Invitrogen

Phospho-Tau (Thr212) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

18 Published Figures

32 References

View all (188) Tau antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Phospho-Tau (Thr212) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-Tau (Thr212) Antibody (44-740G) in ICC/IF

Immunofluorescent analysis of Phospho-Tau pThr212 Antibody was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Tau pThr212 Antibody (Product # 44-740G) at 1µg/mL in 1% BSA and incubated for 3 hours at ro... View More

Phospho-Tau (Thr212) Antibody in Western Blot (WB)

Phospho-Tau (Thr212) Antibody in Immunohistochemistry (IHC)

Phospho-Tau (Thr212) Antibody in ELISA (ELISA)

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Product Details

44-740G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 24 publications 24 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1 µg/mL

View 1 publication 1 publication

ELISA (ELISA)

View 2 publications 2 publications

Dot blot (DB)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Baboon, Bovine, Dog, Human, Mouse, Non-human primate, Rat, Sheep, Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Tau that contains threonine 212. The sequence is conserved in many species including mouse, rat, rhesus monkey, baboon, cow, and goat

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533739

Product Specific Information

Purified from rabbit serum by sequential epitope-specific chromatography, this product contains enough material for 10 mini-blots. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Tau protein. The final product is generated by affinity chromatography using a Tau-derived peptide that is phosphorylated at threonine 212 in isoform Tau (441). For western analysis the positive control used was recombinant human Tau +/- GSK-3beta.

Target Information

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Tau antibody 77G7 targeting microtubule binding domain suppresses proteopathic tau to seed tau aggregation.

CNS neuroscience & therapeutics

Li L,Miao J,Chu D,Jin N,Tung YC,Dai CL,Hu W,Gong CX,Iqbal K,Liu F

44-740G was used in Immunohistochemistry-immunofluorescence to indicate that 77G7 can effectively suppress the seeding activity of AD O-tau and thus could be developed as a potential immunotherapeutic drug to inhibit the propagation of tau pathology in AD and related tauopathies.

Thu Dec 01 00:00:00 EST 2022

Tau antibody 77G7 targeting microtubule binding domain suppresses proteopathic tau to seed tau aggregation.

CNS neuroscience & therapeutics

Li L,Miao J,Chu D,Jin N,Tung YC,Dai CL,Hu W,Gong CX,Iqbal K,Liu F

Thu Dec 01 00:00:00 EST 2022

Impact of increased APP gene dose in Down syndrome and the Dp16 mouse model.

Alzheimer's & dementia : the journal of the Alzheimer's Association

Sawa M,Overk C,Becker A,Derse D,Albay R,Weldy K,Salehi A,Beach TG,Doran E,Head E,Yu YE,Mobley WC

44-740G was used in Western Blotting to investigate if and which amyloid precursor protein (APP) products contribute to Alzheimer's disease (AD).

Wed Jun 01 00:00:00 EDT 2022

Dephosphorylation Passivates the Seeding Activity of Oligomeric Tau Derived From Alzheimer's Brain.

Frontiers in molecular neuroscience

Wu R,Li L,Shi R,Zhou Y,Jin N,Gu J,Tung YC,Liu F,Chu D

Published figure using Phospho-Tau (Thr212) polyclonal antibody (Product # 44-740G) in Western Blot

Tue Jun 01 00:00:00 EDT 2021

Protein phosphatase PPM1B inhibits DYRK1A kinase through dephosphorylation of pS258 and reduces toxic tau aggregation.

The Journal of biological chemistry

Lee YH,Im E,Hyun M,Park J,Chung KC

44-740G was used in Western Blotting to investigate DYRK1A interactions in Down syndrome.

Tue Aug 24 00:00:00 EDT 2021

Protein phosphatase PPM1B inhibits DYRK1A kinase through dephosphorylation of pS258 and reduces toxic tau aggregation.

The Journal of biological chemistry

Lee YH,Im E,Hyun M,Park J,Chung KC

44-740G was used in Western Blotting to investigate DYRK1A interactions in Down syndrome.

Tue Aug 24 00:00:00 EDT 2021

Proline Hydroxylation Primes Protein Kinases for Autophosphorylation and Activation.

Molecular cell

Lee SB,Ko A,Oh YT,Shi P,D'Angelo F,Frangaj B,Koller A,Chen EI,Cardozo T,Iavarone A,Lasorella A

44-740G was used in Western Blot to show that a highly conserved proline in the CMGC insert of the DYRK1 kinase domain is hydroxylated by PHD1 prolyl hydroxylase, and this event precedes tyrosine autophosphorylation.

Thu Aug 06 00:00:00 EDT 2020

The green tea polyphenol epigallocatechin-3-gallate (EGCG) restores CDKL5-dependent synaptic defects in vitro and in vivo.

Neurobiology of disease

Trovò L,Fuchs C,De Rosa R,Barbiero I,Tramarin M,Ciani E,Rusconi L,Kilstrup-Nielsen C

44-740G was used in Western Blotting to demonstrate that EGCG treatment efficiently restores defects in dendritic and synaptic development of Cdkl5-KO hippocampal neurons.

