Invitrogen

Phospho-STAT5 alpha (Tyr694) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

31 Published Figures

47 References

View all (104) STAT5 alpha antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Phospho-STAT5 alpha (Tyr694) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 35

Phospho-STAT5 alpha (Tyr694) Antibody (71-6900) in ICC/IF

Immunofluorescent analysis of STAT5 (pY694) was done on 70% confluent log phase HeLa cells; untreated & treated (serum starved for 16 hours followed by treatment with 20 ng/mL IFN alpha for 1 hour). The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with ... View More

Phospho-STAT5 alpha (Tyr694) Antibody in Western Blot (WB)

Phospho-STAT5 alpha (Tyr694) Antibody in Immunohistochemistry (IHC)

Phospho-STAT5 alpha (Tyr694) Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Phospho-STAT5 alpha (Tyr694) Antibody in Western Blot, ChIP Assay (WB, ChIP)

Phospho-STAT5 alpha (Tyr694) Antibody in ChIP Assay (ChIP)

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Product Details

71-6900

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 31 publications 31 publications

Immunohistochemistry (IHC)

Assay-dependent

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 4 publications 4 publications

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

View 1 publication 1 publication

ChIP assay (ChIP)

View 2 publications 2 publications

Gel Shift (GS)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Sheep, Rat

Published species

Hamster, Human, Mouse, Non-human primate, Rat, Tag, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic tyrosine-phosphorylated peptide encompassing the conserved C-terminal tyrosine phosphorylation site (Y694) of murine STAT5. This peptide is conserved in human, bovine, sheep, and rat STAT5 proteins

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533991

Target Information

STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5 alpha is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of STAT5 alpha is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells. STAT5 alpha, along with STAT5 beta, were identified in mouse. The amino acid sequences of STAT5 alpha and STAT5 beta show 96% sequence similarity, and both proteins are co expressed in most tissues in virgin and lactating mice. However, differential accumulation of STAT5 alpha and STAT5 beta mRNA has been reported for both muscle and mammary tissue. STAT5 alpha is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Occludin is a target of Src kinase and promotes lipid secretion by binding to BTN1a1 and XOR.

PLoS biology

Lu Y,Zhou T,Xu C,Wang R,Feng D,Li J,Wang X,Kong Y,Hu G,Kong X,Lu P

71-6900 was used in Western Blot to demonstrate that Ocln is a downstream target of Src kinase and promotes LD secretion by binding to BTN1a1 and XOR.

Sat Jan 01 00:00:00 EST 2022

STAT5 is Expressed in CD34⁺/CD38^- Stem Cells and Serves as a Potential Molecular Target in Ph-Negative Myeloproliferative Neoplasms.

Cancers

Hadzijusufovic E,Keller A,Berger D,Greiner G,Wingelhofer B,Witzeneder N,Ivanov D,Pecnard E,Nivarthi H,Schur FKM,Filik Y,Kornauth C,Neubauer HA,Müllauer L,Tin G,Park J,de Araujo ED,Gunning PT,Hoermann G,Gouilleux F,Kralovics R,Moriggl R,Valent P

71-6900 was used in Immunohistochemistry-immunofluorescence to show that CD34+/CD38- MPN-SC express pSTAT5 and that pSTAT5 is expressed in the nuclear and cytoplasmic compartment of MPN cells.

Tue Apr 21 00:00:00 EDT 2020

STAT5 is Expressed in CD34⁺/CD38^- Stem Cells and Serves as a Potential Molecular Target in Ph-Negative Myeloproliferative Neoplasms.

Cancers

71-6900 was used in Immunohistochemistry-immunofluorescence to show that CD34+/CD38- MPN-SC express pSTAT5 and that pSTAT5 is expressed in the nuclear and cytoplasmic compartment of MPN cells.

Tue Apr 21 00:00:00 EDT 2020

STAT5 is Expressed in CD34⁺/CD38^- Stem Cells and Serves as a Potential Molecular Target in Ph-Negative Myeloproliferative Neoplasms.

