Invitrogen

Phospho-SMAD2 (Ser465, Ser467) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

23 Published Figures

39 References

View all (67) SMAD2 antibodies

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Protocols

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Protocols

Questions & Answers

Phospho-SMAD2 (Ser465, Ser467) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 33

Phospho-SMAD2 (Ser465, Ser467) Antibody (44-244G) in ICC/IF

Immunofluorescent analysis of Phospho-SMAD2 pSer465/pSer467 Antibody was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were treated with TGF-beta1 (20 ng/mL) for 15 minutes and labeled with Phospho-SMAD2 pSer465/pSer467 Antibody ... View More

Phospho-SMAD2 (Ser465, Ser467) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-SMAD2 (Ser465, Ser467) Antibody in Western Blot (WB)

Phospho-SMAD2 (Ser465, Ser467) Antibody in ChIP Assay (ChIP)

Phospho-SMAD2 (Ser465, Ser467) Antibody in Immunohistochemistry (IHC)

Phospho-SMAD2 (Ser465, Ser467) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

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Product Details

44-244G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 16 publications 16 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:250

View 5 publications 5 publications

ChIP assay (ChIP)

10 µL

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Smad2 that contains serines 465 and 467. The sequence is conserved in mouse and rat.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533614

Target Information

SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin. In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CVM-1118 (foslinanib), a 2-phenyl-4-quinolone derivative, promotes apoptosis and inhibits vasculogenic mimicry via targeting TRAP1.

Pathology oncology research : POR

Shen L,Chen YL,Huang CC,Shyu YC,Seftor REB,Seftor EA,Hendrix MJC,Chien DS,Chu YW

44-244G was used in Western Blotting to demonstrate CVM-1125 reduced the protein level of TRAP1 and impeded its downstream signaling by reduction of cellular succinate levels and destabilization of HIF-1α.

Mon Jun 26 00:00:00 EDT 2023

CVM-1118 (foslinanib), a 2-phenyl-4-quinolone derivative, promotes apoptosis and inhibits vasculogenic mimicry via targeting TRAP1.

Pathology oncology research : POR

Shen L,Chen YL,Huang CC,Shyu YC,Seftor REB,Seftor EA,Hendrix MJC,Chien DS,Chu YW

Mon Jun 26 00:00:00 EDT 2023

Protein phosphatase PPM1A inhibition attenuates osteoarthritis via regulating TGF-β/Smad2 signaling in chondrocytes.

JCI insight

Ge Q,Shi Z,Zou KA,Ying J,Chen J,Yuan W,Wang W,Xiao L,Lin X,Chen D,Feng XH,Wang PE,Tong P,Jin H

44-244G was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to identify PPM1A as a pivotal regulator of cartilage homeostasis and demonstrated that PPM1A inhibition attenuates OA progression via regulating TGF-β/SMAD2 signaling in chondrocytes and provided PPM1A as a potential target for OA treatment.

Wed Feb 08 00:00:00 EST 2023

The DACH1 gene is frequently deleted in prostate cancer, restrains prostatic intraepithelial neoplasia, decreases DNA damage repair, and predicts therapy responses.

Oncogene

Li Z,Jiao X,Robertson AG,Di Sante G,Ashton AW,DiRocco A,Wang M,Zhao J,Addya S,Wang C,McCue PA,South AP,Cordon-Cardo C,Liu R,Patel K,Hamid R,Parmar J,DuHadaway JB,Jones SJM,Casimiro MC,Schultz N,Kossenkov A,Phoon LY,Chen H,Lan L,Sun Y,Iczkowski KA,Rui H,Pestell RG

44-244G was used in Immunohistochemistry to investigate the role of the DACH1 gene in prostate cancer (PCa), revealing that its deletion is associated with increased androgen receptor (AR) activity, poor prognosis, enhanced prostate neoplasia in mice, increased DNA damage response, augmented DNA repair mechanisms, and resistance to PARP inhibitors and TGFβ kinase inhibitors.

