020

PP2A_C Phospho-Tyr³⁰⁷ Antibodies Are Not Specific for this Modification but Are Sensitive to Other PP2A_C Modifications Including Leu³⁰⁹ Methylation.

Cell reports

Frohner IE,Mudrak I,Schüchner S,Anrather D,Hartl M,Sontag JM,Sontag E,Wadzinski BE,Preglej T,Ellmeier W,Ogris E

PA5-36874 was used in Western Blotting to show that the most cited pTyr307 monoclonal antibodies, E155 and F-8, are not specific for phosphorylated Tyr307 but instead are hampered by PP2AC methylation at leucine 309 or phosphorylation at threonine 304.

Tue Mar 03 00:00:00 EST 2020

Bioinformatics

Protein Aliases: B55-GAMMA; IMYPNO; Methyl-PPP2CA; MGC33570; PP2A alpha; PP2A C terminal; PP2A L9; PP2A Leucine 9; PP2A-alpha; PR52; PR55G; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2a, catalytic subunit, alpha isoform; protein phosphatase-2A-alpha; Replication protein C; RP-C; serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

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Gene Aliases: PP2A; Pp2a1; PP2Ac; PP2CA; PP2Calpha; PPP2CA; R75353; RP-C

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UniProt ID: (Human) P67775, (Mouse) P63330, (Rat) P63331

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Entrez Gene ID: (Human) 5515, (Mouse) 19052, (Rat) 24672

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Function(s)

phosphoprotein phosphatase activity protein binding protein C-terminus binding metal ion binding GABA receptor binding protein serine/threonine phosphatase activity hydrolase activity enzyme binding protein kinase binding protein phosphatase binding protein domain specific binding protein complex binding protein kinase B binding ion channel binding protein dimerization activity tau protein binding protein phosphatase 2A binding protein antigen binding protein heterodimerization activity protein phosphatase

Process(es)

nuclear-transcribed mRNA catabolic process, nonsense-mediated decay inactivation of MAPK activity regulation of DNA replication regulation of transcription, DNA-templated protein dephosphorylation ceramide metabolic process apoptotic process mitotic nuclear envelope reassembly mesoderm development RNA splicing response to organic substance negative regulation of epithelial to mesenchymal transition second-messenger-mediated signaling regulation of Wnt signaling pathway regulation of cell adhesion negative regulation of cell growth regulation of growth negative regulation of tyrosine phosphorylation of Stat3 protein regulation of cell differentiation meiotic cell cycle positive regulation of protein serine/threonine kinase activity negative regulation of transcription from RNA polymerase II promoter regulation of protein phosphorylation negative regulation of protein phosphorylation regulation of receptor activity regulation of protein autophosphorylation positive regulation of protein phosphatase type 2A activity positive regulation of protein dephosphorylation regulation of protein catabolic process negative regulation of transcription factor import into nucleus positive regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of cell cycle protein heterotrimerization cellular response to glucose stimulus negative regulation of calcium ion transmembrane transporter activity cardiac ventricle development heart development cellular response to insulin stimulus cellular response to calcium ion cellular response to cytokine stimulus cellular response to ethanol cellular response to follicle-stimulating hormone stimulus cellular response to steroid hormone stimulus

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-PP2A alpha (Tyr307) Polyclonal Antibody

Phospho-PP2A alpha (Tyr307) Antibody (PA5-36874) in WB

Product Details

PA5-36874

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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