Cancers

71-6900 was used in Immunohistochemistry-immunofluorescence to show that CD34+/CD38- MPN-SC express pSTAT5 and that pSTAT5 is expressed in the nuclear and cytoplasmic compartment of MPN cells.

Tue Apr 21 00:00:00 EDT 2020

Brain leptin reduces liver lipids by increasing hepatic triglyceride secretion and lowering lipogenesis.

Nature communications

Hackl MT,Fürnsinn C,Schuh CM,Krssak M,Carli F,Guerra S,Freudenthaler A,Baumgartner-Parzer S,Helbich TH,Luger A,Zeyda M,Gastaldelli A,Buettner C,Scherer T

71-6900 was used in Western Blotting to study the underlying mechanism by which leptin reverses steatosis of hypoleptinemic patients.

Thu Jun 20 00:00:00 EDT 2019

STAT5BN642H is a driver mutation for T cell neoplasia.

The Journal of clinical investigation

Pham HTT,Maurer B,Prchal-Murphy M,Grausenburger R,Grundschober E,Javaheri T,Nivarthi H,Boersma A,Kolbe T,Elabd M,Halbritter F,Pencik J,Kazemi Z,Grebien F,Hengstschläger M,Kenner L,Kubicek S,Farlik M,Bock C,Valent P,Müller M,Rülicke T,Sexl V,Moriggl R

71-6900 was used in Western Blotting to examine the role of the Asp642His STAT5 mutation in driving T cell neoplasms.

Tue Jan 02 00:00:00 EST 2018

Elevated collagen-I augments tumor progressive signals, intravasation and metastasis of prolactin-induced estrogen receptor alpha positive mammary tumor cells.

Breast cancer research : BCR

Barcus CE,O'Leary KA,Brockman JL,Rugowski DE,Liu Y,Garcia N,Yu M,Keely PJ,Eliceiri KW,Schuler LA

71-6900 was used in immunohistochemistry - paraffin section and western blot to investigate estrogen receptor alpha positive breast cancer

Thu Jan 19 00:00:00 EST 2017

Histone H1 and Chromosomal Protein HMGN2 Regulate Prolactin-induced STAT5 Transcription Factor Recruitment and Function in Breast Cancer Cells.

The Journal of biological chemistry

Schauwecker SM,Kim JJ,Licht JD,Clevenger CV

716900 was used in western blot to determine the molecular mechanisms that enable JAK2/signal transducer and activator of transcription 5 to access promoters of genes implicated in breast cancer pathogenesis

Fri Feb 10 00:00:00 EST 2017

Histone H1 and Chromosomal Protein HMGN2 Regulate Prolactin-induced STAT5 Transcription Factor Recruitment and Function in Breast Cancer Cells.

The Journal of biological chemistry

Schauwecker SM,Kim JJ,Licht JD,Clevenger CV

Fri Feb 10 00:00:00 EST 2017

Direct targets of pSTAT5 signalling in erythropoiesis.

PloS one

Gillinder KR,Tuckey H,Bell CC,Magor GW,Huang S,Ilsley MD,Perkins AC

71-6900 was used in Chromatin immunoprecipitation to find erythropoietin-dependent pSTAT5 target genes in murine erythroid cells.

Wed Sep 27 00:00:00 EDT 2017

Identification of STAT3 and STAT5 proteins in the rat suprachiasmatic nucleus and the Day/Night difference in astrocytic STAT3 phosphorylation in response to lipopolysaccharide.

Journal of neuroscience research

Moravcová S,Červená K,Pačesová D,Bendová Z

71-6900 was used in immunohistochemistry (free floating) to examine the daily rhythm of STAT3 protein.

Fri Jan 01 00:00:00 EST 2016

Mycotoxin ochratoxin A disrupts renal development via a miR-731/prolactin receptor axis in zebrafish.