Thu Jun 01 00:00:00 EDT 2023

Targeting TGF-β for treatment of osteogenesis imperfecta.

The Journal of clinical investigation

Song IW,Nagamani SC,Nguyen D,Grafe I,Sutton VR,Gannon FH,Munivez E,Jiang MM,Tran A,Wallace M,Esposito P,Musaad S,Strudthoff E,McGuire S,Thornton M,Shenava V,Rosenfeld S,Huang S,Shypailo R,Orwoll E,Lee B

Published figure using Phospho-SMAD2 (Ser465, Ser467) polyclonal antibody (Product # 44-244G) in Immunohistochemistry

Fri Apr 01 00:00:00 EDT 2022

Targeting TGF-β for treatment of osteogenesis imperfecta.

The Journal of clinical investigation

Published figure using Phospho-SMAD2 (Ser465, Ser467) polyclonal antibody (Product # 44-244G) in Immunohistochemistry

Fri Apr 01 00:00:00 EDT 2022

Periosteal CD68⁺ F4/80⁺ Macrophages Are Mechanosensitive for Cortical Bone Formation by Secretion and Activation of TGF-β1.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Deng R,Li C,Wang X,Chang L,Ni S,Zhang W,Xue P,Pan D,Wan M,Deng L,Cao X

44-244G was used in Immunohistochemistry-frozen immunofluorescence to suggest that periosteal myeloid-lineage cells respond to mechanical forces and consequently produce and activate TGF-β1 for periosteal bone formation.

Sat Jan 01 00:00:00 EST 2022

Periosteal CD68⁺ F4/80⁺ Macrophages Are Mechanosensitive for Cortical Bone Formation by Secretion and Activation of TGF-β1.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Deng R,Li C,Wang X,Chang L,Ni S,Zhang W,Xue P,Pan D,Wan M,Deng L,Cao X

Sat Jan 01 00:00:00 EST 2022

Fucoidan-Mediated Inhibition of Fibrotic Properties in Oral Submucous Fibrosis via the MEG3/miR-181a/Egr1 Axis.

Pharmaceuticals (Basel, Switzerland)

Fang CY,Chen SH,Huang CC,Liao YW,Chao SC,Yu CC

44-244G was used in Western Blotting to investigate the anti-fibrotic effect of fucoidan on oral fibrosis.

Tue Jul 05 00:00:00 EDT 2022

TGF-β1 increases permeability of ciliated airway epithelia via redistribution of claudin 3 from tight junction into cell nuclei.

Pflugers Archiv : European journal of physiology

Schilpp C,Lochbaum R,Braubach P,Jonigk D,Frick M,Dietl P,Wittekindt OH

44-244G was used in Immunocytochemistry to conclude that the observed effects contribute to loss of epithelial integrity during atopic asthma.

Mon Feb 01 00:00:00 EST 2021

Co-inhibition of SMAD and MAPK signaling enhances 124I uptake in BRAF-mutant thyroid cancers.

Endocrine-related cancer

Luckett KA,Cracchiolo JR,Krishnamoorthy GP,Leandro-Garcia LJ,Nagarajah J,Saqcena M,Lester R,Im SY,Zhao Z,Lowe SW,de Stanchina E,Sherman EJ,Ho AL,Leach SD,Knauf JA,Fagin JA

44-244G was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to investigate the role of TGFB1 on thyroid-differentiated gene expression and RAI uptake in vivo.

Tue May 18 00:00:00 EDT 2021

Cholesterol Pathway Inhibition Induces TGF-β Signaling to Promote Basal Differentiation in Pancreatic Cancer.

Cancer cell

Gabitova-Cornell L,Surumbayeva A,Peri S,Franco-Barraza J,Restifo D,Weitz N,Ogier C,Goldman AR,Hartman TR,Francescone R,Tan Y,Nicolas E,Shah N,Handorf EA,Cai KQ,O'Reilly AM,Sloma I,Chiaverelli R,Moffitt RA,Khazak V,Fang CY,Golemis EA,Cukierman E,Astsaturov I

44-244G was used in Western Blot, Immunohistochemistry to dissect the causal relationship between endogenous cholesterol metabolism and pancreatic ductal adenocarcinoma development and differentiation.

