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Phospho-Splicing factor 1 (Ser82) Antibody (BS-17680R) in WB
Mouse embryo lysates probed with splicing factor 1 (Ser82) Polyclonal Antibody, Unconjugated (bs-17680R) at 1:300 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
Product Details
BS-17680R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthesised phosphopeptide derived from human splicing factor 1 around the phosphorylation site of Ser82.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Splicing factor 1 is a nuclear pre-mRNA splicing factor. The protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BBP; M ZFM1; Mammalian branch point-binding protein; Splicing factor 1; Transcription factor ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162
Gene Aliases: BBP; D11S636; MBBP; MZFM; SF1; WBP4; ZCCHC25; ZFM1; Zfp162; ZNF162
UniProt ID: (Human) Q15637
Entrez Gene ID: (Human) 7536, (Mouse) 22668, (Rat) 117855
spliceosomal complex assembly
mRNA 3'-splice site recognition
mRNA splicing, via spliceosome
transcription, DNA-templated
regulation of transcription, DNA-templated
male sex determination
Leydig cell differentiation
negative regulation of smooth muscle cell proliferation
regulation of steroid biosynthetic process
negative regulation of nucleic acid-templated transcription
mRNA processing
RNA splicing
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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