Toxicology research

Wu TS,Yang JJ,Wang YW,Yu FY,Liu BH

71-6900 was used in Western Blotting to study the molecular mechanisms associated with mycotoxin ochratoxin A toxicity in a developing organism.

Tue Mar 01 00:00:00 EST 2016

Prolactin signaling through focal adhesion complexes is amplified by stiff extracellular matrices in breast cancer cells.

Oncotarget

Barcus CE,Keely PJ,Eliceiri KW,Schuler LA

716900 was used in western blot to explore the extracellular matrix features that control prolactin signals

Tue Jul 26 00:00:00 EDT 2016

Common γ-chain cytokine signaling is required for macroautophagy induction during CD4+ T-cell activation.

Autophagy

Botbol Y,Patel B,Macian F

Published figure using Phospho-STAT5 alpha (Tyr694) polyclonal antibody (Product # 71-6900) in Western Blot

Tue Sep 13 00:00:00 EDT 2016

Cysteine induces longitudinal bone growth in mice by upregulating IGF-I.

International journal of molecular medicine

Moon PD,Kim MH,Oh HA,Nam SY,Han NR,Jeong HJ,Kim HM

71-6900 was used in Western Blotting to evaluate the effects of cysteine on longitudinal bone growth.

Sat Aug 01 00:00:00 EDT 2015

Role of STAT5 and epigenetics in lactation-associated upregulation of multidrug transporter ABCG2 in the mammary gland.

American journal of physiology. Endocrinology and metabolism

Wu AM,Yang M,Dalvi P,Turinsky AL,Wang W,Butcher D,Egan SE,Weksberg R,Harper PA,Ito S

71-6900 was used in immunohistochemistry - paraffin section and western blot to elucidate the molecular mechanisms involved in the upregulation of ABCG2 during lactation

Wed Oct 01 00:00:00 EDT 2014

Reciprocal occupancy of BCL6 and STAT5 on Growth Hormone target genes: contrasting transcriptional outcomes and promoter-specific roles of p300 and HDAC3.

Molecular and cellular endocrinology

Lin G,LaPensee CR,Qin ZS,Schwartz J

71-6900 was used in ChIP assay to study the regulation of growth hormone stimulated genes

Mon Sep 01 00:00:00 EDT 2014

Reciprocal occupancy of BCL6 and STAT5 on Growth Hormone target genes: contrasting transcriptional outcomes and promoter-specific roles of p300 and HDAC3.

Molecular and cellular endocrinology

Lin G,LaPensee CR,Qin ZS,Schwartz J

71-6900 was used in ChIP assay to study the regulation of growth hormone stimulated genes

Mon Sep 01 00:00:00 EDT 2014

Miz1 deficiency in the mammary gland causes a lactation defect by attenuated Stat5 expression and phosphorylation.

PloS one

Sanz-Moreno A,Fuhrmann D,Wolf E,von Eyss B,Eilers M,Elsässer HP

Published figure using Phospho-STAT5 alpha (Tyr694) polyclonal antibody (Product # 71-6900) in Immunohistochemistry

Sat Jan 10 00:00:00 EST 2015

Reliable quantification of protein expression and cellular localization in histological sections.

PloS one

Schlederer M,Mueller KM,Haybaeck J,Heider S,Huttary N,Rosner M,Hengstschläger M,Moriggl R,Dolznig H,Kenner L

71-6900 was used in immunohistochemistry (paraffin), knockout immunohistochemistry, knockout western blot, and western blot to report that image analysis decreased inter-observer variability when scoring immunohistologically stained human hepatocellular patient samples.

Wed Mar 04 00:00:00 EST 2015

Reliable quantification of protein expression and cellular localization in histological sections.

PloS one

Schlederer M,Mueller KM,Haybaeck J,Heider S,Huttary N,Rosner M,Hengstschläger M,Moriggl R,Dolznig H,Kenner L

Wed Mar 04 00:00:00 EST 2015

Induction of multidrug resistance transporter ABCG2 by prolactin in human breast cancer cells.

