Invitrogen

Phospho-Tau (Ser422) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

20 Published Figures

28 References

View all (188) Tau antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

Phospho-Tau (Ser422) Antibody in Western Blot (WB)

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Phospho-Tau (Ser422) Antibody (44-764G) in WB

Up-regulation and Antibody-Peptide Competition. Human recombinant Tau stimulated with GSK-3β (1 µg per µg Tau) for 45 minutes was added to background extracts, resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature and either left untreated (1-4) or treated with lambda phosphatase (5), then incubated with th... View More

Phospho-Tau (Ser422) Antibody in Immunocytochemistry (ICC/IF)

Phospho-Tau (Ser422) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-Tau (Ser422) Antibody in Immunohistochemistry (IHC)

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Product Details

44-764G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 3 publications 3 publications

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

ELISA (ELISA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Tau that contains serine 422. The sequence is conserved in mouse and rat.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533748

Target Information

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Methyl donor supplementation reduces phospho-Tau, Fyn and demethylated protein phosphatase 2A levels and mitigates learning and motor deficits in a mouse model of tauopathy.

Neuropathology and applied neurobiology

van Hummel A,Taleski G,Sontag JM,Feiten AF,Ke YD,Ittner LM,Sontag E

Published figure using Phospho-Tau (Ser422) polyclonal antibody (Product # 44-764G) in Western Blot

Tue Aug 01 00:00:00 EDT 2023

Herpes Simplex Virus Type 1 Induces AD-like Neurodegeneration Markers in Human Progenitor and Differentiated ReNcell VM Cells.

Microorganisms

Salgado B,Sastre I,Bullido MJ,Aldudo J

Published figure using Phospho-Tau (Ser422) polyclonal antibody (Product # 44-764G) in Immunocytochemistry

Thu May 04 00:00:00 EDT 2023

Herpes Simplex Virus Type 1 Induces AD-like Neurodegeneration Markers in Human Progenitor and Differentiated ReNcell VM Cells.

Microorganisms

Salgado B,Sastre I,Bullido MJ,Aldudo J

Published figure using Phospho-Tau (Ser422) polyclonal antibody (Product # 44-764G) in Immunocytochemistry

Thu May 04 00:00:00 EDT 2023

Herpes Simplex Virus Type 1 Induces AD-like Neurodegeneration Markers in Human Progenitor and Differentiated ReNcell VM Cells.

Microorganisms

Salgado B,Sastre I,Bullido MJ,Aldudo J

Published figure using Phospho-Tau (Ser422) polyclonal antibody (Product # 44-764G) in Immunocytochemistry

Thu May 04 00:00:00 EDT 2023

Herpes Simplex Virus Type 1 Induces AD-like Neurodegeneration Markers in Human Progenitor and Differentiated ReNcell VM Cells.

Microorganisms

Salgado B,Sastre I,Bullido MJ,Aldudo J

Published figure using Phospho-Tau (Ser422) polyclonal antibody (Product # 44-764G) in Immunocytochemistry

Thu May 04 00:00:00 EDT 2023

Tau antibody 77G7 targeting microtubule binding domain suppresses proteopathic tau to seed tau aggregation.

CNS neuroscience & therapeutics

Li L,Miao J,Chu D,Jin N,Tung YC,Dai CL,Hu W,Gong CX,Iqbal K,Liu F

44-764G was used in Immunohistochemistry to indicate that 77G7 can effectively suppress the seeding activity of AD O-tau and thus could be developed as a potential immunotherapeutic drug to inhibit the propagation of tau pathology in AD and related tauopathies.

Thu Dec 01 00:00:00 EST 2022

Tau antibody 77G7 targeting microtubule binding domain suppresses proteopathic tau to seed tau aggregation.

CNS neuroscience & therapeutics

Li L,Miao J,Chu D,Jin N,Tung YC,Dai CL,Hu W,Gong CX,Iqbal K,Liu F

Thu Dec 01 00:00:00 EST 2022

Microglia phenotypes are associated with subregional patterns of concomitant tau, amyloid-β and α-synuclein pathologies in the hippocampus of patients with Alzheimer's disease and dementia with Lewy bodies.

Acta neuropathologica communications

Fixemer S,Ameli C,Hammer G,Salamanca L,Uriarte Huarte O,Schwartz C,Gérardy JJ,Mechawar N,Skupin A,Mittelbronn M,Bouvier DS

44-764G was used in Immunohistochemistry to suggest that combinations and severity of subregional pTau, Aβ and pSyn pathologies transform local microglia phenotypic composition in the hippocampus.

