Invitrogen

Phospho-Tau (Thr231) Polyclonal Antibody

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This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

10 Published Figures

13 References

View all (188) Tau antibodies

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Datasheet

Protocols

Questions & Answers

Phospho-Tau (Thr231) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-Tau (Thr231) Antibody (44-746G) in ICC/IF

Immunofluorescence analysis of Phospho-Tau pThr231 Antibody was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Tau pThr231 Antibody (44746g) at 1µg/mL in 1% BSA and incubated for 3 hours at room tempera... View More

Phospho-Tau (Thr231) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-Tau (Thr231) Antibody in Western Blot (WB)

Phospho-Tau (Thr231) Antibody in Immunocytochemistry, Immunohistochemistry (Paraffin) (ICC/IF, IHC (P))

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Product Details

44-746G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1 µg/mL

View 2 publications 2 publications

ELISA (ELISA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat, Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Tau that contains threonine 231. The sequence is conserved in mouse and rat.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533742

Target Information

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

GSKIP-Mediated Anchoring Increases Phosphorylation of Tau by PKA but Not by GSK3beta via cAMP/PKA/GSKIP/GSK3/Tau Axis Signaling in Cerebrospinal Fluid and iPS Cells in Alzheimer Disease.

Journal of clinical medicine

Ko HJ,Chiou SJ,Wong YH,Wang YH,Lai Y,Chou CH,Wang C,Loh JK,Lieu AS,Cheng JT,Lin YT,Lu PJ,Fann MJ,Huang CF,Hong YR

Published figure using Phospho-Tau (Thr231) polyclonal antibody (Product # 44-746G) in Western Blot

Mon Oct 21 00:00:00 EDT 2019

The neuritic plaque facilitates pathological conversion of tau in an Alzheimer's disease mouse model.

Nature communications

Li T,Braunstein KE,Zhang J,Lau A,Sibener L,Deeble C,Wong PC

Published figure using Phospho-Tau (Thr231) polyclonal antibody (Product # 44-746G) in Immunofluorescence

Mon Jul 04 00:00:00 EDT 2016

The neuritic plaque facilitates pathological conversion of tau in an Alzheimer's disease mouse model.

Nature communications

Li T,Braunstein KE,Zhang J,Lau A,Sibener L,Deeble C,Wong PC

Published figure using Phospho-Tau (Thr231) polyclonal antibody (Product # 44-746G) in Immunofluorescence

Mon Jul 04 00:00:00 EDT 2016

Activation of Cdk5/p25 and tau phosphorylation following chronic brain hypoperfusion in rats involves microRNA-195 down-regulation.

Journal of neurochemistry

Sun LH,Ban T,Liu CD,Chen QX,Wang X,Yan ML,Hu XL,Su XL,Bao YN,Sun LL,Zhao LJ,Pei SC,Jiang XM,Zong DK,Ai J

44-746G was used in western blot to investigate the involvement of microRNA-195 in the effect of chronic brain hypoperfusion on rodent Cdk5/p25 and tau phosphorylation

Tue Sep 01 00:00:00 EDT 2015

Terminal hypothermic Tau.P301L mice have increased Tau phosphorylation independently of glycogen synthase kinase 3α/β.

The European journal of neuroscience

Maurin H,Lechat B,Borghgraef P,Devijver H,Jaworski T,Van Leuven F

44-746G was used in western blot to study the lack of involvementof GSK3-alpha/beta in the elevated tau phosphorylation observed in Tau.P30L hypothermic mice

Tue Jul 01 00:00:00 EDT 2014

β-amyloid induces a dying-back process and remote trans-synaptic alterations in a microfluidic-based reconstructed neuronal network.

Acta neuropathologica communications

Deleglise B,Magnifico S,Duplus E,Vaur P,Soubeyre V,Belle M,Vignes M,Viovy JL,Jacotot E,Peyrin JM,Brugg B

44-746G was used in immunocytochemistry to study microfluidic-based reconstructed neuronal network and beta-amyloic inducing a dying-back process and remote trans-synaptic alterations

Thu Sep 25 00:00:00 EDT 2014

Age-dependent effects of A53T alpha-synuclein on behavior and dopaminergic function.