Fri May 01 00:00:00 EDT 2020

Truncation of Tau selectively facilitates its pathological activities.

The Journal of biological chemistry

Gu J,Xu W,Jin N,Li L,Zhou Y,Chu D,Gong CX,Iqbal K,Liu F

44-740G was used in Western Blotting to suggest that the first 150 aa and the last 50 aa protect Tau from pathological characteristics and that their deletions facilitate pathological activities.

Fri Oct 02 00:00:00 EDT 2020

Pathological Tau From Alzheimer's Brain Induces Site-Specific Hyperphosphorylation and SDS- and Reducing Agent-Resistant Aggregation of Tau in vivo.

Frontiers in aging neuroscience

Miao J,Shi R,Li L,Chen F,Zhou Y,Tung YC,Hu W,Gong CX,Iqbal K,Liu F

44-740G was used in Western Blotting to speculate that AD P-tau seeds hyperphosphorylated tau to form aggregates, which resist to the dephosphorylation by PP2A, resulting in hyperphosphorylation and pathology of tau.

Thu Oct 01 00:00:00 EDT 2020

Relevance of Phosphorylation and Truncation of Tau to the Etiopathogenesis of Alzheimer's Disease.

Frontiers in aging neuroscience

Zhou Y,Shi J,Chu D,Hu W,Guan Z,Gong CX,Iqbal K,Liu F

Published figure using Phospho-Tau (Thr212) polyclonal antibody (Product # 44-740G) in Western Blot

Sat Apr 09 00:00:00 EDT 2022

Involvement of Activation of Asparaginyl Endopeptidase in Tau Hyperphosphorylation in Repetitive Mild Traumatic Brain Injury.

Journal of Alzheimer's disease : JAD

Hu W,Tung YC,Zhang Y,Liu F,Iqbal K

Published figure using Phospho-Tau (Thr212) polyclonal antibody (Product # 44-740G) in Western Blot

Mon Jul 15 00:00:00 EDT 2019

Involvement of Activation of Asparaginyl Endopeptidase in Tau Hyperphosphorylation in Repetitive Mild Traumatic Brain Injury.

Journal of Alzheimer's disease : JAD

Hu W,Tung YC,Zhang Y,Liu F,Iqbal K

Published figure using Phospho-Tau (Thr212) polyclonal antibody (Product # 44-740G) in Western Blot

Mon Jul 15 00:00:00 EDT 2019

Novel Scaffolds for Dual Specificity Tyrosine-Phosphorylation-Regulated Kinase (DYRK1A) Inhibitors.

Journal of medicinal chemistry

Czarna A,Wang J,Zelencova D,Liu Y,Deng X,Choi HG,Zhang T,Zhou W,Chang JW,Kildalsen H,Seternes OM,Gray NS,Engh RA,Rothweiler U

44-740G was used in Western Blotting to report a variety of scaffolds not generally known for DYRK1A inhibition, demonstrating their effects in in vitro assays and also in cell cultures.

Thu Sep 13 00:00:00 EDT 2018

Expression of Tau Pathology-Related Proteins in Different Brain Regions: A Molecular Basis of Tau Pathogenesis.

Frontiers in aging neuroscience

Hu W,Wu F,Zhang Y,Gong CX,Iqbal K,Liu F

44-740G was used in Dot blot to provide the regional expression profiles of tau and tau pathology-related proteins in the brain.

Thu Oct 01 00:00:00 EDT 2020

Systematic diversification of benzylidene heterocycles yields novel inhibitor scaffolds selective for Dyrk1A, Clk1 and CK2.

European journal of medicinal chemistry

Mariano M,Hartmann RW,Engel M

44740G was used in western blot to identify and characterize inhibitors of Dyrk1A and Clk1

Wed Apr 13 00:00:00 EDT 2016

A chemical with proven clinical safety rescues Down-syndrome-related phenotypes in through DYRK1A inhibition.

Disease models & mechanisms

Kim H,Lee KS,Kim AK,Choi M,Choi K,Kang M,Chi SW,Lee MS,Lee JS,Lee SY,Song WJ,Yu K,Cho S

44-740G was used in western blot identify and characterize a DYRK1A inhibitor

Mon Aug 01 00:00:00 EDT 2016

Human Truncated Tau Induces Mature Neurofibrillary Pathology in a Mouse Model of Human Tauopathy.

Journal of Alzheimer's disease : JAD

Zimova I,Brezovakova V,Hromadka T,Weisova P,Cubinkova V,Valachova B,Filipcik P,Jadhav S,Smolek T,Novak M,Zilka N

44740G was used in western blot to describe the molecular and behavioral features of R3m/4 transgenic mice expressing human non-mutated truncated tau protein and assess these mice as a model for Alzheimer's disease

Tue Sep 06 00:00:00 EDT 2016

Tau hyperphosphorylation in synaptosomes and neuroinflammation are associated with canine cognitive impairment.

The Journal of comparative neurology