Mon Oct 12 00:00:00 EDT 2020

Cholesterol Pathway Inhibition Induces TGF-β Signaling to Promote Basal Differentiation in Pancreatic Cancer.

Cancer cell

Mon Oct 12 00:00:00 EDT 2020

The Immunosuppressive Microenvironment in BRCA1-IRIS-Overexpressing TNBC Tumors Is Induced by Bidirectional Interaction with Tumor-Associated Macrophages.

Cancer research

Sami E,Paul BT,Koziol JA,ElShamy WM

44-244G was used in Western Blot to investigate the effects of BRCA1-IRIS over-expression on macrophage recruitment and the production of protumour M2 TAMs.

Sun Mar 01 00:00:00 EST 2020

Aggressive Mammary Cancers Lacking Lymphocytic Infiltration Arise in Irradiated Mice and Can Be Prevented by Dietary Intervention.

Cancer immunology research

Omene C,Ma L,Moore J,Ouyang H,Illa-Bochaca I,Chou W,Patel MS,Sebastiano C,Demaria S,Mao JH,Karagoz K,Gatza ML,Barcellos-Hoff MH

Published figure using Phospho-SMAD2 (Ser465, Ser467) polyclonal antibody (Product # 44-244G) in Immunohistochemistry

Sat Feb 01 00:00:00 EST 2020

Prostate epithelial-specific expression of activated PI3K drives stromal collagen production and accumulation.

The Journal of pathology

Wegner KA,Mueller BR,Unterberger CJ,Avila EJ,Ruetten H,Turco AE,Oakes SR,Girardi NM,Halberg RB,Swanson SM,Marker PC,Vezina CM

44-244G was used in Immunohistochemistry to indicate a signaling process beginning with prostatic epithelial PI3K and TGF-β signaling that drives prostatic stromal hypertrophy and collagen accumulation.

Sat Feb 01 00:00:00 EST 2020

Prostate epithelial-specific expression of activated PI3K drives stromal collagen production and accumulation.

The Journal of pathology

Wegner KA,Mueller BR,Unterberger CJ,Avila EJ,Ruetten H,Turco AE,Oakes SR,Girardi NM,Halberg RB,Swanson SM,Marker PC,Vezina CM

Sat Feb 01 00:00:00 EST 2020

Differential Role of Smad2 and Smad3 in the Acquisition of an Endovascular Trophoblast-Like Phenotype and Preeclampsia.

Frontiers in endocrinology

Brkić J,Dunk C,Shan Y,O'Brien JA,Lye P,Qayyum S,Yang P,Matthews SG,Lye SJ,Peng C

44-244G was used in Immunohistochemistry to suggest that Smad2 and Smad3 have opposite functions in the acquisition of an enEVT-like phenotype and defects in Smad3 activation are associated with PE.

Fri May 28 00:00:00 EDT 2021

Differential Role of Smad2 and Smad3 in the Acquisition of an Endovascular Trophoblast-Like Phenotype and Preeclampsia.

Frontiers in endocrinology

Brkić J,Dunk C,Shan Y,O'Brien JA,Lye P,Qayyum S,Yang P,Matthews SG,Lye SJ,Peng C

44-244G was used in Immunohistochemistry to suggest that Smad2 and Smad3 have opposite functions in the acquisition of an enEVT-like phenotype and defects in Smad3 activation are associated with PE.

Fri May 28 00:00:00 EDT 2021

Differential Role of Smad2 and Smad3 in the Acquisition of an Endovascular Trophoblast-Like Phenotype and Preeclampsia.

Frontiers in endocrinology

Brkić J,Dunk C,Shan Y,O'Brien JA,Lye P,Qayyum S,Yang P,Matthews SG,Lye SJ,Peng C

44-244G was used in Immunohistochemistry to suggest that Smad2 and Smad3 have opposite functions in the acquisition of an enEVT-like phenotype and defects in Smad3 activation are associated with PE.

