Invitrogen

Phospho-PYK2 (Tyr402) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

24 Published Figures

31 References

View all (41) PYK2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Phospho-PYK2 (Tyr402) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 33

Phospho-PYK2 (Tyr402) Antibody (44-618G) in ICC/IF

Immunofluorescence analysis of PYK2 (pY402) was done on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with PYK2 (pY402) Rabbit Polyclonal Antibody (Product # 44-618G) at 1:250 dilution in 1% BSA and incubated for 3 hours at ... View More

Phospho-PYK2 (Tyr402) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-PYK2 (Tyr402) Antibody in Western Blot (WB)

Phospho-PYK2 (Tyr402) Antibody in Western Blot, Immunohistochemistry (WB, IHC)

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Product Details

44-618G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 23 publications 23 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

Immunocytochemistry (ICC/IF)

1-2 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Rat

Published species

Human, Mouse, Rat, Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Pyk2 that contains tyrosine 402. The sequence is conserved in rat.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533697

Product Specific Information

This product contains enough material for 10 mini-blots. Antibody has been negatively preadsorbed using a nonphosphopeptide then purified by epitope-specific affinity chromatography.

The antibody has been used in western blotting and immunohistochemistry. Other applications may work but have not been tested. Positive controls used were CEF cell extracts +/- Pyk2 transfection or A431 whole cell lysate.

Target Information

PTK2B (FAK2) is a cytoplasmic tyrosine kinase involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. PTK2B regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading, and bone remodeling. FAK2 plays a role in the regulation of the humoral immune response and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. It functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors, cytokine, chemokine, and growth factor receptors, and mediates responses to cellular stress. Aberrant FAK2 expression may play a role in cancer cell proliferation, migration, and invasion in tumor formation and metastasis. Elevated expression has been seen in gliomas, hepatocellular carcinoma, lung cancer, and breast cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PYK2 senses calcium through a disordered dimerization and calmodulin-binding element.

Communications biology

Momin AA,Mendes T,Barthe P,Faure C,Hong S,Yu P,Kadaré G,Jaremko M,Girault JA,Jaremko Ł,Arold ST

44-618G was used in Western Blotting to investigate the mechanism by which the protein tyrosine kinase 2-beta (PYK2) functions as a sensor and effector of cellular calcium influx.

Tue Aug 09 00:00:00 EDT 2022

A novel DDR1 inhibitor enhances the anticancer activity of gemcitabine in pancreatic cancer.

American journal of cancer research

Ko S,Jung KH,Yoon YC,Han BS,Park MS,Lee YJ,Kim SE,Cho YJ,Lee P,Lim JH,Ryu JK,Kim K,Kim TY,Hong S,Lee SH,Hong SS

44-618G was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to demonstrate that KI-301690 can inhibit aberrant ECM expression by DDR1/PYK2/FAK signaling pathway blockade and attenuation of ECM-induced chemoresistance observed in desmoplastic pancreatic tumors, resulting in enhanced antitumor effect through effective induction of gemcitabine apoptosis.

Fri Oct 14 00:00:00 EDT 2022

BDNF increases synaptic NMDA receptor abundance by enhancing the local translation of Pyk2 in cultured hippocampal neurons.

Science signaling

Afonso P,De Luca P,Carvalho RS,Cortes L,Pinheiro P,Oliveiros B,Almeida RD,Mele M,Duarte CB

44-618G was used in Western Blotting to investigate the mechanisms that mediate BDNF-induced alterations in the synaptic proteome that are coupled to synaptic strengthening.

Tue Jun 18 00:00:00 EDT 2019

PYK2 Is Involved in Premalignant Acinar Cell Reprogramming and Pancreatic Ductal Adenocarcinoma Maintenance by Phosphorylating β-Catenin^Y654.

Cellular and molecular gastroenterology and hepatology

Gao C,Chen G,Zhang DH,Zhang J,Kuan SF,Hu W,Esni F,Gao X,Guan JL,Chu E,Hu J

44-618G was used in Western Blotting to investigate the role of proline-rich tyrosine kinase 2 in mutant Kras-induced and pancreatitis-associated acinar-to-ductal metaplasia and PanIN formation, as well as in pancreatic ductal adenocarcinoma maintenance.