Molecular pharmacology

Wu AM,Dalvi P,Lu X,Yang M,Riddick DS,Matthews J,Clevenger CV,Ross DD,Harper PA,Ito S

71-6900 was used in western blot to elucidate the role of the lactogenic hormone prolactin in the regulation of ABCG2.

Fri Feb 01 00:00:00 EST 2013

Stiff collagen matrices increase tumorigenic prolactin signaling in breast cancer cells.

The Journal of biological chemistry

Barcus CE,Keely PJ,Eliceiri KW,Schuler LA

71-6900 was used in western blot to study the involvement of collagen matrices in tumorigenic prolactin signaling in breast cancer cells

Fri May 03 00:00:00 EDT 2013

A humanized pattern of aromatase expression is associated with mammary hyperplasia in mice.

Endocrinology

Zhao H,Pearson EK,Brooks DC,Coon JS,Chen D,Demura M,Zhang M,Clevenger CV,Xu X,Veenstra TD,Chatterton RT,DeMayo FJ,Bulun SE

Published figure using Phospho-STAT5 alpha (Tyr694) polyclonal antibody (Product # 71-6900) in Western Blot

Fri Jun 01 00:00:00 EDT 2012

Impairment of hepatic growth hormone and glucocorticoid receptor signaling causes steatosis and hepatocellular carcinoma in mice.

Hepatology (Baltimore, Md.)

Mueller KM,Kornfeld JW,Friedbichler K,Blaas L,Egger G,Esterbauer H,Hasselblatt P,Schlederer M,Haindl S,Wagner KU,Engblom D,Haemmerle G,Kratky D,Sexl V,Kenner L,Kozlov AV,Terracciano L,Zechner R,Schuetz G,Casanova E,Pospisilik JA,Heim MH,Moriggl R

71-6900 was used in western blot to determine the roles of growth hormone and STAT5 in metabolism and liver cancer onset.

Sat Oct 01 00:00:00 EDT 2011

Vascular smooth muscle Jak2 deletion prevents angiotensin II-mediated neointima formation following injury in mice.

Journal of molecular and cellular cardiology

Kirabo A,Oh SP,Kasahara H,Wagner KU,Sayeski PP

71-6900 was used in western blot to test if Jak2 contributes to Ang II-mediated neointima formation following vascular injury.

Wed Jun 01 00:00:00 EDT 2011

Stat5a serine 725 and 779 phosphorylation is a prerequisite for hematopoietic transformation.

Blood

Friedbichler K,Kerenyi MA,Kovacic B,Li G,Hoelbl A,Yahiaoui S,Sexl V,Müllner EW,Fajmann S,Cerny-Reiterer S,Valent P,Beug H,Gouilleux F,Bunting KD,Moriggl R

71-6900 was used in western blot to study the role of Stat5 serine phosphorylation in oncogenesis

Thu Sep 02 00:00:00 EDT 2010

Stat5a serine 725 and 779 phosphorylation is a prerequisite for hematopoietic transformation.

Blood

Friedbichler K,Kerenyi MA,Kovacic B,Li G,Hoelbl A,Yahiaoui S,Sexl V,Müllner EW,Fajmann S,Cerny-Reiterer S,Valent P,Beug H,Gouilleux F,Bunting KD,Moriggl R

71-6900 was used in western blot to study the role of Stat5 serine phosphorylation in oncogenesis

Thu Sep 02 00:00:00 EDT 2010

Disruption of the growth hormone--signal transducer and activator of transcription 5--insulinlike growth factor 1 axis severely aggravates liver fibrosis in a mouse model of cholestasis.

Hepatology (Baltimore, Md.)

Blaas L,Kornfeld JW,Schramek D,Musteanu M,Zollner G,Gumhold J,van Zijl F,Schneller D,Esterbauer H,Egger G,Mair M,Kenner L,Mikulits W,Eferl R,Moriggl R,Penninger J,Trauner M,Casanova E

71-6900 was used in western blot to determine the role of STAT5 in liver fibrogenesis

Thu Apr 01 00:00:00 EDT 2010

JAK2, but not Src family kinases, is required for STAT, ERK, and Akt signaling in response to growth hormone in preadipocytes and hepatoma cells.

