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Phospho-SHC3 (Tyr424) Antibody (PA5-106036) in ICC/IF
Immunocytochemistry analysis of Phospho-SHC3 (Tyr424) in Hela cells (serum starvation). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-SHC3 (Tyr424) polyclonal antibody (Product # PA5-106036) 1:200 and mouse anti-beta tubulin antibody (1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat ant... View More
Product Details
PA5-106036
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SHC3(Accession Q92529), corresponding to amino acid residues around phosphorylated Tyr424.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2817435
Product Specific Information
Antibody detects endogenous levels of Shc3 only when phosphorylated at Tyr424.
Target Information
Signaling adapter that couples activated growth factor receptors to signaling pathway in neurons. Involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: N-Shc; Neuronal Shc; Protein Rai; SH2 domain protein C3; SHC (Src homology 2 domain containing) transforming protein 3; SHC-transforming protein 3; SHC-transforming protein C; Shc3 p51; src homology 2 domain-containing transforming protein C3; Src homology 2 domain-containing-transforming protein C3
Gene Aliases: N-Shc; NSHC; RAI; SHC3; SHCC
UniProt ID: (Human) Q92529, (Mouse) Q3ZAX2
Entrez Gene ID: (Human) 53358, (Rat) 114858, (Mouse) 20418
MAPK cascade
transmembrane receptor protein tyrosine kinase signaling pathway
epidermal growth factor receptor signaling pathway
Ras protein signal transduction
central nervous system development
learning or memory
synaptic transmission, glutamatergic
positive regulation of GTPase activity
intracellular signal transduction
signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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