Mon Jul 20 00:00:00 EDT 2020

PYK2 Is Involved in Premalignant Acinar Cell Reprogramming and Pancreatic Ductal Adenocarcinoma Maintenance by Phosphorylating β-Catenin^Y654.

Cellular and molecular gastroenterology and hepatology

Gao C,Chen G,Zhang DH,Zhang J,Kuan SF,Hu W,Esni F,Gao X,Guan JL,Chu E,Hu J

Mon Jul 20 00:00:00 EDT 2020

The human ion channel TRPM2 modulates neuroblastoma cell survival and mitochondrial function through Pyk2, CREB, and MCU activation.

American journal of physiology. Cell physiology

Hirschler-Laszkiewicz I,Chen SJ,Bao L,Wang J,Zhang XQ,Shanmughapriya S,Keefer K,Madesh M,Cheung JY,Miller BA

Published figure using Phospho-PYK2 (Tyr402) polyclonal antibody (Product # 44-618G) in Western Blot

Mon Oct 01 00:00:00 EDT 2018

Atypical Endocannabinoid Signaling Initiates a New Form of Memory-Related Plasticity at a Cortical Input to Hippocampus.

Cerebral cortex (New York, N.Y. : 1991)

Wang W,Jia Y,Pham DT,Palmer LC,Jung KM,Cox CD,Rumbaugh G,Piomelli D,Gall CM,Lynch G

44-618G was used in Immunohistochemistry-immunofluorescence to describe the endocannabinoid signalling pathway that stimulates memory related plasticity in the cortex.

Sun Jul 01 00:00:00 EDT 2018

Atypical Endocannabinoid Signaling Initiates a New Form of Memory-Related Plasticity at a Cortical Input to Hippocampus.

Cerebral cortex (New York, N.Y. : 1991)

Wang W,Jia Y,Pham DT,Palmer LC,Jung KM,Cox CD,Rumbaugh G,Piomelli D,Gall CM,Lynch G

44-618G was used in Immunohistochemistry-immunofluorescence to describe the endocannabinoid signalling pathway that stimulates memory related plasticity in the cortex.

Sun Jul 01 00:00:00 EDT 2018

Adhesion structures in leukemia cells and their regulation by Src family kinases.

Cell adhesion & migration

Röselová P,Obr A,Holoubek A,Grebeňová D,Kuželová K

44-618G was used in Western Blotting to analyse the interaction of leukaemia cells with fibronectin.

Fri May 04 00:00:00 EDT 2018

Pyk2 inhibition promotes contractile differentiation in arterial smooth muscle.

Journal of cellular physiology

Grossi M,Bhattachariya A,Nordström I,Turczyńska KM,Svensson D,Albinsson S,Nilsson BO,Hellstrand P

44618G was used in western blot to assess the role of Pyk2 in phenotype modulation in rat carotid artery smooth muscle cells and in cultured intact arteries

Wed Nov 01 00:00:00 EDT 2017

Pyk2 is essential for astrocytes mobility following brain lesion.

Glia

Giralt A,Coura R,Girault JA

Published figure using Phospho-PYK2 (Tyr402) polyclonal antibody (Product # 44-618G) in Western Blot

Fri Apr 01 00:00:00 EDT 2016

A role for the tyrosine kinase Pyk2 in depolarization-induced contraction of vascular smooth muscle.

The Journal of biological chemistry

Mills RD,Mita M,Nakagawa J,Shoji M,Sutherland C,Walsh MP

Published figure using Phospho-PYK2 (Tyr402) polyclonal antibody (Product # 44-618G) in Western Blot

Fri Apr 03 00:00:00 EDT 2015

CD28 and CD3 have complementary roles in T-cell traction forces.

Proceedings of the National Academy of Sciences of the United States of America

Bashour KT,Gondarenko A,Chen H,Shen K,Liu X,Huse M,Hone JC,Kam LC

44-618G was used in immunocytochemistry to investigate the effect of mechanical force on the T cell receptor.

Tue Feb 11 00:00:00 EST 2014

PYK2 selectively mediates signals for growth versus differentiation in response to stretch of spontaneously active vascular smooth muscle.

