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Invitrogen
Phospho-Phospholamban (Ser16, Thr17) Polyclonal Antibody
FIGURE: 1 / 3
Phospho-Phospholamban (Ser16, Thr17) Antibody (PA5-114620) in ICC/IF
Immunocytochemistry analysis of Phospho-Phospholamban (Ser16, Thr17) in HeLa cells (4h of LPS treatment). Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114620) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-... View More
Product Details
PA5-114620
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PLN(Accession P26678), corresponding to amino acid residues around phosphorylated Ser16.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2899256
Product Specific Information
Antibody detects endogenous levels of Phospholamban only when phosphorylated at Ser16/Thr17.
Target Information
Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cardiac CMH18; Cardiac phospholamban; PLB
Gene Aliases: CMD1P; CMH18; PLB; Plm; PLN
UniProt ID: (Human) P26678, (Rat) P61016, (Mouse) P61014
Entrez Gene ID: (Human) 5350, (Rat) 64672, (Mouse) 18821
regulation of the force of heart contraction
calcium ion transport
cellular calcium ion homeostasis
regulation of heart contraction
response to zinc ion
negative regulation of heart rate
regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
negative regulation of ATPase activity
response to insulin
response to testosterone
negative regulation of heart contraction
cardiac muscle tissue development
protein homooligomerization
regulation of calcium ion transport
negative regulation of calcium ion transport
regulation of ryanodine-sensitive calcium-release channel activity
regulation of cardiac muscle cell contraction
adrenergic receptor signaling pathway involved in heart process
regulation of the force of heart contraction by cardiac conduction
regulation of calcium ion import
negative regulation of calcium ion transmembrane transporter activity
regulation of relaxation of muscle
negative regulation of calcium ion binding
regulation of calcium-transporting ATPase activity
negative regulation of calcium-transporting ATPase activity
Notch signaling pathway
negative regulation of catalytic activity
blood circulation
regulation of cytosolic calcium ion concentration
relaxation of cardiac muscle
regulation of cardiac muscle cell membrane potential
negative regulation of calcium ion import
regulation of relaxation of cardiac muscle
negative regulation of calcium ion import into sarcoplasmic reticulum
regulation of cardiac conduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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