Invitrogen

Phospho-SGK1 (Thr256) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

4 Published Figures

8 References

View all (27) SGK1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Phospho-SGK1 (Thr256) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 10

Phospho-SGK1 (Thr256) Antibody (44-1260G) in IHC (P)

Immunohistochemistry analysis of Phospho SGK1 (pT256) showing staining in the membrane and weakly in the cytoplasm of paraffin-embedded human hepatocarcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in ... View More

Phospho-SGK1 (Thr256) Antibody in Western Blot (WB)

Phospho-SGK1 (Thr256) Antibody in Immunohistochemistry (IHC)

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Product Details

44-1260G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human SGK1 that contains threonine 256.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533592

Target Information

SGK (SGK1) is a serine/threonine kinase that may be a transcriptional target for p38/MAPK in hepatoma cells. SGK plays an important role in cellular stress response and cell survival. SGK1 is transcriptionally activated by numerous stimuli, including serum, glucocorticoids, osmotic stress, and hormones. It phosphorylates and negatively regulates pro-apoptotic FOXO3A. SGK1 is thought to play an essential function in nephropathy and cardiovascular development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Loss of Serum Glucocorticoid-Inducible Kinase 1 SGK1 Worsens Malabsorption and Diarrhea in Microvillus Inclusion Disease (MVID).

Journal of clinical medicine

Ahsan MK,Dos Reis DC,Barbieri A,Sumigray KD,Nottoli T,Salas PJ,Ameen NA

44-1260G was used in Western Blotting and Immunohistochemistry to conclude that loss of SGK1 worsens carbohydrate malabsorption and diarrhea in MVID.

Tue Jul 19 00:00:00 EDT 2022

Loss of Serum Glucocorticoid-Inducible Kinase 1 SGK1 Worsens Malabsorption and Diarrhea in Microvillus Inclusion Disease (MVID).

Journal of clinical medicine

Ahsan MK,Dos Reis DC,Barbieri A,Sumigray KD,Nottoli T,Salas PJ,Ameen NA

44-1260G was used in Western Blotting and Immunohistochemistry to conclude that loss of SGK1 worsens carbohydrate malabsorption and diarrhea in MVID.

Tue Jul 19 00:00:00 EDT 2022

Aldosterone-stimulated endothelial epithelial sodium channel (EnNaC) plays a role in cold exposure-induced hypertension in rats.

Frontiers in pharmacology

Tang LL,Yang X,Yu SQ,Qin Q,Xue R,Sun Y,Xiao H,Shang AQ,Liu JQ,Han SQ,Liang C,Lou J,Wang QS,Yu CJ,Wu MM,Zhang ZR

44-1260G was used in Western Blotting to investigate whether mesenteric artery (MA) EnNaC is involved in cold exposure-induced hypertension (CIH) and identify the underlying mechanisms in SD rats.

Tue Oct 25 00:00:00 EDT 2022

Cutaneous and hepatic vascular lesions due to a recurrent somatic GJA4 mutation reveal a pathway for vascular malformation.

HGG advances

Ugwu N,Atzmony L,Ellis KT,Panse G,Jain D,Ko CJ,Nassiri N,Choate KA

44-1260G was used in Western Blotting to examine lesions in both hepatic hemangiomas and cutaneous venous malformations to identify genetic drivers.

Thu Apr 08 00:00:00 EDT 2021

Cutaneous and hepatic vascular lesions due to a recurrent somatic GJA4 mutation reveal a pathway for vascular malformation.

HGG advances

Ugwu N,Atzmony L,Ellis KT,Panse G,Jain D,Ko CJ,Nassiri N,Choate KA

44-1260G was used in Western Blotting to examine lesions in both hepatic hemangiomas and cutaneous venous malformations to identify genetic drivers.

Thu Apr 08 00:00:00 EDT 2021

The role of epithelial progesterone receptor isoforms in embryo implantation.

iScience

Li R,Wang X,Huang Z,Balaji J,Kim TH,Wang T,Zhou L,Deleon A,Cook ME,Marbrey MW,Wu SP,Jeong JW,Arora R,DeMayo FJ

44-1260G was used in Immunohistochemistry (Paraffin), Western Blot to demonstrate that PGRA and PGRB in the uterine epithelium act on a similar set of target genes and commonly regulate the LIF-SGK1-FOXO1 signaling pathway for embryo implantation.

