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Invitrogen
Phospho-Progesterone Receptor (Ser190) Monoclonal Antibody (1154)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 8
Phospho-Progesterone Receptor (Ser190) Antibody (MA1-413) in ICC/IF
Immunofluorescent analysis of Phospho-Progesterone Receptor pSer190 in untreated T47D cells (left panel) or stimulated cells with 100 nM of Progesterone (right panel). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospho-Progesterone Receptor pSer190 Monclona... View More
Product Details
MA1-413
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1154
Immunogen
Synthetic phosphopeptide corresponding to residues V(184) L P R G L S(p) P A R Q L L(196) of human Progesterone Receptor.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
purified
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_325315
Product Specific Information
MA1-413 detects phospho-progesterone receptor (pSer190) from human samples.
MA1-413 has been successfully used in Western blot, immunohistochemistry, ELISA, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody detects ~81 and ~116 kDa proteins representing phospho-progesterone receptor (pSer190) A and B from T47D cell and human breast carcinoma extracts. Immunohistochemical staining of phospho-progesterone receptor (pSer190) (paraffin samples) can be performed using MA1-413.
The MA1-413 immunogen is a synthetic peptide corresponding to residues V(184) L P R G L S(p) P A R Q L L(196) of human Progesterone Receptor.
Target Information
The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Nuclear receptor subfamily 3 group C member 3; PR; Progesterone receptor
Gene Aliases: NR3C3; PGR; PR
UniProt ID: (Human) P06401
Entrez Gene ID: (Human) 5241
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
steroid hormone receptor activity
steroid binding
protein binding
zinc ion binding
enzyme binding
ATPase binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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