Invitrogen

Phospho-Tau (Ser214) Polyclonal Antibody

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This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

11 Published Figures

32 References

View all (188) Tau antibodies

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Datasheet

Protocols

Questions & Answers

Phospho-Tau (Ser214) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-Tau (Ser214) Antibody (44-742G) in ICC/IF

Immunofluorescent analysis of Phospho-Tau pSer214 Antibody was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Tau pSer214 Antibody (Product # 44-742G) at 1µg/mL in 1% BSA and incubated for 3 hours at ro... View More

Phospho-Tau (Ser214) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-Tau (Ser214) Antibody in Western Blot (WB)

Phospho-Tau (Ser214) Antibody in Immunohistochemistry (IHC)

Phospho-Tau (Ser214) Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Product Details

44-742G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 19 publications 19 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1 µg/mL

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Dot blot (DB)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Cat, Dog, Human, Mouse, Rat, Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Tau that contains serine 214. The sequence is conserved in many species including mouse, rat, rhesus monkey, baboon, cow and goat.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533740

Target Information

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

RNA methyltransferase NSun2 deficiency promotes neurodegeneration through epitranscriptomic regulation of tau phosphorylation.

Acta neuropathologica

Kim YA,Siddiqui T,Blaze J,Cosacak MI,Winters T,Kumar A,Tein E,Sproul AA,Teich AF,Bartolini F,Akbarian S,Kizil C,Hargus G,Santa-Maria I

44-742G was used in Western Blot to indicate that neuronal NSun2 deficiency promotes dysregulation of miR-125b and tau phosphorylation in AD and highlights a novel avenue for therapeutic targeting.

Sun Jan 01 00:00:00 EST 2023

Genome-wide association study and functional validation implicates JADE1 in tauopathy.

Acta neuropathologica

Farrell K,Kim S,Han N,Iida MA,Gonzalez EM,Otero-Garcia M,Walker JM,Richardson TE,Renton AE,Andrews SJ,Fulton-Howard B,Humphrey J,Vialle RA,Bowles KR,de Paiva Lopes K,Whitney K,Dangoor DK,Walsh H,Marcora E,Hefti MM,Casella A,Sissoko CT,Kapoor M,Novikova G,Udine E,Wong G,Tang W,Bhangale T,Hunkapiller J,Ayalon G,Graham RR,Cherry JD,Cortes EP,Borukov VY,McKee AC,Stein TD,Vonsattel JP,Teich AF,Gearing M,Glass J,Troncoso JC,Frosch MP,Hyman BT,Dickson DW,Murray ME,Attems J,Flanagan ME,Mao Q,Mesulam MM,Weintraub S,Woltjer RL,Pham T,Kofler J,Schneider JA,Yu L,Purohit DP,Haroutunian V,Hof PR,Gandy S,Sano M,Beach TG,Poon W,Kawas CH,Corrada MM,Rissman RA,Metcalf J,Shuldberg S,Salehi B,Nelson PT,Trojanowski JQ,Lee EB,Wolk DA,McMillan CT,Keene CD,Latimer CS,Montine TJ,Kovacs GG,Lutz MI,Fischer P,Perrin RJ,Cairns NJ,Franklin EE,Cohen HT,Raj T,Cobos I,Frost B,Goate A,White Iii CL,Crary JF

44-742G was used in Western Blotting to investigate if Primary age-related tauopathy has a genetic architecture that partially overlaps with Alzheimer disease and other tauopathies and suggests a novel role for JADE1 as a modifier of neurofibrillary degeneration.

Sat Jan 01 00:00:00 EST 2022

Enriched environment ameliorates propagation of tau pathology and improves cognition in rat model of tauopathy.

Frontiers in aging neuroscience

Mate V,Smolek T,Kazmerova ZV,Jadhav S,Brezovakova V,Jurkanin B,Uhrinova I,Basheer N,Zilka N,Katina S,Novak P

44-742G was used in Immunohistochemistry to show that EE seems to be able to slow down tau pathology progression, indicating the possible utility of similar interventions in early stages of AD where tangle loads are still low.