Fri May 28 00:00:00 EDT 2021

Obesity reduces mammary epithelial cell TGFβ1 activity through macrophage-mediated extracellular matrix remodeling.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Chamberlin T,Thompson V,Hillers-Ziemer LE,Walton BN,Arendt LM

44-244G was used in Immunohistochemistry-immunofluorescence to show TGFβ1 activity is reduced in the breast epithelial cells in obesity.

Mon Jun 01 00:00:00 EDT 2020

Role of sialidase Neu3 and ganglioside GM3 in cardiac fibroblasts activation.

The Biochemical journal

Ghiroldi A,Piccoli M,Creo P,Cirillo F,Rota P,D'Imperio S,Ciconte G,Monasky MM,Micaglio E,Garatti A,Aureli M,Carsana EV,Menicanti L,Pappone C,Anastasia L

44-244G was used in Western Blot to identify pro-fibrotic pathways in primary cultures of human cardiac fibroblasts as targets for cardiac fibrosis.

Fri Sep 18 00:00:00 EDT 2020

Sauchinone Protects Renal Mesangial Cell Dysfunction against Angiotensin II by Improving Renal Fibrosis and Inflammation.

International journal of molecular sciences

Yoon JJ,Lee HK,Kim HY,Han BH,Lee HS,Lee YJ,Kang DG

44-244G was used in Immunocytochemistry to investigate the role of sauchinone in mesangial cell proliferation and fibrosis induced by angiotensin II, as well as the underlying mechanisms of these processes.

Wed Sep 23 00:00:00 EDT 2020

Sauchinone Protects Renal Mesangial Cell Dysfunction against Angiotensin II by Improving Renal Fibrosis and Inflammation.

International journal of molecular sciences

Yoon JJ,Lee HK,Kim HY,Han BH,Lee HS,Lee YJ,Kang DG

Wed Sep 23 00:00:00 EDT 2020

Sauchinone Protects Renal Mesangial Cell Dysfunction against Angiotensin II by Improving Renal Fibrosis and Inflammation.

International journal of molecular sciences

Yoon JJ,Lee HK,Kim HY,Han BH,Lee HS,Lee YJ,Kang DG

Wed Sep 23 00:00:00 EDT 2020

A Novel, Pan-PDE Inhibitor Exerts Anti-Fibrotic Effects in Human Lung Fibroblasts via Inhibition of TGF-β Signaling and Activation of cAMP/PKA Signaling.

International journal of molecular sciences

Wójcik-Pszczoła K,Chłoń-Rzepa G,Jankowska A,Ślusarczyk M,Ferdek PE,Kusiak AA,Świerczek A,Pociecha K,Koczurkiewicz-Adamczyk P,Wyska E,Pękala E,Gosens R

44-244G was used in Immunocytochemistry-immunoflourescence to investigate the effect of selected derivatives (832-a pan-PDE inhibitor, 869-a TRPA1 modulator, and 145-a pan-PDE inhibitor and a weak TRPA1 modulator) on cellular responses related to airway remodeling using MRC-5 human lung fibroblasts.

Wed Jun 03 00:00:00 EDT 2020

A Novel, Pan-PDE Inhibitor Exerts Anti-Fibrotic Effects in Human Lung Fibroblasts via Inhibition of TGF-β Signaling and Activation of cAMP/PKA Signaling.

International journal of molecular sciences

Wójcik-Pszczoła K,Chłoń-Rzepa G,Jankowska A,Ślusarczyk M,Ferdek PE,Kusiak AA,Świerczek A,Pociecha K,Koczurkiewicz-Adamczyk P,Wyska E,Pękala E,Gosens R

Wed Jun 03 00:00:00 EDT 2020

A Novel, Pan-PDE Inhibitor Exerts Anti-Fibrotic Effects in Human Lung Fibroblasts via Inhibition of TGF-β Signaling and Activation of cAMP/PKA Signaling.

