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Phospho-TFEB (Ser211) Antibody (PA5-114662) in ICC/IF
Immunocytochemistry analysis of Phospho-TFEB (Ser211) in A549 cells (4h of LPS treatment). Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114662). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjuga... View More
Product Details
PA5-114662
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TFEB around the phosphorylation site of Ser211.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2899298
Product Specific Information
Antibody detects endogenous levels of TFEB only when phosphorylated at Ser211.
Target Information
The Transcription factor EB (TFEB) is a member in the basic helix-loop-helix leucine zipper superfamily of transcription factors that is translocated in a subset of renal tumors. Recent studies have shown that lysosomal biogenesis is regulated by TFEB, which is in turn regulated by the mammalian target of rapamycin (mTOR) complex 1. Other evidence suggests that TFEB coordinates the major steps of the autophagic pathway by driving the expression of autophagy and lysosomal genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alpha TCFEB; bHLHe35; Class E basic helix-loop-helix protein 35; RP4-696P19.3; T-cell transcription factor EB; Transcription factor EB
Gene Aliases: ALPHATFEB; BHLHE35; TCFEB; TFEB
UniProt ID: (Human) P19484, (Mouse) Q9R210
Entrez Gene ID: (Human) 7942, (Rat) 316214, (Mouse) 21425
transcription factor activity, sequence-specific DNA binding
transcription regulatory region DNA binding
protein dimerization activity
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
protein heterodimerization activity
embryonic placenta development
adaptive immune response
transcription, DNA-templated
regulation of transcription, DNA-templated
autophagy
humoral immune response
lysosome organization
positive regulation of autophagy
positive regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
positive regulation of transcription, DNA-templated
immune system process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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