Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Phospho-SIK1/SIK2/SIK3 (Thr182, Thr175, Thr221) Polyclonal Antibody
FIGURE: 1 / 4
Phospho-SIK1/SIK2/SIK3 (Thr182, Thr175, Thr221) Antibody (PA5-105914) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-SIK2/SIK3 (Thr175, Thr163) in human lung cancer tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-SIK2/SIK3 (Thr175, Thr163) polyclonal antibody (Product # PA5-105914) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-ra... View More
Product Details
PA5-105914
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SIK1(Accession P57059), corresponding to amino acid residues around phosphorylated Thr182.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2817313
Product Specific Information
Specificity: Antibody detects endogenous levels of QIK/QSK only when phosphorylated at Thr182/Thr175/Thr221.
Target Information
Serine/threonine-protein kinase (SIK1) involved in various processes such as cell cycle regulation, gluconeogenesis and lipogenesis regulation, muscle growth and differentiation and tumor suppression. Phosphorylates HDAC4, HDAC5, PPME1, SREBF1, CRTC1/TORC1. Inhibits CREB activity by phosphorylating and inhibiting activity of TORCs, the CREB-specific coactivators, like CRTC2/TORC2 and CRTC3/TORC3 in response to cAMP signaling. Acts as a tumor suppressor and plays a key role in p53/TP53-dependent anoikis, a type of apoptosis triggered by cell detachment: required for phosphorylation of p53/TP53 in response to loss of adhesion and is able to suppress metastasis. Part of a sodium-sensing signaling network, probably by mediating phosphorylation of PPME1: following increases in intracellular sodium, SIK1 is activated by CaMK1 and phosphorylates PPME1 subunit of protein phosphatase 2A (PP2A), leading to dephosphorylation of sodium/potassium-transporting ATPase ATP1A1 and subsequent increase activity of ATP1A1. Acts as a regulator of muscle cells by phosphorylating and inhibiting class II histone deacetylases HDAC4 and HDAC5, leading to promote expression of MEF2 target genes in myocytes. Also required during cardiomyogenesis by regulating the exit of cardiomyoblasts from the cell cycle via down-regulation of CDKN1C/p57Kip2. Acts as a regulator of hepatic gluconeogenesis by phosphorylating and repressing the CREB-specific coactivators CRTC1/TORC1 and CRTC2/TORC2, leading to inhibit CREB activity. Also regulates hepatic lipogenesis by phosphorylating and inhibiting SREBF1. In concert with CRTC1/TORC1, regulates the light-induced entrainment of the circadian clock by attenuating PER1 induction; represses CREB-mediated transcription of PER1 by phosphorylating and deactivating CRTC1/TORC1. SIK2 phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators. SIK3 is a positive regulator of mTOR signaling that functions by triggering the degradation of DEPTOR, an mTOR inhibitor. Involved in the dynamic regulation of mTOR signaling in chondrocyte differentiation during skeletogenesis. Negatively regulates cAMP signaling pathway possibly by acting on CRTC2/TORC2 and CRTC3/TORC3 (Probable). Prevents HDAC4 translocation to the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: HRT-20; Myocardial SNF1-like kinase; Probable serine/threonine protein kinase SNF1LK (HRT-20) (Myocardial SNF1-like kinase); Protein kinase KID2; QIK; Qin-induced kinase; QSK; salt induceable kinase 2; Salt-inducible kinase 1; Salt-inducible kinase 2; Salt-inducible kinase 3; salt-inducible protein kinase 1; salt-inducible protein kinase 2; salt-inducible serine/threonine kinase 2; serine/threonine protein kinase; Serine/threonine-protein kinase QSK; Serine/threonine-protein kinase SIK1; Serine/threonine-protein kinase SIK2; Serine/threonine-protein kinase SIK3; Serine/threonine-protein kinase SNF1-like kinase 1; Serine/threonine-protein kinase SNF1-like kinase 2; Serine/threonine-protein kinase SNF1LK; SIK-1; SIK-2; SIK-3; SNF1-like kinase; SNF1-like kinase 2
Gene Aliases: 5730525O22Rik; 9030204A07Rik; AI447252; AI846254; AI849445; EIEE30; G630080D20Rik; KIAA0781; KIAA0999; Kid2; L19; LOH11CR1I; mKIAA0999; MSK; QIK; QSK; RGD1559698; SIK; SIK-2; SIK-3; SIK1; SIK2; SIK3; SNF1LK; SNF1LK2
UniProt ID: (Human) P57059, (Human) Q9H0K1, (Human) H0Y494, (Mouse) Q60670, (Rat) Q9R1U5, (Mouse) Q8CFH6, (Mouse) Q6P4S6
Entrez Gene ID: (Human) 150094, (Human) 23235, (Human) 23387, (Mouse) 17691, (Rat) 59329, (Mouse) 235344, (Rat) 315649, (Mouse) 70661, (Rat) 684112
magnesium ion binding
protein serine/threonine kinase activity
protein binding
ATP binding
cAMP response element binding protein binding
protein kinase binding
14-3-3 protein binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
histone deacetylase binding
metal ion binding
non-receptor serine/threonine protein kinase
regulation of sodium ion transport
protein phosphorylation
cell cycle
regulation of mitotic cell cycle
regulation of myotube differentiation
negative regulation of triglyceride biosynthetic process
negative regulation of CREB transcription factor activity
cellular response to hormone stimulus
intracellular signal transduction
entrainment of circadian clock by photoperiod
regulation of cell differentiation
negative regulation of gluconeogenesis
protein autophosphorylation
rhythmic process
cardiac muscle cell differentiation
positive regulation of anoikis
regulation of insulin receptor signaling pathway
negative regulation of transcription from RNA polymerase II promoter
multicellular organism development
phosphorylation
cell differentiation
anoikis
insulin receptor signaling pathway
endochondral ossification
regulation of protein localization
limb morphogenesis
multicellular organism growth
skeletal system morphogenesis
cartilage development involved in endochondral bone morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support