Wed Mar 16 00:00:00 EDT 2022

Amyloid-beta and tau pathologies act synergistically to induce novel disease stage-specific microglia subtypes.

Molecular neurodegeneration

Kim DW,Tu KJ,Wei A,Lau AJ,Gonzalez-Gil A,Cao T,Braunstein K,Ling JP,Troncoso JC,Wong PC,Blackshaw S,Schnaar RL,Li T

44-764G was used in Western Blot, Immunohistochemistry to suggest that both Aβ and tau pathologies are required for the disease stage-specific induction of early-stage AD-Associated Microglia (EADAM) and late-stage AD-Associated Microglia (LADAM), and reveal Siglecs as biomarkers of Alzheimer's disease progression.

Sat Dec 17 00:00:00 EST 2022

Alzheimer's disease brain-derived extracellular vesicles spread tau pathology in interneurons.

Brain : a journal of neurology

Ruan Z,Pathak D,Venkatesan Kalavai S,Yoshii-Kitahara A,Muraoka S,Bhatt N,Takamatsu-Yukawa K,Hu J,Wang Y,Hersh S,Ericsson M,Gorantla S,Gendelman HE,Kayed R,Ikezu S,Luebke JI,Ikezu T

44-764G was used in Immunocytochemistry to propose a novel mechanism for the spread of tau in hippocampal GABAergic interneurons via brain-derived extracellular vesicles and their subsequent neuronal dysfunction.

Fri Feb 12 00:00:00 EST 2021

Early detection of Alzheimer's disease using creatine chemical exchange saturation transfer magnetic resonance imaging.

NeuroImage

Chen L,van Zijl PCM,Wei Z,Lu H,Duan W,Wong PC,Li T,Xu J

44-764G was used in Immunohistochemistry to investigate if reduced creatine chemical exchange saturation transfer (CrCEST) contrast has the potential to serve as a new biomarker for early detection of Alzheimer''s disease.

Sun Aug 01 00:00:00 EDT 2021

Early detection of Alzheimer's disease using creatine chemical exchange saturation transfer magnetic resonance imaging.

NeuroImage

Chen L,van Zijl PCM,Wei Z,Lu H,Duan W,Wong PC,Li T,Xu J

Sun Aug 01 00:00:00 EDT 2021

Mixed pathologies in pancreatic β cells from subjects with neurodegenerative diseases and their interaction with prion protein.

Acta neuropathologica communications

Martinez-Valbuena I,Valenti-Azcarate R,Amat-Villegas I,Marcilla I,Marti-Andres G,Caballero MC,Riverol M,Tuñon MT,Fraser PE,Luquin MR

Published figure using Phospho-Tau (Ser422) polyclonal antibody (Product # 44-764G) in Immunohistochemistry

Thu Apr 08 00:00:00 EDT 2021

Mixed pathologies in pancreatic β cells from subjects with neurodegenerative diseases and their interaction with prion protein.

Acta neuropathologica communications

Martinez-Valbuena I,Valenti-Azcarate R,Amat-Villegas I,Marcilla I,Marti-Andres G,Caballero MC,Riverol M,Tuñon MT,Fraser PE,Luquin MR

Published figure using Phospho-Tau (Ser422) polyclonal antibody (Product # 44-764G) in Immunohistochemistry

Thu Apr 08 00:00:00 EDT 2021

Estrogens Inhibit Amyloid-β-Mediated Paired Helical Filament-Like Conformation of Tau Through Antioxidant Activity and miRNA 218 Regulation in hTau Mice.

Journal of Alzheimer's disease : JAD

Guglielmotto M,Manassero G,Vasciaveo V,Venezia M,Tabaton M,Tamagno E

44-764G was used in Western Blot to find that biological sex influences the effect of amyloid-β42 (Aβ42) monomers on pathological tau conformational change.

Thu Sep 09 00:00:00 EDT 2021

Amylin as a potential link between type 2 diabetes and alzheimer disease.

Annals of neurology

Martinez-Valbuena I,Valenti-Azcarate R,Amat-Villegas I,Riverol M,Marcilla I,de Andrea CE,Sánchez-Arias JA,Del Mar Carmona-Abellan M,Marti G,Erro ME,Martínez-Vila E,Tuñon MT,Luquin MR

44-764G was used in Western Blot, Immunohistochemistry to assess ed whether tau and β-amyloid (Aβ) deposits might be present in pancreatic tissue of subjects with Alzheimer's disease, and whether amylin, an amyloidogenic protein deposited in the pancreas of T2DM patients, might accumulate in the brain of Alzheimer's disease patients.