Tue Aug 16 00:00:00 EDT 2022

Tau antibody 77G7 targeting microtubule binding domain suppresses proteopathic tau to seed tau aggregation.

CNS neuroscience & therapeutics

Li L,Miao J,Chu D,Jin N,Tung YC,Dai CL,Hu W,Gong CX,Iqbal K,Liu F

44-742G was used in Immunohistochemistry and Western Blotting to indicate that 77G7 can effectively suppress the seeding activity of AD O-tau and thus could be developed as a potential immunotherapeutic drug to inhibit the propagation of tau pathology in AD and related tauopathies.

Thu Dec 01 00:00:00 EST 2022

Tau antibody 77G7 targeting microtubule binding domain suppresses proteopathic tau to seed tau aggregation.

CNS neuroscience & therapeutics

Li L,Miao J,Chu D,Jin N,Tung YC,Dai CL,Hu W,Gong CX,Iqbal K,Liu F

Thu Dec 01 00:00:00 EST 2022

cPKCγ Deficiency Exacerbates Autophagy Impairment and Hyperphosphorylated Tau Buildup through the AMPK/mTOR Pathway in Mice with Type 1 Diabetes Mellitus.

Neuroscience bulletin

Zheng J,Wang Y,Liu Y,Han S,Zhang Y,Luo Y,Yan Y,Li J,Zhao L

44-742G was used in Western Blot to indicate that cPKCγ promotes autophagy through the AMPK/mTOR signaling pathway, thus reducing the level of phosphorylated Tau at Ser214 and neurofibrillary tangles.

Sat Oct 01 00:00:00 EDT 2022

Dephosphorylation Passivates the Seeding Activity of Oligomeric Tau Derived From Alzheimer's Brain.

Frontiers in molecular neuroscience

Wu R,Li L,Shi R,Zhou Y,Jin N,Gu J,Tung YC,Liu F,Chu D

Published figure using Phospho-Tau (Ser214) polyclonal antibody (Product # 44-742G) in Western Blot

Tue Jun 01 00:00:00 EDT 2021

TBK1 interacts with tau and enhances neurodegeneration in tauopathy.

The Journal of biological chemistry

Abreha MH,Ojelade S,Dammer EB,McEachin ZT,Duong DM,Gearing M,Bassell GJ,Lah JJ,Levey AI,Shulman JM,Seyfried NT

44-742G was used in Western Blot to suggest that increased TBK1 activation may promote tau hyperphosphorylation and neuronal loss in AD and related tauopathies.

Tue Aug 31 00:00:00 EDT 2021

β-amyloid and tau pathology in the aging feline brain.

The Journal of comparative neurology

Fiock KL,Smith JD,Crary JF,Hefti MM

44-742G was used in Immunohistochemistry (Paraffin) to propose that the development of β-amyloid and tau pathology in ageing domestic cats is similar to human Alzheimer's disease and could serve as a model.

Wed Jan 01 00:00:00 EST 2020

Truncation of Tau selectively facilitates its pathological activities.

The Journal of biological chemistry

Gu J,Xu W,Jin N,Li L,Zhou Y,Chu D,Gong CX,Iqbal K,Liu F

44-742G was used in Western Blotting to suggest that the first 150 aa and the last 50 aa protect Tau from pathological characteristics and that their deletions facilitate pathological activities.

Fri Oct 02 00:00:00 EDT 2020

Pathological Tau From Alzheimer's Brain Induces Site-Specific Hyperphosphorylation and SDS- and Reducing Agent-Resistant Aggregation of Tau in vivo.

Frontiers in aging neuroscience

Miao J,Shi R,Li L,Chen F,Zhou Y,Tung YC,Hu W,Gong CX,Iqbal K,Liu F

44-742G was used in Western Blotting to speculate that AD P-tau seeds hyperphosphorylated tau to form aggregates, which resist to the dephosphorylation by PP2A, resulting in hyperphosphorylation and pathology of tau.

Thu Oct 01 00:00:00 EDT 2020

Tau-Mediated Disruption of the Spliceosome Triggers Cryptic RNA Splicing and Neurodegeneration in Alzheimer's Disease.