Molecular endocrinology (Baltimore, Md.)

Jin H,Lanning NJ,Carter-Su C

Published figure using Phospho-STAT5 alpha (Tyr694) polyclonal antibody (Product # 71-6900) in Western Blot

Fri Aug 01 00:00:00 EDT 2008

Direct glucocorticoid receptor-Stat5 interaction in hepatocytes controls body size and maturation-related gene expression.

Genes & development

Engblom D,Kornfeld JW,Schwake L,Tronche F,Reimann A,Beug H,Hennighausen L,Moriggl R,Schütz G

Published figure using Phospho-STAT5 alpha (Tyr694) polyclonal antibody (Product # 71-6900) in Western Blot

Tue May 15 00:00:00 EDT 2007

STAT3 signaling is activated in human skeletal muscle following acute resistance exercise.

Journal of applied physiology (Bethesda, Md. : 1985)

Trenerry MK,Carey KA,Ward AC,Cameron-Smith D

71-6900 was used in western blot to examine STAT3 following resistance exercise in human skeletal muscle

Sun Apr 01 00:00:00 EDT 2007

Mammary epithelial-specific deletion of the focal adhesion kinase gene leads to severe lobulo-alveolar hypoplasia and secretory immaturity of the murine mammary gland.

The Journal of biological chemistry

Nagy T,Wei H,Shen TL,Peng X,Liang CC,Gan B,Guan JL

71-6900 was used in immunohistochemistry and western blot to assess the role of FAK in mammary epithelial cells in vivo

Fri Oct 26 00:00:00 EDT 2007

Mammary epithelial-specific deletion of the focal adhesion kinase gene leads to severe lobulo-alveolar hypoplasia and secretory immaturity of the murine mammary gland.

The Journal of biological chemistry

Nagy T,Wei H,Shen TL,Peng X,Liang CC,Gan B,Guan JL

71-6900 was used in immunohistochemistry and western blot to assess the role of FAK in mammary epithelial cells in vivo

Fri Oct 26 00:00:00 EDT 2007

Stat5 tetramer formation is associated with leukemogenesis.

Cancer cell

Moriggl R,Sexl V,Kenner L,Duntsch C,Stangl K,Gingras S,Hoffmeyer A,Bauer A,Piekorz R,Wang D,Bunting KD,Wagner EF,Sonneck K,Valent P,Ihle JN,Beug H

71-6900 was used in EMSA to propose that Stat5 tetramers are associated with leukemogenesis

Sat Jan 01 00:00:00 EST 2005

Glucocorticoid receptor function in hepatocytes is essential to promote postnatal body growth.

Genes & development

Tronche F,Opherk C,Moriggl R,Kellendonk C,Reimann A,Schwake L,Reichardt HM,Stangl K,Gau D,Hoeflich A,Beug H,Schmid W,Schütz G

Mon Mar 01 00:00:00 EST 2004

Stat5a is tyrosine phosphorylated and nuclear localized in a high proportion of human breast cancers.

International journal of cancer

Cotarla I,Ren S,Zhang Y,Gehan E,Singh B,Furth PA

71-6900 was used in immunohistochemistry - paraffin section to examine the expression, tyrosine phosphorylation status and nuclear localization of Stat5a in human breast cancers.

Fri Feb 20 00:00:00 EST 2004

Dendritic cell maturation requires STAT1 and is under feedback regulation by suppressors of cytokine signaling.

Journal of immunology (Baltimore, Md. : 1950)

Jackson SH,Yu CR,Mahdi RM,Ebong S,Egwuagu CE

Sun Feb 15 00:00:00 EST 2004

Dendritic cell maturation requires STAT1 and is under feedback regulation by suppressors of cytokine signaling.

