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Invitrogen
Phospho-VEGF Receptor 2 (Tyr1054, Tyr1059) Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 9
Phospho-VEGF Receptor 2 (Tyr1054, Tyr1059) Antibody (44-1047G) in ICC/IF
Immunofluorescence analysis of Phospho-VEGF Receptor 2 pTyr1054/pTyr1059 was done on 90% confluent log phase HUVEC cell treated with 100 ng of VEGF for 30 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-VEGF Receptor 2 pTyr1054/pTyr1059 Rabb... View More
Product Details
44-1047G
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human VEGFR2 that contains tyrosines 1054 and 1059. The sequence is conserved among multiple species including mouse and rat.
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533557
Product Specific Information
On western blotting this antibody detects a protein with the apparent MW of 150 kDa. Positive controls used Porcine aortic endothelial (PAE) cells transfected with a chimeric receptor consisting of the extracellular domain of the CSF-1 receptor coupled to the transmembrane and cytoplasmic domains of the mouse VEGFR2, or 293T cells transfected with full length VEGFR2 and stimulated with 10 ng/mL VEGF for 5 minutes.
VEGFR3 has not been tested, but is expected to react with this antibody.
Target Information
KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase. It binds vascular endothelial growth factor with high affinity in vitro, and is expressed early in development by endothelial cell precursors. FLK1 (VEGF Receptor 2) encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of FLK1 (VEGF Receptor 2) are implicated in infantile capillary hemangiomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD309; CD309 antigen; Fetal liver kinase 1; fetal liver kinase-1; FLK-1; KDR; Kinase insert domain receptor; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase NYK; Protein-tyrosine kinase receptor flk-1; sCD309; soluble CD309; soluble vascular endothelial growth factor receptor 2; soluble VEGFR 2; soluble VEGFR2; tyrosine kinase growth factor receptor; Vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor- 2; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; VEGF R2; VEGF receptor-2; VEGFR-2
Gene Aliases: 6130401C07; CD309; Flk-1; FLK1; KDR; Krd-1; Ly73; sVEGFR-2; VEGFR; VEGFR-2; VEGFR2
UniProt ID: (Human) P35968, (Mouse) P35918
Entrez Gene ID: (Human) 3791, (Mouse) 16542
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
receptor signaling protein tyrosine kinase activity
vascular endothelial growth factor-activated receptor activity
integrin binding
protein binding
ATP binding
growth factor binding
Hsp90 protein binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
vascular endothelial growth factor binding
transmembrane signal receptor
angiogenesis
vasculogenesis
positive regulation of protein phosphorylation
positive regulation of endothelial cell proliferation
cell migration involved in sprouting angiogenesis
endothelium development
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of cell proliferation
regulation of cell shape
positive regulation of endothelial cell migration
positive regulation of phosphatidylinositol 3-kinase signaling
viral process
positive regulation of macroautophagy
peptidyl-tyrosine phosphorylation
signal transduction by protein phosphorylation
extracellular matrix organization
positive regulation of cell migration
embryonic hemopoiesis
calcium-mediated signaling using intracellular calcium source
cellular response to vascular endothelial growth factor stimulus
positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway
peptidyl-tyrosine autophosphorylation
negative regulation of apoptotic process
positive regulation of MAPK cascade
positive regulation of angiogenesis
protein autophosphorylation
vascular endothelial growth factor receptor signaling pathway
positive regulation of positive chemotaxis
positive regulation of nitric-oxide synthase biosynthetic process
positive regulation of focal adhesion assembly
positive regulation of mitochondrial depolarization
positive regulation of ERK1 and ERK2 cascade
positive regulation of mitochondrial fission
negative regulation of endothelial cell apoptotic process
positive regulation of vasculogenesis
ovarian follicle development
response to hypoxia
regulation of endothelial cell proliferation
lymph vessel development
positive regulation of mesenchymal cell proliferation
endocardium development
endochondral bone growth
protein phosphorylation
positive regulation of cytosolic calcium ion concentration
multicellular organism development
male gonad development
phosphorylation
cell migration
hemopoiesis
cell differentiation
lung development
positive regulation of BMP signaling pathway
positive regulation of vascular endothelial growth factor receptor signaling pathway
positive regulation of TOR signaling
vascular endothelial growth factor signaling pathway
response to drug
surfactant homeostasis
negative regulation of neuron apoptotic process
cell fate commitment
endothelial cell differentiation
regulation of endothelial cell differentiation
positive regulation of vasodilation
positive regulation of long-term neuronal synaptic plasticity
lung alveolus development
cell maturation
branching morphogenesis of an epithelial tube
neuron projection morphogenesis
positive regulation of epithelial cell proliferation
positive regulation of calcium-mediated signaling
calcium ion homeostasis
blood vessel endothelial cell differentiation
regulation of hematopoietic progenitor cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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