International journal of molecular sciences

Wójcik-Pszczoła K,Chłoń-Rzepa G,Jankowska A,Ślusarczyk M,Ferdek PE,Kusiak AA,Świerczek A,Pociecha K,Koczurkiewicz-Adamczyk P,Wyska E,Pękala E,Gosens R

Wed Jun 03 00:00:00 EDT 2020

The neovascularization effect of dedifferentiated fat cells.

Scientific reports

Watanabe H,Goto S,Kato R,Komiyama S,Nagaoka Y,Kazama T,Yamamoto C,Li Y,Konuma N,Hagikura K,Matsumoto T

Published figure using Phospho-SMAD2 (Ser465, Ser467) polyclonal antibody (Product # 44-244G) in Western Blot

Mon Jun 08 00:00:00 EDT 2020

The neovascularization effect of dedifferentiated fat cells.

Scientific reports

Watanabe H,Goto S,Kato R,Komiyama S,Nagaoka Y,Kazama T,Yamamoto C,Li Y,Konuma N,Hagikura K,Matsumoto T

Published figure using Phospho-SMAD2 (Ser465, Ser467) polyclonal antibody (Product # 44-244G) in Western Blot

Mon Jun 08 00:00:00 EDT 2020

The motor protein Myo1c regulates transforming growth factor-β-signaling and fibrosis in podocytes.

Kidney international

Arif E,Solanki AK,Srivastava P,Rahman B,Tash BR,Holzman LB,Janech MG,Martin R,Knölker HJ,Fitzgibbon WR,Deng P,Budisavljevic MN,Syn WK,Wang C,Lipschutz JH,Kwon SH,Nihalani D

44-244G was used in Western Blotting to study the role of myosin(Myo)1c for transforming growth factor-β(TGF-β)-signaling in podocyte disease pathogenesis.

Mon Jul 01 00:00:00 EDT 2019

Therapeutic discovery for marrow failure with MDS predisposition using pluripotent stem cells.

JCI insight

Ruiz-Gutierrez M,Bölükbaşı ÖV,Alexe G,Kotini AG,Ballotti K,Joyce CE,Russell DW,Stegmaier K,Myers K,Novina CD,Papapetrou EP,Shimamura A

Published figure using Phospho-SMAD2 (Ser465, Ser467) polyclonal antibody (Product # 44-244G) in Immunofluorescence

Tue Apr 30 00:00:00 EDT 2019

Pancreatic Ductal Deletion of Hnf1b Disrupts Exocrine Homeostasis, Leads to Pancreatitis, and Facilitates Tumorigenesis.

Cellular and molecular gastroenterology and hepatology

Quilichini E,Fabre M,Dirami T,Stedman A,De Vas M,Ozguc O,Pasek RC,Cereghini S,Morillon L,Guerra C,Couvelard A,Gannon M,Haumaitre C

Published figure using Phospho-SMAD2 (Ser465, Ser467) polyclonal antibody (Product # 44-244G) in Immunohistochemistry

Mon Jul 20 00:00:00 EDT 2020

Cancer-associated stroma significantly contributes to the mesenchymal subtype signature of serous ovarian cancer.

Gynecologic oncology

Zhang Q,Wang C,Cliby WA

44-244G was used in Immunohistochemistry to determine the origins of multiple mesenchymal subtype gene signatures in high-grade serous ovarian cancer.

Fri Feb 01 00:00:00 EST 2019

Loss of EMILIN-1 Enhances Arteriolar Myogenic Tone Through TGF-β (Transforming Growth Factor-β)-Dependent Transactivation of EGFR (Epidermal Growth Factor Receptor) and Is Relevant for Hypertension in Mice and Humans.