Cell reports

Hsieh YC,Guo C,Yalamanchili HK,Abreha M,Al-Ouran R,Li Y,Dammer EB,Lah JJ,Levey AI,Bennett DA,De Jager PL,Seyfried NT,Liu Z,Shulman JM

Published figure using Phospho-Tau (Ser214) polyclonal antibody (Product # 44-742G) in Immunohistochemistry

Tue Oct 08 00:00:00 EDT 2019

GSKIP-Mediated Anchoring Increases Phosphorylation of Tau by PKA but Not by GSK3beta via cAMP/PKA/GSKIP/GSK3/Tau Axis Signaling in Cerebrospinal Fluid and iPS Cells in Alzheimer Disease.

Journal of clinical medicine

Ko HJ,Chiou SJ,Wong YH,Wang YH,Lai Y,Chou CH,Wang C,Loh JK,Lieu AS,Cheng JT,Lin YT,Lu PJ,Fann MJ,Huang CF,Hong YR

Published figure using Phospho-Tau (Ser214) polyclonal antibody (Product # 44-742G) in Western Blot

Mon Oct 21 00:00:00 EDT 2019

Subacute to chronic Alzheimer-like alterations after controlled cortical impact in human tau transgenic mice.

Scientific reports

Zhang Y,Wu F,Iqbal K,Gong CX,Hu W,Liu F

44-742G was used in Western Blot to characterise Alzheimer-like alterations behaviourally, biochemically and immunohistochemically 6 weeks and 7 months after unilateral mild-to-moderate controlled cortical impact in human tau transgenic mice.

Thu Mar 07 00:00:00 EST 2019

Relevance of Phosphorylation and Truncation of Tau to the Etiopathogenesis of Alzheimer's Disease.

Frontiers in aging neuroscience

Zhou Y,Shi J,Chu D,Hu W,Guan Z,Gong CX,Iqbal K,Liu F

Published figure using Phospho-Tau (Ser214) polyclonal antibody (Product # 44-742G) in Western Blot

Sat Apr 09 00:00:00 EDT 2022

Expression of Tau Pathology-Related Proteins in Different Brain Regions: A Molecular Basis of Tau Pathogenesis.

Frontiers in aging neuroscience

Hu W,Wu F,Zhang Y,Gong CX,Iqbal K,Liu F

44-742G was used in Dot blot to provide the regional expression profiles of tau and tau pathology-related proteins in the brain.

Thu Oct 01 00:00:00 EDT 2020

Liraglutide Improves Water Maze Learning and Memory Performance While Reduces Hyperphosphorylation of Tau and Neurofilaments in APP/PS1/Tau Triple Transgenic Mice.

Neurochemical research

Chen S,Sun J,Zhao G,Guo A,Chen Y,Fu R,Deng Y

44742G was used in western blot to elucidate how liraglutide affects disease and cognitive function in a mouse model of Alzheimer disease

Tue Aug 01 00:00:00 EDT 2017

Tau-driven 26S proteasome impairment and cognitive dysfunction can be prevented early in disease by activating cAMP-PKA signaling.

Nature medicine

Myeku N,Clelland CL,Emrani S,Kukushkin NV,Yu WH,Goldberg AL,Duff KE

44-742G was used in western blot to research how cAMP-PKA signaling activation can prevent tau-driven 26S proteasome impairment and cognitive dysfunction

Fri Jan 01 00:00:00 EST 2016

Human Truncated Tau Induces Mature Neurofibrillary Pathology in a Mouse Model of Human Tauopathy.

Journal of Alzheimer's disease : JAD

Zimova I,Brezovakova V,Hromadka T,Weisova P,Cubinkova V,Valachova B,Filipcik P,Jadhav S,Smolek T,Novak M,Zilka N

44742G was used in immunohistochemistry - paraffin section to describe the molecular and behavioral features of R3m/4 transgenic mice expressing human non-mutated truncated tau protein and assess these mice as a model for Alzheimer's disease

Tue Sep 06 00:00:00 EDT 2016

Tau hyperphosphorylation in synaptosomes and neuroinflammation are associated with canine cognitive impairment.

