Invitrogen

Phospho-SMAD3 (Ser423, Ser425) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 Published Figures

16 References

View all (62) SMAD3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Phospho-SMAD3 (Ser423, Ser425) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-SMAD3 (Ser423, Ser425) Antibody (44-246G) in ICC/IF

Immunofluorescence analysis of SMAD3 (pSpS423/425) was done on 70% confluent log phase HeLa cells treated with TGF-beta (20 ng/mL for 15 min) cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with SMAD3 (pSpS423/425) Rabbit polyclonal Antibody (Product # 44... View More

Phospho-SMAD3 (Ser423, Ser425) Antibody in Western Blot (WB)

Phospho-SMAD3 (Ser423, Ser425) Antibody in Immunohistochemistry (IHC)

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Product Details

44-246G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:250

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Hamster, Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Smad3 that contains serine 423 and serine 425. This sequence is conserved in mouse.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533615

Target Information

SMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MircroRNA-92b as a negative regulator of the TGF-β signaling by targeting the type I receptor.

iScience

Yang S,Jiang K,Li L,Xiang J,Li Y,Kang L,Yang G,Liang Z

Published figure using Phospho-SMAD3 (Ser423, Ser425) polyclonal antibody (Product # 44-246G) in Western Blot

Fri Nov 17 00:00:00 EST 2023

Hyperglycaemia-induced impairment of the autorhythmicity and gap junction activity of mouse embryonic stem cell-derived cardiomyocyte-like cells.

Histochemistry and cell biology

Menzele A,Aboalgasm H,Ballo R,Gwanyanya A

44-246G was used in Western Blotting to investigate the effect of hyperglycaemia on the pulsatile activity of stem cell-derived cardiomyocytes.

Sat Apr 01 00:00:00 EDT 2023

SMOC2 promoted vascular smooth muscle cell proliferation, migration, and extracellular matrix degradation by activating BMP/TGF-β1 signaling pathway.

Journal of clinical biochemistry and nutrition

Wang X,Wang M,Zhou Z,Zou X,Song G,Zhang Q,Zhou H

44-246G was used in Western Blotting to evaluate the function of SMOC2 in abdominal aortic aneurysm.

Fri Sep 01 00:00:00 EDT 2023

SMAD3 contributes to ascending aortic dilatation independent of transforming growth factor-beta in bicuspid and unicuspid aortic valve disease.

Scientific reports

Balint B,Federspiel J,Kollmann C,Teping P,Schwab T,Schäfers HJ

44-246G was used in Immunohistochemistry (Paraffin) to determine whether there are differences in transforming growth factor-beta (TGFß) signaling in aneurysms associated with bicuspid (BAV) and unicuspid (UAV) aortic valves versus normal aortic valves.

Wed Sep 14 00:00:00 EDT 2022

SMAD3 contributes to ascending aortic dilatation independent of transforming growth factor-beta in bicuspid and unicuspid aortic valve disease.

Scientific reports

Balint B,Federspiel J,Kollmann C,Teping P,Schwab T,Schäfers HJ

Wed Sep 14 00:00:00 EDT 2022

Echinochrome A Treatment Alleviates Fibrosis and Inflammation in Bleomycin-Induced Scleroderma.

Marine drugs

Park GT,Yoon JW,Yoo SB,Song YC,Song P,Kim HK,Han J,Bae SJ,Ha KT,Mishchenko NP,Fedoreyev SA,Stonik VA,Kim MB,Kim JH

Published figure using Phospho-SMAD3 (Ser423, Ser425) polyclonal antibody (Product # 44-246G) in Immunohistochemistry

Fri Apr 23 00:00:00 EDT 2021

Sauchinone Protects Renal Mesangial Cell Dysfunction against Angiotensin II by Improving Renal Fibrosis and Inflammation.

International journal of molecular sciences

Yoon JJ,Lee HK,Kim HY,Han BH,Lee HS,Lee YJ,Kang DG

44-246G was used in Immunocytochemistry to investigate the role of sauchinone in mesangial cell proliferation and fibrosis induced by angiotensin II, as well as the underlying mechanisms of these processes.

Wed Sep 23 00:00:00 EDT 2020

Inhibition of TGF-β1 Signaling by IL-15: A Novel Role for IL-15 in the Control of Renal Epithelial-Mesenchymal Transition: IL-15 Counteracts TGF-β1-Induced EMT in Renal Fibrosis.

International journal of cell biology

Devocelle A,Lecru L,François H,Desterke C,Gallerne C,Eid P,Estelle O,Azzarone B,Giron-Michel J

44-246G was used in Western Blot to analyse whether human chronic inflammatory nephropathies evolving towards fibrosis could be also characterized by a weak intrarenal IL-15 expression and if IL-15 could inhibit epithelial-mesenchymal transition (EMT) and excess matrix deposition in human renal proximal tubular epithelial cells (RPTEC).

Wed Sep 30 00:00:00 EDT 2020

Formyl Peptide Receptor 2 Activation Ameliorates Dermal Fibrosis and Inflammation in Bleomycin-Induced Scleroderma.

