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Product Details
200-301-A45
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
5F6.F1
Immunogen
Anti-STAT5 phospho Y694 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Specific for mouse Stat5a protein phosphorylated at Y694. A BLAST analysis was used to suggest cross-reactivity with Stat5a from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Stat5a from other sources has not been determined.
Target Information
STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5 alpha is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of STAT5 alpha is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells. STAT5 alpha, along with STAT5 beta, were identified in mouse. The amino acid sequences of STAT5 alpha and STAT5 beta show 96% sequence similarity, and both proteins are co expressed in most tissues in virgin and lactating mice. However, differential accumulation of STAT5 alpha and STAT5 beta mRNA has been reported for both muscle and mammary tissue. STAT5 alpha is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Mammary gland factor; mammary gland factor STAT5A; signal transducer and activator of transcription 5; Signal transducer and activator of transcription 5A; STAT; STAT5B
Gene Aliases: AA959963; MGF; Mpf; STAT5; STAT5A
UniProt ID: (Human) P42229, (Rat) Q62771, (Mouse) P42230
Entrez Gene ID: (Human) 6776, (Rat) 24918, (Mouse) 20850
DNA binding
transcription factor activity, sequence-specific DNA binding
protein tyrosine kinase activity
signal transducer activity
protein binding
RNA polymerase II core promoter sequence-specific DNA binding
double-stranded DNA binding
protein phosphatase binding
sequence-specific DNA binding
DNA-binding transcription factor
allantoin metabolic process
positive regulation of endothelial cell proliferation
citrate metabolic process
2-oxoglutarate metabolic process
succinate metabolic process
oxaloacetate metabolic process
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
isoleucine metabolic process
valine metabolic process
creatine metabolic process
fatty acid metabolic process
JAK-STAT cascade
lactation
peptidyl-tyrosine phosphorylation
taurine metabolic process
prolactin signaling pathway
regulation of multicellular organism growth
positive regulation of blood vessel endothelial cell migration
creatinine metabolic process
JAK-STAT cascade involved in growth hormone signaling pathway
luteinization
natural killer cell differentiation
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
signal transduction
female pregnancy
positive regulation of cell proliferation
regulation of steroid metabolic process
cytokine-mediated signaling pathway
lipid storage
regulation of cell adhesion
regulation of epithelial cell differentiation
mammary gland development
response to nutrient levels
positive regulation of natural killer cell differentiation
negative regulation of mast cell apoptotic process
T cell differentiation in thymus
positive regulation of multicellular organism growth
positive regulation of activated T cell proliferation
T cell homeostasis
negative regulation of apoptotic process
response to peptide hormone
positive regulation of interleukin-2 biosynthetic process
response to ethanol
positive regulation of B cell differentiation
positive regulation of gamma-delta T cell differentiation
positive regulation of lymphocyte differentiation
negative regulation of erythrocyte differentiation
positive regulation of mitotic cell cycle
positive regulation of transcription from RNA polymerase II promoter
positive regulation of natural killer cell mediated cytotoxicity
development of secondary female sexual characteristics
development of secondary male sexual characteristics
Peyer's patch development
multicellular organismal reproductive process
positive regulation of inflammatory response
mammary gland involution
positive regulation of mast cell differentiation
prostate gland epithelium morphogenesis
epithelial cell differentiation involved in prostate gland development
mammary gland epithelium development
positive regulation of mast cell proliferation
cellular response to granulocyte macrophage colony-stimulating factor stimulus
Performance Guarantee
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