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Invitrogen
Phospho-RNA pol II CTD (Ser5) Recombinant Rabbit Monoclonal Antibody (JM51-21)
FIGURE: 1 / 11
Phospho-RNA pol II CTD (Ser5) Antibody (MA5-32638) in ICC/IF
Immunofluorescence analysis of Phospho-RNA pol II CTD (Ser5) Recombinant Rabbit Monoclonal Antibody (JM51-21) was performed using 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Phospho-RNA pol II CTD (Ser5) Recombi... View More
Product Details
MA5-32638
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JM51-21
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser5 of Human POLR2A aa 1590-1627
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809915
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3'-5' exoribonuclease; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; EC 2.7.7.6; MGC75453; Pol II S2p; Polymerase (RNA II (DNA directed), large polypeptide; polymerase (RNA) II (DNA directed) polypeptide A; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; POLYMERASE II; RNA; RNA POL2; RNA polymerase II 1; RNA polymerase II C-terminal domain; RNA polymerase II carboxy-terminal domain; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RP02; RPB220; RPO21; SUA8
Gene Aliases: 220kDa; hRPB220; hsRPB1; POLR2; POLR2A; POLRA; RPB1; RPBh1; Rpii215; RpIILS; RPO2; Rpo2-1; RPOL2
UniProt ID: (Human) P24928, (Mouse) P08775
Entrez Gene ID: (Human) 5430, (Mouse) 20020, (Rat) 363633
RNA polymerase II activity
DNA binding
DNA-directed RNA polymerase activity
RNA-directed RNA polymerase activity
protein binding
ubiquitin protein ligase binding
poly(A) RNA binding
metal ion binding
core promoter binding
transferase activity
nucleotidyltransferase activity
molecular_function
DNA-directed RNA polymerase
mRNA splicing, via spliceosome
transcription, RNA-templated
transcription-coupled nucleotide-excision repair
DNA-templated transcription, termination
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
7-methylguanosine mRNA capping
fibroblast growth factor receptor signaling pathway
gene expression
gene silencing by RNA
positive regulation of RNA splicing
somatic stem cell population maintenance
snRNA transcription from RNA polymerase II promoter
positive regulation of viral transcription
transcription, DNA-templated
biological_process
response to organic cyclic compound
cellular response to oxygen levels
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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