PloS one

Oaks AW,Frankfurt M,Finkelstein DI,Sidhu A

44-746G was used in western blot to use a mouse model to examine motor activity, anxiety-like, and depressive-like behaviors both before and after the onset of Parkinson's disease

Mon Sep 16 00:00:00 EDT 2013

Asp664 cleavage of amyloid precursor protein induces tau phosphorylation by decreasing protein phosphatase 2A activity.

Journal of neurochemistry

Park SS,Jung HJ,Kim YJ,Park TK,Kim C,Choi H,Mook-Jung IH,Koo EH,Park SA

44-746G was used in western blot to investigate the effect of caspase cleavage of APP on tau phosphorylation in relation to Aβ.

Sat Dec 01 00:00:00 EST 2012

Deletion of tau attenuates heat shock-induced injury in cultured cortical neurons.

Journal of neuroscience research

Miao Y,Chen J,Zhang Q,Sun A

Fri Jan 01 00:00:00 EST 2010

Developmental regulation of tau phosphorylation, tau kinases, and tau phosphatases.

Journal of neurochemistry

Yu Y,Run X,Liang Z,Li Y,Liu F,Liu Y,Iqbal K,Grundke-Iqbal I,Gong CX

Sun Mar 01 00:00:00 EST 2009

Developmental regulation of tau phosphorylation, tau kinases, and tau phosphatases.

Journal of neurochemistry

Yu Y,Run X,Liang Z,Li Y,Liu F,Liu Y,Iqbal K,Grundke-Iqbal I,Gong CX

Sun Mar 01 00:00:00 EST 2009

Increased tau phosphorylation and cleavage in mouse models of type 1 and type 2 diabetes.

Endocrinology

Kim B,Backus C,Oh S,Hayes JM,Feldman EL

44-746G was used in immunohistochemistry and western blot to assess tau modification in type 1 and type 2 mouse models of diabetes

Tue Dec 01 00:00:00 EST 2009

A putative role for cell cycle-related proteins in microtubule-based neuroplasticity.

The European journal of neuroscience

Schmetsdorf S,Arnold E,Holzer M,Arendt T,Gärtner U

Sun Mar 01 00:00:00 EST 2009

Pseudophosphorylation of tau protein alters its ability for self-aggregation.

Journal of neurochemistry

Haase C,Stieler JT,Arendt T,Holzer M

Mon Mar 01 00:00:00 EST 2004

Pseudophosphorylation of tau protein alters its ability for self-aggregation.

Journal of neurochemistry

Haase C,Stieler JT,Arendt T,Holzer M

Mon Mar 01 00:00:00 EST 2004

Bioinformatics

Protein Aliases: FLJ31424; FTDP17; G protein beta1/gamma2 subunit-interacting factor 1; map tau; MGC138549; Microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; microtubules; Neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; Paired helical filament-tau; PHF-tau; PHFtau; protein phosphatase 1, regulatory subunit 103; Tau-4; Tau5

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Gene Aliases: AI413597; AW045860; DDPAC; FTDP-17; MAPT; MAPTL; MSTD; Mtapt; MTBT1; MTBT2; PPND; PPP1R103; TAU

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UniProt ID: (Human) P10636, (Mouse) P10637

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Entrez Gene ID: (Human) 4137, (Mouse) 17762

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Function(s)

structural constituent of cytoskeleton protein binding microtubule binding SH3 domain binding enzyme binding protein kinase binding protein complex binding apolipoprotein binding protein phosphatase 2A binding lipoprotein particle binding tubulin binding protein domain specific binding

Process(es)

microtubule cytoskeleton organization neuron migration brain development female pregnancy response to nutrient adult walking behavior axo-dendritic transport intrinsic apoptotic signaling pathway in response to oxidative stress response to organic substance regulation of autophagy regulation of microtubule polymerization positive regulation of microtubule polymerization negative regulation of intracellular transport positive regulation of axon extension mitochondrion transport along microtubule axon extension generation of neurons regulation of microtubule-based movement apoptotic process axonogenesis

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Phospho-Tau (Thr231) Polyclonal Antibody

Phospho-Tau (Thr231) Antibody (44-746G) in ICC/IF

Product Details

44-746G

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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