Journal of immunology (Baltimore, Md. : 1950)

Jackson SH,Yu CR,Mahdi RM,Ebong S,Egwuagu CE

Sun Feb 15 00:00:00 EST 2004

Dendritic cell maturation requires STAT1 and is under feedback regulation by suppressors of cytokine signaling.

Journal of immunology (Baltimore, Md. : 1950)

Jackson SH,Yu CR,Mahdi RM,Ebong S,Egwuagu CE

Sun Feb 15 00:00:00 EST 2004

Growth hormone receptor expression and function in pituitary adenomas.

Clinical endocrinology

Clausen LR,Kristiansen MT,Rasmussen LM,Billestrup N,Blaabjerg O,Ledet T,Jørgensen JO

71-6900 was used in western blot to assess GH receptor expression and signal transduction and the effect of GH and IGF-I exposure on cell proliferation and hormone secretion in nonfunctioning pituitary adenomas

Sat May 01 00:00:00 EDT 2004

Caveolar and lipid raft localization of the growth hormone receptor and its signaling elements: impact on growth hormone signaling.

The Journal of biological chemistry

Yang N,Huang Y,Jiang J,Frank SJ

Fri May 14 00:00:00 EDT 2004

Distinct pathways involving the FK506-binding proteins 12 and 12.6 underlie IL-2-versus IL-15-mediated proliferation of T cells.

Proceedings of the National Academy of Sciences of the United States of America

Dubois S,Shou W,Haneline LS,Fleischer S,Waldmann TA,Müller JR

71-6900 was used in western blot to study T cell responses in the presence of IL-2 and IL-5.

Tue Nov 25 00:00:00 EST 2003

Essential functions of p21-activated kinase 1 in morphogenesis and differentiation of mammary glands.

The Journal of cell biology

Wang RA,Vadlamudi RK,Bagheri-Yarmand R,Beuvink I,Hynes NE,Kumar R

71-6900 was used in western blot to study the role of p21-activated kinase in the mammary gland.

Mon May 12 00:00:00 EDT 2003

Essential functions of p21-activated kinase 1 in morphogenesis and differentiation of mammary glands.

The Journal of cell biology

Wang RA,Vadlamudi RK,Bagheri-Yarmand R,Beuvink I,Hynes NE,Kumar R

71-6900 was used in western blot to study the role of p21-activated kinase in the mammary gland.

Mon May 12 00:00:00 EDT 2003

Essential functions of p21-activated kinase 1 in morphogenesis and differentiation of mammary glands.

The Journal of cell biology

Wang RA,Vadlamudi RK,Bagheri-Yarmand R,Beuvink I,Hynes NE,Kumar R

71-6900 was used in western blot to study the role of p21-activated kinase in the mammary gland.

Mon May 12 00:00:00 EDT 2003

Inhibition of prolactin (PRL)-induced proliferative signals in breast cancer cells by a molecular mimic of phosphorylated PRL, S179D-PRL.

Endocrinology

Schroeder MD,Brockman JL,Walker AM,Schuler LA

71-6900 was used in western blot to test if a mimic of phosphorylated human prolactin, S79D-PRL, initiates signaling in mammary tumor cells alone and in combination with unmodified prolactin.

Mon Dec 01 00:00:00 EST 2003

Dysregulation of mammary Stats 1,3 and 5 and PRL receptors by overexpression of TGFalpha.

Molecular and cellular endocrinology

Schroeder MD,Rose-Hellekant TA,Sandgren EP,Schuler LA

71-6900 was used in western blot to investigate the role of STATs and PRL receptors in TGFalpha-induced mammary epithelial abnormalities in vivo.

Wed Apr 25 00:00:00 EDT 2001

Activation of Stat3 in v-Src-transformed fibroblasts requires cooperation of Jak1 kinase activity.