Tue Oct 01 00:00:00 EDT 2019

Amylin as a potential link between type 2 diabetes and alzheimer disease.

Annals of neurology

Martinez-Valbuena I,Valenti-Azcarate R,Amat-Villegas I,Riverol M,Marcilla I,de Andrea CE,Sánchez-Arias JA,Del Mar Carmona-Abellan M,Marti G,Erro ME,Martínez-Vila E,Tuñon MT,Luquin MR

Tue Oct 01 00:00:00 EDT 2019

Pretangle pathology within cholinergic nucleus basalis neurons coincides with neurotrophic and neurotransmitter receptor gene dysregulation during the progression of Alzheimer's disease.

Neurobiology of disease

Tiernan CT,Ginsberg SD,He B,Ward SM,Guillozet-Bongaarts AL,Kanaan NM,Mufson EJ,Counts SE

44-764G was used in Immunohistochemistry to study the molecular and cellular relationships between the evolution of tau pathology and nucleus basalis of Meynert neuronal survival.

Sat Sep 01 00:00:00 EDT 2018

Functional screening of Alzheimer risk loci identifies PTK2B as an in vivo modulator and early marker of Tau pathology.

Molecular psychiatry

Dourlen P,Fernandez-Gomez FJ,Dupont C,Grenier-Boley B,Bellenguez C,Obriot H,Caillierez R,Sottejeau Y,Chapuis J,Bretteville A,Abdelfettah F,Delay C,Malmanche N,Soininen H,Hiltunen M,Galas MC,Amouyel P,Sergeant N,Buée L,Lambert JC,Dermaut B

44-764G was used in western blot to identify PTK2B as an in vivo modulator and early marker of Tau pathology due to functional screening of Alzheimer risk loci

Thu Jun 01 00:00:00 EDT 2017

Rab7A regulates tau secretion.

Journal of neurochemistry

Rodriguez L,Mohamed NV,Desjardins A,Lippé R,Fon EA,Leclerc N

Published figure using Phospho-Tau (Ser422) polyclonal antibody (Product # 44-764G) in Western Blot

Mon May 01 00:00:00 EDT 2017

Beta-amyloid 1-42 monomers, but not oligomers, produce PHF-like conformation of Tau protein.

Aging cell

Manassero G,Guglielmotto M,Zamfir R,Borghi R,Colombo L,Salmona M,Perry G,Odetti P,Arancio O,Tamagno E,Tabaton M

44-764G was used in western blot elucidate the mechanistic relationship between amyloid beta1-42 and Tau

Sat Oct 01 00:00:00 EDT 2016

The neuritic plaque facilitates pathological conversion of tau in an Alzheimer's disease mouse model.

Nature communications

Li T,Braunstein KE,Zhang J,Lau A,Sibener L,Deeble C,Wong PC

Published figure using Phospho-Tau (Ser422) polyclonal antibody (Product # 44-764G) in Immunohistochemistry

Mon Jul 04 00:00:00 EDT 2016

High Resolution Dissection of Reactive Glial Nets in Alzheimer's Disease.

Scientific reports

Bouvier DS,Jones EV,Quesseveur G,Davoli MA,A Ferreira T,Quirion R,Mechawar N,Murai KK

44-764G was used in immunohistochemistry - free floating to analyze reactive glial nets in Alzheimer's disease by high resolution dissection

Tue Apr 19 00:00:00 EDT 2016

Neuronal uptake and propagation of a rare phosphorylated high-molecular-weight tau derived from Alzheimer's disease brain.

Nature communications

Takeda S,Wegmann S,Cho H,DeVos SL,Commins C,Roe AD,Nicholls SB,Carlson GA,Pitstick R,Nobuhara CK,Costantino I,Frosch MP,Müller DJ,Irimia D,Hyman BT

44-764G was used in western blot to research Alzheimer's disease brain and neuronal uptake and propagation of a rare phosphorylated high-molecular-weight tau

Tue Oct 13 00:00:00 EDT 2015

Reelin immunoreactivity in neuritic varicosities in the human hippocampal formation of non-demented subjects and Alzheimer's disease patients.

Acta neuropathologica communications

Notter T,Knuesel I

44-764G was used in Immunohistochemistry on paraffin embedded tissues-immunofluorescence to investigate whether a Reelin-associated neuropathology is observed in the aged human hippocampus and whether it correlated with dementia status.

Wed Jun 26 00:00:00 EDT 2013

Methylthioninium chloride (methylene blue) induces autophagy and attenuates tauopathy in vitro and in vivo.