Physiological reports

Bhattachariya A,Turczyńska KM,Grossi M,Nordström I,Buckbinder L,Albinsson S,Hellstrand P

44-618G was used in western blot to examine the role of the calcium- and integrin-activated proline-rich tyrosine kinase 2 in stretch-induced responses of the rat portal vein.

Wed Jul 16 00:00:00 EDT 2014

Biophysical stimulation induces demyelination via an integrin-dependent mechanism.

Annals of neurology

Lin MY,Frieboes LS,Forootan M,Palispis WA,Mozaffar T,Jafari M,Steward O,Gall CM,Gupta R

Sun Jul 01 00:00:00 EDT 2012

Biophysical stimulation induces demyelination via an integrin-dependent mechanism.

Annals of neurology

Lin MY,Frieboes LS,Forootan M,Palispis WA,Mozaffar T,Jafari M,Steward O,Gall CM,Gupta R

Sun Jul 01 00:00:00 EDT 2012

Biophysical stimulation induces demyelination via an integrin-dependent mechanism.

Annals of neurology

Lin MY,Frieboes LS,Forootan M,Palispis WA,Mozaffar T,Jafari M,Steward O,Gall CM,Gupta R

Sun Jul 01 00:00:00 EDT 2012

Integrin dynamics produce a delayed stage of long-term potentiation and memory consolidation.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Babayan AH,Kramár EA,Barrett RM,Jafari M,Häettig J,Chen LY,Rex CS,Lauterborn JC,Wood MA,Gall CM,Lynch G

44-618G was used in immunohistochemistry to study the role of beta integrin in long-term potentiation and the consolidation of memory.

Wed Sep 12 00:00:00 EDT 2012

Knock-in mutation reveals an essential role for focal adhesion kinase activity in blood vessel morphogenesis and cell motility-polarity but not cell proliferation.

The Journal of biological chemistry

Lim ST,Chen XL,Tomar A,Miller NL,Yoo J,Schlaepfer DD

44-618G was used in western blot to determine the role of FAK activity during development

Fri Jul 09 00:00:00 EDT 2010

Knock-in mutation reveals an essential role for focal adhesion kinase activity in blood vessel morphogenesis and cell motility-polarity but not cell proliferation.

The Journal of biological chemistry

Lim ST,Chen XL,Tomar A,Miller NL,Yoo J,Schlaepfer DD

44-618G was used in western blot to determine the role of FAK activity during development

Fri Jul 09 00:00:00 EDT 2010

The role of proline-rich protein tyrosine kinase 2 in differentiation-dependent signaling in human epidermal keratinocytes.

The Journal of investigative dermatology

Schindler EM,Baumgartner M,Gribben EM,Li L,Efimova T

Tue May 01 00:00:00 EDT 2007

ICAM-1-mediated, Src- and Pyk2-dependent vascular endothelial cadherin tyrosine phosphorylation is required for leukocyte transendothelial migration.

Journal of immunology (Baltimore, Md. : 1950)

Allingham MJ,van Buul JD,Burridge K

Sat Sep 15 00:00:00 EDT 2007

ICAM-1-mediated, Src- and Pyk2-dependent vascular endothelial cadherin tyrosine phosphorylation is required for leukocyte transendothelial migration.

Journal of immunology (Baltimore, Md. : 1950)

Allingham MJ,van Buul JD,Burridge K

Sat Sep 15 00:00:00 EDT 2007

Activation of FAK is necessary for the osteogenic differentiation of human mesenchymal stem cells on laminin-5.

Journal of cellular biochemistry

Salasznyk RM,Klees RF,Boskey A,Plopper GE

44-618G was used in western blot to report that FAK is an important mediator of laminin-5-induced osteogenic differentiation of human mesenchymal stem cells

Thu Feb 01 00:00:00 EST 2007

Focal adhesion kinase signaling pathways regulate the osteogenic differentiation of human mesenchymal stem cells.

Experimental cell research

Salasznyk RM,Klees RF,Williams WA,Boskey A,Plopper GE

44-618G was used in western blot to investigate FAK signaling in the regulation of extracellular matrix-induced osteogenic differentiation of human mesenchymal stem cells

Mon Jan 01 00:00:00 EST 2007

Analyzing FAK and Pyk2 in early integrin signaling events.