Arteriosclerosis, thrombosis, and vascular biology

Carnevale D,Facchinello N,Iodice D,Bizzotto D,Perrotta M,De Stefani D,Pallante F,Carnevale L,Ricciardi F,Cifelli G,Da Ros F,Casaburo M,Fardella S,Bonaldo P,Innocenzi G,Rizzuto R,Braghetta P,Lembo G,Bressan GM

44-244G was used in Immunohistochemistry-immunofluorescence to implicate an unexpected role of the TGF-β-EGFR pathway in hypertension with current translational perspectives.

Mon Oct 01 00:00:00 EDT 2018

Snail regulates BMP and TGFβ pathways to control the differentiation status of glioma-initiating cells.

Oncogene

Caja L,Tzavlaki K,Dadras MS,Tan EJ,Hatem G,Maturi NP,Morén A,Wik L,Watanabe Y,Savary K,Kamali-Moghaddan M,Uhrbom L,Heldin CH,Moustakas A

Published figure using Phospho-SMAD2 (Ser465, Ser467) polyclonal antibody (Product # 44-244G) in Immunohistochemistry

Tue May 01 00:00:00 EDT 2018

Oncogenic Kras drives invasion and maintains metastases in colorectal cancer.

Genes & development

Boutin AT,Liao WT,Wang M,Hwang SS,Karpinets TV,Cheung H,Chu GC,Jiang S,Hu J,Chang K,Vilar E,Song X,Zhang J,Kopetz S,Futreal A,Wang YA,Kwong LN,DePinho RA

Published figure using Phospho-SMAD2 (Ser465, Ser467) polyclonal antibody (Product # 44-244G) in Immunohistochemistry

Wed Feb 15 00:00:00 EST 2017

Myostatin propeptide mutation of the hypermuscular Compact mice decreases the formation of myostatin and improves insulin sensitivity.

American journal of physiology. Endocrinology and metabolism

Kocsis T,Trencsenyi G,Szabo K,Baan JA,Muller G,Mendler L,Garai I,Reinauer H,Deak F,Dux L,Keller-Pinter A

44244G was used in western blot to determine the effect of the Mstn(Cmpt-dl1Abc) mutation or the Compact genetic background on morphology, metabolism and signaling

Wed Mar 01 00:00:00 EST 2017

A novel bifunctional anti-PD-L1/TGF-β Trap fusion protein (M7824) efficiently reverts mesenchymalization of human lung cancer cells.

Oncoimmunology

David JM,Dominguez C,McCampbell KK,Gulley JL,Schlom J,Palena C

44-244G was used in Immunohistochemistry to investigate the association between TGF-Β1-mediated mesenchymalisation and PD-L1 expression in non-small cell lung cancer cells.

Tue Jan 12 00:00:00 EST 2021

Conditional ablation of TGF-β signaling inhibits tumor progression and invasion in an induced mouse bladder cancer model.

Scientific reports

Liang Y,Zhu F,Zhang H,Chen D,Zhang X,Gao Q,Li Y

44-244G was used in immunohistochemistry - paraffin section investigate the role of TGF-beta signaling in bladder cancer progression

Tue Jul 05 00:00:00 EDT 2016

Melanocytes Affect Nodal Expression and Signaling in Melanoma Cells: A Lesson from Pediatric Large Congenital Melanocytic Nevi.

International journal of molecular sciences

Margaryan NV,Gilgur A,Seftor EA,Purnell C,Arva NC,Gosain AK,Hendrix MJ,Strizzi L

44-244G was used in western blot to research pediatric large congenital melanocytic nevi to show melanocytes affect nodal expression and signaling in melanoma cells

Tue Mar 22 00:00:00 EDT 2016

Effects of a novel Nodal-targeting monoclonal antibody in melanoma.

Oncotarget

Strizzi L,Sandomenico A,Margaryan NV,Focà A,Sanguigno L,Bodenstine TM,Chandler GS,Reed DW,Gilgur A,Seftor EA,Seftor RE,Khalkhali-Ellis Z,Leonardi A,Ruvo M,Hendrix MJ

44-244G was used in western blot to assess the use of 3D1 mAb for treating Nodal expressing cancers.