The Journal of comparative neurology

Smolek T,Madari A,Farbakova J,Kandrac O,Jadhav S,Cente M,Brezovakova V,Novak M,Zilka N

44-742G was used in immunohistochemistry - paraffin section and western blot to study canine cognitive impairment syndrome.

Tue Mar 01 00:00:00 EST 2016

Tau hyperphosphorylation in synaptosomes and neuroinflammation are associated with canine cognitive impairment.

The Journal of comparative neurology

Smolek T,Madari A,Farbakova J,Kandrac O,Jadhav S,Cente M,Brezovakova V,Novak M,Zilka N

44-742G was used in immunohistochemistry - paraffin section and western blot to study canine cognitive impairment syndrome.

Tue Mar 01 00:00:00 EST 2016

Tau hyperphosphorylation in synaptosomes and neuroinflammation are associated with canine cognitive impairment.

The Journal of comparative neurology

Smolek T,Madari A,Farbakova J,Kandrac O,Jadhav S,Cente M,Brezovakova V,Novak M,Zilka N

44-742G was used in immunohistochemistry - paraffin section and western blot to study canine cognitive impairment syndrome.

Tue Mar 01 00:00:00 EST 2016

Hypersialylation is a common feature of neurofibrillary tangles and granulovacuolar degenerations in Alzheimer's disease and tauopathy brains.

Neuropathology : official journal of the Japanese Society of Neuropathology

Nagamine S,Yamazaki T,Makioka K,Fujita Y,Ikeda M,Takatama M,Okamoto K,Yokoo H,Ikeda Y

44-742G was used in Immunohistochemistry to study the role of sialylation in the accumulation of phosphorylated-tau in Alzheimer's disease and other tauopathies.

Mon Aug 01 00:00:00 EDT 2016

Rapid alteration of protein phosphorylation during postmortem: implication in the study of protein phosphorylation.

Scientific reports

Wang Y,Zhang Y,Hu W,Xie S,Gong CX,Iqbal K,Liu F

44-742G was used in western blot to characterize postmortem behavior and the rapid alteration of protein phosphorylation

Thu Oct 29 00:00:00 EDT 2015

Age-dependent effects of A53T alpha-synuclein on behavior and dopaminergic function.

PloS one

Oaks AW,Frankfurt M,Finkelstein DI,Sidhu A

Published figure using Phospho-Tau (Ser214) polyclonal antibody (Product # 44-742G) in Western Blot

Mon Sep 16 00:00:00 EDT 2013

Pseudomonas aeruginosa exotoxin Y is a promiscuous cyclase that increases endothelial tau phosphorylation and permeability.

The Journal of biological chemistry

Ochoa CD,Alexeyev M,Pastukh V,Balczon R,Stevens T

Published figure using Phospho-Tau (Ser214) polyclonal antibody (Product # 44-742G) in Immunoprecipitation

Fri Jul 20 00:00:00 EDT 2012

Pseudomonas aeruginosa exotoxin Y is a promiscuous cyclase that increases endothelial tau phosphorylation and permeability.

The Journal of biological chemistry

Ochoa CD,Alexeyev M,Pastukh V,Balczon R,Stevens T

Published figure using Phospho-Tau (Ser214) polyclonal antibody (Product # 44-742G) in Immunoprecipitation

Fri Jul 20 00:00:00 EDT 2012

Pseudomonas aeruginosa exotoxin Y is a promiscuous cyclase that increases endothelial tau phosphorylation and permeability.