Frontiers in immunology

Park GT,Kwon YW,Lee TW,Kwon SG,Ko HC,Kim MB,Kim JH

44-246G was used in Immunohistochemistry to investigate the effects of Fpr2 activation in the treatment of scleroderma fibrosis.

Mon Nov 09 00:00:00 EST 2020

Betulinic acid prevents high glucose‑induced expression of extracellular matrix protein in cardiac fibroblasts by inhibiting the TGF‑β1/Smad signaling pathway.

Molecular medicine reports

Jiang L,Chen FX,Zang ST,Yang QF

44-246G was used in Western Blotting to study the effect of betulinic acid on high glucose induced cardiac fibroblasts and study the mechanism underlying this effect.

Wed Nov 01 00:00:00 EDT 2017

Restenosis Inhibition and Re-differentiation of TGFβ/Smad3-activated Smooth Muscle Cells by Resveratrol.

Scientific reports

Zhu Y,Takayama T,Wang B,Kent A,Zhang M,Binder BY,Urabe G,Shi Y,DiRenzo D,Goel SA,Zhou Y,Little C,Roenneburg DA,Shi XD,Li L,Murphy WL,Kent KC,Ke J,Guo LW

44246G was used in western blot to study the effects of periadventitial delivery of resveratrol on three major pro-restenotic pathologies

Mon Feb 06 00:00:00 EST 2017

Genetic deletion of cell division autoantigen 1 retards diabetes-associated renal injury.

Journal of the American Society of Nephrology : JASN

Chai Z,Dai A,Tu Y,Li J,Wu T,Wang Y,Hale LJ,Koentgen F,Thomas MC,Cooper ME

44-246G was used in immunohistochemistry to study the role of cell division autoantigen 1 in diabetes-associated renal injury

Fri Nov 01 00:00:00 EDT 2013

Impaired angiogenic response in the corneas of mice lacking osteopontin.

Investigative ophthalmology & visual science

Fujita N,Fujita S,Okada Y,Fujita K,Kitano A,Yamanaka O,Miyamoto T,Kon S,Uede T,Rittling SR,Denhardt DT,Saika S

Mon Feb 01 00:00:00 EST 2010

Localization of phosphorylated SMAD proteins in granulosa cells, oocytes and oviduct of female mice.

Gene expression patterns : GEP

Tian X,Halfhill AN,Diaz FJ

Mon Jul 05 00:00:00 EDT 2010

BRCA1 interacts with Smad3 and regulates Smad3-mediated TGF-beta signaling during oxidative stress responses.

PloS one

Li H,Sekine M,Seng S,Avraham S,Avraham HK

Mon Sep 21 00:00:00 EDT 2009

BRCA1 interacts with Smad3 and regulates Smad3-mediated TGF-beta signaling during oxidative stress responses.

PloS one

Li H,Sekine M,Seng S,Avraham S,Avraham HK

Mon Sep 21 00:00:00 EDT 2009

Activin inhibits the human Pit-1 gene promoter through the p38 kinase pathway in a Smad-independent manner.

Endocrinology

de Guise C,Lacerte A,Rafiei S,Reynaud R,Roy M,Brue T,Lebrun JJ

Fri Sep 01 00:00:00 EDT 2006

Activin inhibits the human Pit-1 gene promoter through the p38 kinase pathway in a Smad-independent manner.

Endocrinology

de Guise C,Lacerte A,Rafiei S,Reynaud R,Roy M,Brue T,Lebrun JJ

Fri Sep 01 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: DKFZp586N0721; DKFZp686J10186; hMAD-3; hSMAD3; JV15-2; MAD homolog 3; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; MAD-3; mad3; MGC60396; mMad3; Mothers against decapentaplegic homolog 3; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD; SMAD 3; SMAD family member 3; SMAD, mothers against DPP homolog 3

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Gene Aliases: AU022421; HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3; SMAD3

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UniProt ID: (Human) P84022, (Mouse) Q8BUN5

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Entrez Gene ID: (Human) 4088, (Mouse) 17127

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter sequence-specific core promoter proximal region sequence-specific DNA binding transcription factor activity, protein binding RNA polymerase II activating transcription factor binding transcription factor activity, sequence-specific DNA binding transforming growth factor beta receptor binding protein binding collagen binding transcription factor binding zinc ion binding protein kinase binding phosphatase binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity chromatin DNA binding ubiquitin protein ligase binding enhancer binding identical protein binding protein homodimerization activity ubiquitin binding bHLH transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding protein heterodimerization activity co-SMAD binding R-SMAD binding DNA binding chromatin binding double-stranded DNA binding beta-catenin binding enzyme binding SMAD binding metal ion binding DNA-binding transcription factor gene-specific transcriptional regulator

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-SMAD3 (Ser423, Ser425) Polyclonal Antibody

Phospho-SMAD3 (Ser423, Ser425) Antibody (44-246G) in ICC/IF

Product Details

44-246G

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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