The Journal of biological chemistry

Zhang Y,Turkson J,Carter-Su C,Smithgall T,Levitzki A,Kraker A,Krolewski JJ,Medveczky P,Jove R

Fri Aug 11 00:00:00 EDT 2000

Disulfide linkage of growth hormone (GH) receptors (GHR) reflects GH-induced GHR dimerization. Association of JAK2 with the GHR is enhanced by receptor dimerization.

The Journal of biological chemistry

Zhang Y,Jiang J,Kopchick JJ,Frank SJ

Fri Nov 12 00:00:00 EST 1999

Disulfide linkage of growth hormone (GH) receptors (GHR) reflects GH-induced GHR dimerization. Association of JAK2 with the GHR is enhanced by receptor dimerization.

The Journal of biological chemistry

Zhang Y,Jiang J,Kopchick JJ,Frank SJ

Fri Nov 12 00:00:00 EST 1999

Disulfide linkage of growth hormone (GH) receptors (GHR) reflects GH-induced GHR dimerization. Association of JAK2 with the GHR is enhanced by receptor dimerization.

The Journal of biological chemistry

Zhang Y,Jiang J,Kopchick JJ,Frank SJ

Fri Nov 12 00:00:00 EST 1999

Disulfide linkage of growth hormone (GH) receptors (GHR) reflects GH-induced GHR dimerization. Association of JAK2 with the GHR is enhanced by receptor dimerization.

The Journal of biological chemistry

Zhang Y,Jiang J,Kopchick JJ,Frank SJ

Fri Nov 12 00:00:00 EST 1999

Disulfide linkage of growth hormone (GH) receptors (GHR) reflects GH-induced GHR dimerization. Association of JAK2 with the GHR is enhanced by receptor dimerization.

The Journal of biological chemistry

Zhang Y,Jiang J,Kopchick JJ,Frank SJ

Fri Nov 12 00:00:00 EST 1999

Disulfide linkage of growth hormone (GH) receptors (GHR) reflects GH-induced GHR dimerization. Association of JAK2 with the GHR is enhanced by receptor dimerization.

The Journal of biological chemistry

Zhang Y,Jiang J,Kopchick JJ,Frank SJ

Fri Nov 12 00:00:00 EST 1999

Disulfide linkage of growth hormone (GH) receptors (GHR) reflects GH-induced GHR dimerization. Association of JAK2 with the GHR is enhanced by receptor dimerization.

The Journal of biological chemistry

Zhang Y,Jiang J,Kopchick JJ,Frank SJ

Fri Nov 12 00:00:00 EST 1999

Disulfide linkage of growth hormone (GH) receptors (GHR) reflects GH-induced GHR dimerization. Association of JAK2 with the GHR is enhanced by receptor dimerization.

The Journal of biological chemistry

Zhang Y,Jiang J,Kopchick JJ,Frank SJ

Fri Nov 12 00:00:00 EST 1999

Bioinformatics

Protein Aliases: Mammary gland factor; mammary gland factor STAT5A; signal transducer and activator of transcription 5; Signal transducer and activator of transcription 5A; signal transducer and activator of transcription factor 5A; STAT; STAT5A, Mammary Gland Factor; STAT5B

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Gene Aliases: AA959963; MGF; Mpf; STAT5; STAT5A; STAT5A/MGF; STATA5

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UniProt ID: (Human) P42229, (Sheep) P42231, (Bovine) Q95115, (Rat) Q62771, (Mouse) P42230

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Entrez Gene ID: (Human) 6776, (Sheep) 443419, (Bovine) 282375, (Rat) 24918, (Mouse) 20850

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Function(s)

DNA binding transcription factor activity, sequence-specific DNA binding protein tyrosine kinase activity signal transducer activity protein binding RNA polymerase II core promoter sequence-specific DNA binding double-stranded DNA binding protein phosphatase binding sequence-specific DNA binding DNA-binding transcription factor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-STAT5 alpha (Tyr694) Polyclonal Antibody

Phospho-STAT5 alpha (Tyr694) Antibody (71-6900) in ICC/IF

Product Details

71-6900

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Gel Shift (GS)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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