The Journal of biological chemistry

Ochoa CD,Alexeyev M,Pastukh V,Balczon R,Stevens T

Published figure using Phospho-Tau (Ser214) polyclonal antibody (Product # 44-742G) in Immunoprecipitation

Fri Jul 20 00:00:00 EDT 2012

The carboxy-terminal fragment of inhibitor-2 of protein phosphatase-2A induces Alzheimer disease pathology and cognitive impairment.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Wang X,Blanchard J,Kohlbrenner E,Clement N,Linden RM,Radu A,Grundke-Iqbal I,Iqbal K

Mon Nov 01 00:00:00 EDT 2010

The carboxy-terminal fragment of inhibitor-2 of protein phosphatase-2A induces Alzheimer disease pathology and cognitive impairment.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Wang X,Blanchard J,Kohlbrenner E,Clement N,Linden RM,Radu A,Grundke-Iqbal I,Iqbal K

Mon Nov 01 00:00:00 EDT 2010

Developmental regulation of tau phosphorylation, tau kinases, and tau phosphatases.

Journal of neurochemistry

Yu Y,Run X,Liang Z,Li Y,Liu F,Liu Y,Iqbal K,Grundke-Iqbal I,Gong CX

Sun Mar 01 00:00:00 EST 2009

Developmental regulation of tau phosphorylation, tau kinases, and tau phosphatases.

Journal of neurochemistry

Yu Y,Run X,Liang Z,Li Y,Liu F,Liu Y,Iqbal K,Grundke-Iqbal I,Gong CX

Sun Mar 01 00:00:00 EST 2009

Tumor-suppressor PTEN affects tau phosphorylation, aggregation, and binding to microtubules.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhang X,Li F,Bulloj A,Zhang YW,Tong G,Zhang Z,Liao FF,Xu H

44-742G was used in immunohistochemistry and western blot to study affects of tau phosphorylation, aggregation, and binding to microtubules by tumor-suppressor PTEN

Thu Jun 01 00:00:00 EDT 2006

Pseudophosphorylation of tau protein alters its ability for self-aggregation.

Journal of neurochemistry

Haase C,Stieler JT,Arendt T,Holzer M

Mon Mar 01 00:00:00 EST 2004

Pseudophosphorylation of tau protein alters its ability for self-aggregation.

Journal of neurochemistry

Haase C,Stieler JT,Arendt T,Holzer M

Mon Mar 01 00:00:00 EST 2004

Interaction of tau isoforms with Alzheimer's disease abnormally hyperphosphorylated tau and in vitro phosphorylation into the disease-like protein.

The Journal of biological chemistry

Alonso AD,Zaidi T,Novak M,Barra HS,Grundke-Iqbal I,Iqbal K

Fri Oct 12 00:00:00 EDT 2001

Bioinformatics

Protein Aliases: FLJ31424; FTDP17; G protein beta1/gamma2 subunit-interacting factor 1; map tau; MGC138549; Microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; microtubules; Neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; Paired helical filament-tau; PHF-tau; PHFtau; protein phosphatase 1, regulatory subunit 103; Tau microtubule-associated protein; Tau-4; Tau5

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Gene Aliases: AI413597; AW045860; DDPAC; FTDP-17; MAPT; MAPTL; MSTD; Mtapt; MTBT1; MTBT2; PPND; PPP1R103; pTau; RNPTAU; TAU

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UniProt ID: (Human) P10636, (Mouse) P10637

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Entrez Gene ID: (Human) 4137, (Rat) 29477, (Mouse) 17762

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Function(s)

structural constituent of cytoskeleton protein binding microtubule binding SH3 domain binding enzyme binding protein kinase binding protein complex binding apolipoprotein binding protein phosphatase 2A binding lipoprotein particle binding protein domain specific binding tubulin binding

Process(es)

microtubule cytoskeleton organization neuron migration brain development female pregnancy response to nutrient adult walking behavior axo-dendritic transport intrinsic apoptotic signaling pathway in response to oxidative stress response to organic substance regulation of autophagy regulation of microtubule polymerization positive regulation of microtubule polymerization negative regulation of intracellular transport positive regulation of axon extension mitochondrion transport along microtubule axon extension generation of neurons regulation of microtubule-based movement apoptotic process microtubule-based process axonogenesis neuron differentiation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-Tau (Ser214) Polyclonal Antibody

Phospho-Tau (Ser214) Antibody (44-742G) in ICC/IF

Product Details

44-742G

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Dot blot (DB)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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