Fri Dec 17 00:00:00 EST 2021

The role of epithelial progesterone receptor isoforms in embryo implantation.

iScience

Li R,Wang X,Huang Z,Balaji J,Kim TH,Wang T,Zhou L,Deleon A,Cook ME,Marbrey MW,Wu SP,Jeong JW,Arora R,DeMayo FJ

Fri Dec 17 00:00:00 EST 2021

Stimulation of Epithelial Sodium Channels in Endothelial Cells by Bone Morphogenetic Protein-4 Contributes to Salt-Sensitive Hypertension in Rats.

Oxidative medicine and cellular longevity

Yang X,Niu N,Liang C,Wu MM,Tang LL,Wang QS,Lou J,Song BL,Zheng WW,Ma HP,Zhang ZR

44-1260G was used in Western Blotting to suggest that high salt intake stimulates endothelial cells to express and release bone morphogenetic protein 4 (BMP4) which reduces artery relaxation by stimulating the epithelial sodium channel in endothelial cells.

Thu May 20 00:00:00 EDT 2021

Stimulation of Epithelial Sodium Channels in Endothelial Cells by Bone Morphogenetic Protein-4 Contributes to Salt-Sensitive Hypertension in Rats.

Oxidative medicine and cellular longevity

Yang X,Niu N,Liang C,Wu MM,Tang LL,Wang QS,Lou J,Song BL,Zheng WW,Ma HP,Zhang ZR

Thu May 20 00:00:00 EDT 2021

Glucocorticoids and serum- and glucocorticoid-inducible kinase 1 are potent regulators of CFTR in the native intestine: implications for stress-induced diarrhea.

American journal of physiology. Gastrointestinal and liver physiology

Ahsan MK,Figueroa-Hall L,Baratta V,Garcia-Milian R,Lam TT,Hoque K,Salas PJ,Ameen NA

44-1260G was used in Western Blot to identify unique and druggable intestine-specific factors of the pathway that are targets for treating stress-induced diarrhea.

Sat Aug 01 00:00:00 EDT 2020

The effects of brain serotonin deficiency on behavioural disinhibition and anxiety-like behaviour following mild early life stress.

The international journal of neuropsychopharmacology

Sachs BD,Rodriguiz RM,Siesser WB,Kenan A,Royer EL,Jacobsen JP,Wetsel WC,Caron MG

44-1260G was used in western blot to determine the effects of early life maternal separation stress on anxiety-like behavior and behavioral disinhibition.

Tue Oct 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: serine/threonine protein kinase SGK; Serine/threonine-protein kinase Sgk1; serum and glucocorticoid-dependent protein kinase; Serum/glucocorticoid-regulated kinase 1; Sgk1 variant i3

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Gene Aliases: SGK; SGK1

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UniProt ID: (Human) O00141, (Mouse) Q9WVC6

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Entrez Gene ID: (Human) 6446, (Mouse) 20393, (Rat) 29517

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Function(s)

protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity calcium channel regulator activity protein binding ATP binding potassium channel regulator activity sodium channel regulator activity chloride channel regulator activity 3-phosphoinositide-dependent protein kinase binding cofactor binding tau protein binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups phosphatidylinositol binding protein modifying enzyme

Process(es)

regulation of cell growth protein phosphorylation sodium ion transport cellular sodium ion homeostasis apoptotic process response to stress cellular response to DNA damage stimulus microtubule depolymerization long-term memory regulation of blood pressure visual learning positive regulation of sodium ion transport peptidyl-serine phosphorylation positive regulation of cell growth regulation of cell migration negative regulation of microtubule polymerization positive regulation of transporter activity ion transmembrane transport intracellular signal transduction regulation of cell proliferation regulation of apoptotic process negative regulation of apoptotic process glucocorticoid mediated signaling pathway neuron projection morphogenesis positive regulation of dendrite morphogenesis regulation of catalytic activity regulation of sequence-specific DNA binding transcription factor activity regulation of cell cycle regulation of gastric acid secretion renal sodium ion absorption phosphorylation cellular response to insulin stimulus regulation of protein localization

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Phospho-SGK1 (Thr256) Polyclonal Antibody

Phospho-SGK1 (Thr256) Antibody (44-1260G) in IHC (P)

Product Details

44-1260G

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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