Current protocols in cell biology

Bernard-Trifilo JA,Lim ST,Hou S,Schlaepfer DD,Ilic D

Sat Apr 01 00:00:00 EST 2006

Analyzing FAK and Pyk2 in early integrin signaling events.

Current protocols in cell biology

Bernard-Trifilo JA,Lim ST,Hou S,Schlaepfer DD,Ilic D

Sat Apr 01 00:00:00 EST 2006

PYK2 regulates SERCA2 gene expression in neonatal rat ventricular myocytes.

American journal of physiology. Cell physiology

Heidkamp MC,Scully BT,Vijayan K,Engman SJ,Szotek EL,Samarel AM

Mon Aug 01 00:00:00 EDT 2005

Role of PYK2 in the development of obesity and insulin resistance.

Biochemical and biophysical research communications

Yu Y,Ross SA,Halseth AE,Hollenbach PW,Hill RJ,Gulve EA,Bond BR

Fri Sep 09 00:00:00 EDT 2005

Focal adhesion kinase plays a pivotal role in herpes simplex virus entry.

The Journal of biological chemistry

Cheshenko N,Liu W,Satlin LM,Herold BC

Fri Sep 02 00:00:00 EDT 2005

Proline-rich tyrosine kinase 2 (Pyk2) mediates vascular endothelial-cadherin-based cell-cell adhesion by regulating beta-catenin tyrosine phosphorylation.

The Journal of biological chemistry

van Buul JD,Anthony EC,Fernandez-Borja M,Burridge K,Hordijk PL

Fri Jun 03 00:00:00 EDT 2005

Proline-rich tyrosine kinase 2 (Pyk2) mediates vascular endothelial-cadherin-based cell-cell adhesion by regulating beta-catenin tyrosine phosphorylation.

The Journal of biological chemistry

van Buul JD,Anthony EC,Fernandez-Borja M,Burridge K,Hordijk PL

Fri Jun 03 00:00:00 EDT 2005

Salicylate Inhibits Phosphorylation of the Nonreceptor Tyrosine Kinases, Proline-Rich Tyrosine Kinase 2 and c-Src.

Hypertension (Dallas, Tex. : 1979)

Wang Z,Brecher P

Mon Jan 01 00:00:00 EST 2001

Glucose activates protein kinase C-zeta /lambda through proline-rich tyrosine kinase-2, extracellular signal-regulated kinase, and phospholipase D: a novel mechanism for activating glucose transporter translocation.

The Journal of biological chemistry

Bandyopadhyay G,Sajan MP,Kanoh Y,Standaert ML,Quon MJ,Reed BC,Dikic I,Farese RV

Fri Sep 21 00:00:00 EDT 2001

Bioinformatics

Protein Aliases: CADTK; CAK beta; CAK-beta; Calcium-dependent tyrosine kinase; Calcium-regulated non-receptor proline-rich tyrosine kinase; Cell adhesion kinase beta; cellular adhesion kinase beta; FADK; FADK 2; FAK; FAK1; Focal adhesion kinase 2; Proline-rich tyrosine kinase 2; protein kinase B; protein tyrosine kinase 2 beta; Protein-tyrosine kinase 2-beta; PTK2 protein tyrosine kinase 2 beta; PTK2B protein tyrosine kinase 2 beta; RAFTK; Related adhesion focal tyrosine kinase

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Gene Aliases: CADTK; CAKB; CAKbeta; E430023O05Rik; FADK2; FAK2; PKB; PTK; PTK2B; PYK2; RAFTK

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UniProt ID: (Human) Q14289, (Rat) P70600, (Mouse) Q9QVP9

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Entrez Gene ID: (Human) 2185, (Chicken) 421998, (Rat) 50646, (Mouse) 19229

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Function(s)

calmodulin-dependent protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity signal transducer activity NMDA glutamate receptor activity receptor binding protein binding ATP binding protein complex binding 3-phosphoinositide-dependent protein kinase binding protein kinase activity nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-PYK2 (Tyr402) Polyclonal Antibody

Phospho-PYK2 (Tyr402) Antibody (44-618G) in ICC/IF

Product Details

44-618G

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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