Invitrogen

Phospho-STAT5 (Tyr694) Monoclonal Antibody (SRBCZX), FITC, eBioscience™

7 Published Figures
10 References
View all (104) STAT5 alpha antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

11-9010-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (1 µg)/test
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Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

SRBCZX

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572520

Product Specific Information

Description: This SRBCZX monoclonal antibody recognizes signal transducer and activator of transcription 5 (STAT5) when phosphorylated on tyrosine 694. STAT proteins are activated by ligand binding to receptors, such as cytokine receptors, that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. In response to cytokines that signal through the common gamma chain such as IL-2, IL-7, and IL-15, STAT5 is phosphorylated on tyrosine 694 by JAK1 and JAK3. Cytokines such as IL-3, IL-5, and GM-CSF that signal via the common beta chain induce STAT5 phosphorylation on tyrosine 694 by JAK 2. Phosphorylation of STAT5 on tyrosine 694 is essential for STAT5 dimer formation, nuclear translocation, and DNA binding activity.

Specificity of this SRBCZX clone was determined by ELISA and flow cytometry.

Applications Reported: This SRBCZX antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This SRBCZX antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated U937 cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol. Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins and Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used. All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5 alpha is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of STAT5 alpha is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells. STAT5 alpha, along with STAT5 beta, were identified in mouse. The amino acid sequences of STAT5 alpha and STAT5 beta show 96% sequence similarity, and both proteins are co expressed in most tissues in virgin and lactating mice. However, differential accumulation of STAT5 alpha and STAT5 beta mRNA has been reported for both muscle and mammary tissue. STAT5 alpha is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Mammary gland factor; mammary gland factor STAT5A; Signal transducer and activator of transcription 5A; STAT; STAT5B

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Gene Aliases: AA959963; MGF; Mpf; STAT5; STAT5A

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UniProt ID: (Human) P42229, (Mouse) P42230

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Entrez Gene ID: (Human) 6776, (Mouse) 20850

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Function(s)
DNA binding transcription factor activity, sequence-specific DNA binding protein tyrosine kinase activity signal transducer activity protein binding RNA polymerase II core promoter sequence-specific DNA binding double-stranded DNA binding protein phosphatase binding sequence-specific DNA binding DNA-binding transcription factor
Process(es)
allantoin metabolic process positive regulation of endothelial cell proliferation citrate metabolic process 2-oxoglutarate metabolic process succinate metabolic process oxaloacetate metabolic process transcription, DNA-templated regulation of transcription from RNA polymerase II promoter isoleucine metabolic process valine metabolic process creatine metabolic process fatty acid metabolic process JAK-STAT cascade lactation peptidyl-tyrosine phosphorylation taurine metabolic process prolactin signaling pathway regulation of multicellular organism growth positive regulation of blood vessel endothelial cell migration creatinine metabolic process JAK-STAT cascade involved in growth hormone signaling pathway luteinization natural killer cell differentiation regulation of transcription, DNA-templated transcription from RNA polymerase II promoter signal transduction female pregnancy positive regulation of cell proliferation regulation of steroid metabolic process cytokine-mediated signaling pathway lipid storage regulation of cell adhesion regulation of epithelial cell differentiation mammary gland development positive regulation of natural killer cell differentiation negative regulation of mast cell apoptotic process T cell differentiation in thymus positive regulation of multicellular organism growth positive regulation of activated T cell proliferation T cell homeostasis negative regulation of apoptotic process response to peptide hormone positive regulation of interleukin-2 biosynthetic process positive regulation of B cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of lymphocyte differentiation negative regulation of erythrocyte differentiation positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter positive regulation of natural killer cell mediated cytotoxicity development of secondary female sexual characteristics development of secondary male sexual characteristics Peyer's patch development positive regulation of inflammatory response mammary gland involution positive regulation of mast cell differentiation prostate gland epithelium morphogenesis epithelial cell differentiation involved in prostate gland development mammary gland epithelium development positive regulation of mast cell proliferation cellular response to granulocyte macrophage colony-stimulating factor stimulus
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