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Invitrogen
Phospho-YAP1 (Ser127) Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
Phospho-YAP1 (Ser127) Antibody (PA5-17481) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MDA-MB-231 treated with Forskolin (10 µM for 60 min) (Lane 2), PC-3 (Lane 3), PC-3 treated with Forskolin (10 µM for 60 min) and U-87 MG (Lane 5). The blot was probed with Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody (Product # PA5-17481, 1:1,000 dilution) and detected by chemiluminescence using Goat anti-Rab... View More
Product Details
PA5-17481
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding pSer127 of human YAP
Conjugate
Form
Liquid
Concentration
59.2 µg/mL
Purification
Antigen affinity chromatography, Protein A
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10981511
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
YAP (Yes-Associated Protein) is a founding member of the WW domain family of proteins, transcriptional co-activator and oncogene. Its gene is transcriptionally activated in squamous cell carcinoma cells. YAP can act both as a coactivator and corepressor and is the critical downstream regulatory target in the Hippo signaling pathway. The Hippo signaling pathway plays a pivotal role in organ size control and tumour suppression by restricting proliferation and promoting apoptosis. Also plays key roles in tissue tension, 3D tissue shape, cell proliferation, cell death, and cell migration. Mutations in the IRF5 gene results in coloboma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 65 kDa Yes-associated protein; Protein yorkie homolog; Transcriptional coactivator YAP1; Yes-associated protein 1; yes-associated protein 2; Yes-associated protein YAP65 homolog; yes-associated protein, 65 kDa; yorkie homolog
Gene Aliases: AI325207; COB1; YAP; YAP1; YAP2; YAP65; YKI; Yorkie
UniProt ID: (Human) P46937, (Mouse) P46938
Entrez Gene ID: (Human) 10413, (Rat) 363014, (Mouse) 22601
RNA polymerase II core promoter sequence-specific DNA binding
transcription factor activity, RNA polymerase II transcription factor binding
chromatin binding
transcription coactivator activity
transcription corepressor activity
protein binding
protein C-terminus binding
transcription regulatory region DNA binding
proline-rich region binding
transcription cofactor
cell morphogenesis
vasculogenesis
embryonic heart tube morphogenesis
transcription initiation from RNA polymerase II promoter
cellular response to DNA damage stimulus
cell proliferation
positive regulation of cell proliferation
regulation of keratinocyte proliferation
keratinocyte differentiation
negative regulation of epithelial cell differentiation
notochord development
somatic stem cell population maintenance
hippo signaling
positive regulation of transcription from RNA polymerase II promoter
positive regulation of organ growth
paraxial mesoderm development
lateral mesoderm development
regulation of neurogenesis
contact inhibition
bud elongation involved in lung branching
lung epithelial cell differentiation
cellular response to retinoic acid
cellular response to gamma radiation
regulation of stem cell proliferation
regulation of metanephric nephron tubule epithelial cell differentiation
positive regulation of canonical Wnt signaling pathway
positive regulation of stem cell population maintenance
negative regulation of nucleic acid-templated transcription
negative regulation of stem cell differentiation
negative regulation of extrinsic apoptotic signaling pathway
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
DNA damage induced protein phosphorylation
regulation of cell proliferation
regulation of canonical Wnt signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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