Autophagy

Congdon EE,Wu JW,Myeku N,Figueroa YH,Herman M,Marinec PS,Gestwicki JE,Dickey CA,Yu WH,Duff KE

44-764G was used in western blot to report that autophagy is a mechanism by which methylene blue reduces tau levels.

Sun Apr 01 00:00:00 EDT 2012

Targeting phospho-Ser422 by active Tau Immunotherapy in the THYTau22 mouse model: a suitable therapeutic approach.

Current Alzheimer research

Troquier L,Caillierez R,Burnouf S,Fernandez-Gomez FJ,Grosjean ME,Zommer N,Sergeant N,Schraen-Maschke S,Blum D,Buee L

Published figure using Phospho-Tau (Ser422) polyclonal antibody (Product # 44-764G) in Immunohistochemistry

Tue May 01 00:00:00 EDT 2012

Targeting phospho-Ser422 by active Tau Immunotherapy in the THYTau22 mouse model: a suitable therapeutic approach.

Current Alzheimer research

Troquier L,Caillierez R,Burnouf S,Fernandez-Gomez FJ,Grosjean ME,Zommer N,Sergeant N,Schraen-Maschke S,Blum D,Buee L

Published figure using Phospho-Tau (Ser422) polyclonal antibody (Product # 44-764G) in Immunohistochemistry

Tue May 01 00:00:00 EDT 2012

Increased tau phosphorylation and cleavage in mouse models of type 1 and type 2 diabetes.

Endocrinology

Kim B,Backus C,Oh S,Hayes JM,Feldman EL

44-764G was used in immunohistochemistry and western blot to assess tau modification in type 1 and type 2 mouse models of diabetes

Tue Dec 01 00:00:00 EST 2009

Nature of "Tau" immunoreactivity in normal myonuclei and inclusion body myositis.

Muscle & nerve

Salajegheh M,Pinkus JL,Nazareno R,Amato AA,Parker KC,Greenberg SA

Thu Oct 01 00:00:00 EDT 2009

Increased tau phosphorylation on mitogen-activated protein kinase consensus sites and cognitive decline in transgenic models for Alzheimer's disease and FTDP-17: evidence for distinct molecular processes underlying tau abnormalities.

Molecular and cellular biology

Lambourne SL,Sellers LA,Bush TG,Choudhury SK,Emson PC,Suh YH,Wilkinson LS

Sat Jan 01 00:00:00 EST 2005

Pseudophosphorylation of tau protein alters its ability for self-aggregation.

Journal of neurochemistry

Haase C,Stieler JT,Arendt T,Holzer M

Mon Mar 01 00:00:00 EST 2004

Pseudophosphorylation of tau protein alters its ability for self-aggregation.

Journal of neurochemistry

Haase C,Stieler JT,Arendt T,Holzer M

Mon Mar 01 00:00:00 EST 2004

Formation of neurofibrillary tangles in P301l tau transgenic mice induced by Abeta 42 fibrils.

Science (New York, N.Y.)

Götz J,Chen F,van Dorpe J,Nitsch RM

44-764G was used in immunohistochemistry - paraffin section to assess induction by Abeta 42 fibrils on the formation of neurofibrillary tangles in P301I tau transgenic mice

Fri Aug 24 00:00:00 EDT 2001

Bioinformatics

Protein Aliases: FLJ31424; FTDP17; G protein beta1/gamma2 subunit-interacting factor 1; map tau; MGC138549; Microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; microtubules; Neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; Paired helical filament-tau; PHF-tau; PHFtau; protein phosphatase 1, regulatory subunit 103; Tau-4; Tau5

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Gene Aliases: DDPAC; FTDP-17; MAPT; MAPTL; MSTD; MTBT1; MTBT2; PPND; PPP1R103; TAU

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UniProt ID: (Human) P10636

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Entrez Gene ID: (Human) 4137

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Function(s)

structural constituent of cytoskeleton protein binding microtubule binding SH3 domain binding enzyme binding protein kinase binding protein complex binding apolipoprotein binding protein phosphatase 2A binding lipoprotein particle binding

Process(es)

microtubule cytoskeleton organization neuron migration brain development female pregnancy response to nutrient adult walking behavior axo-dendritic transport intrinsic apoptotic signaling pathway in response to oxidative stress response to organic substance regulation of autophagy regulation of microtubule polymerization positive regulation of microtubule polymerization negative regulation of intracellular transport positive regulation of axon extension mitochondrion transport along microtubule axon extension generation of neurons regulation of microtubule-based movement

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Phospho-Tau (Ser422) Polyclonal Antibody

Phospho-Tau (Ser422) Antibody (44-764G) in WB

Product Details

44-764G

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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