Tue Oct 27 00:00:00 EDT 2015

Effects of a novel Nodal-targeting monoclonal antibody in melanoma.

Oncotarget

Strizzi L,Sandomenico A,Margaryan NV,Focà A,Sanguigno L,Bodenstine TM,Chandler GS,Reed DW,Gilgur A,Seftor EA,Seftor RE,Khalkhali-Ellis Z,Leonardi A,Ruvo M,Hendrix MJ

44-244G was used in western blot to assess the use of 3D1 mAb for treating Nodal expressing cancers.

Tue Oct 27 00:00:00 EDT 2015

BMP signaling controls muscle mass.

Nature genetics

Sartori R,Schirwis E,Blaauw B,Bortolanza S,Zhao J,Enzo E,Stantzou A,Mouisel E,Toniolo L,Ferry A,Stricker S,Goldberg AL,Dupont S,Piccolo S,Amthor H,Sandri M

44-244G was used in western blot to demonstrate that bone morphogenetic protein signaling, acting through Smad, Smad5 and Smad8, is the fundamental hypertrophic signal in mice.

Fri Nov 01 00:00:00 EDT 2013

The significance of a Cripto-1 positive subpopulation of human melanoma cells exhibiting stem cell-like characteristics.

Cell cycle (Georgetown, Tex.)

Strizzi L,Margaryan NV,Gilgur A,Hardy KM,Normanno N,Salomon DS,Hendrix MJ

44-244G was used in western blot to investigate the role of cell surface CR- expression in human melanoma cells.

Wed May 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: hMAD-2; JV18-1; MAD; MAD homolog 2; Mad protein homolog; Mad-related protein 2; MGC22139; MGC34440; mMad2; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; mothers against DPP homolog 2; pSMAD2; Sma- and Mad-related protein 2; SMAD; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2; Smad2Deltaexon3

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Gene Aliases: 7120426M23Rik; hMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2; mMad2; Smad-2; SMAD2

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UniProt ID: (Human) Q15796, (Mouse) Q62432

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Entrez Gene ID: (Human) 4087, (Mouse) 17126

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding chromatin binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding transforming growth factor beta receptor binding protein binding transcription factor binding phosphatase binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity ubiquitin protein ligase binding activating transcription factor binding type I transforming growth factor beta receptor binding enhancer binding protein homodimerization activity SMAD binding metal ion binding protein heterodimerization activity co-SMAD binding I-SMAD binding R-SMAD binding DNA-binding transcription factor gene-specific transcriptional regulator

Process(es)

negative regulation of transcription from RNA polymerase II promoter ureteric bud development in utero embryonic development endoderm formation mesoderm formation transcription from RNA polymerase II promoter transforming growth factor beta receptor signaling pathway common-partner SMAD protein phosphorylation SMAD protein complex assembly zygotic specification of dorsal/ventral axis gastrulation negative regulation of cell proliferation response to glucose post-embryonic development anterior/posterior pattern specification positive regulation of epithelial to mesenchymal transition regulation of transforming growth factor beta receptor signaling pathway signal transduction involved in regulation of gene expression insulin secretion lung development negative regulation of transforming growth factor beta receptor signaling pathway positive regulation of BMP signaling pathway pancreas development primary miRNA processing activin receptor signaling pathway somatic stem cell population maintenance organ growth intracellular signal transduction nodal signaling pathway cell fate commitment negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter paraxial mesoderm morphogenesis embryonic foregut morphogenesis embryonic cranial skeleton morphogenesis regulation of binding palate development pericardium development SMAD protein signal transduction response to cholesterol positive regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation pattern specification process endoderm development heart development embryonic pattern specification positive regulation of gene expression negative regulation of gene expression developmental growth

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Phospho-SMAD2 (Ser465, Ser467) Polyclonal Antibody

Phospho-SMAD2 (Ser465, Ser467) Antibody (44-244G) in ICC/IF

Product Details

44-244G

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

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