Invitrogen

Phospho-c-Met (Tyr1230, Tyr1234, Tyr1235) Polyclonal Antibody

15 Published Figures

38 References

View all (88) c-Met antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (15)

Phospho-c-Met (Tyr1230, Tyr1234, Tyr1235) Antibody in Western Blot (WB)

FIGURE: 1 / 17

Phospho-c-Met (Tyr1230, Tyr1234, Tyr1235) Antibody (44-888G) in WB

Peptide Competition: Extracts prepared from 293T cells left unstimulated (1) or stimulated with HGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C

Phospho-c-Met (Tyr1230, Tyr1234, Tyr1235) Antibody in peptide-ELISA (pep-ELISA)

Phospho-c-Met (Tyr1230, Tyr1234, Tyr1235) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Phospho-c-Met (Tyr1230, Tyr1234, Tyr1235) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-c-Met (Tyr1230, Tyr1234, Tyr1235) Antibody in Immunohistochemistry (IHC)

Phospho-c-Met (Tyr1230, Tyr1234, Tyr1235) Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

44-888G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 19 publications 19 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

peptide-ELISA (pep-ELISA)

Assay-dependent

Miscellaneous PubMed (Misc)

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Dog, Hamster, Human, Mouse, Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human c-Met that contains tyrosines 1230, 1234 and 1235. The sequence is conserved in mouse and rat.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533787

Target Information

MET (cMET) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Tyrosine Kinase c-MET as Therapeutic Target for Radiosensitization of Head and Neck Squamous Cell Carcinomas.

Cancers

Lüttich L,Besso MJ,Heiden S,Koi L,Baumann M,Krause M,Dubrovska A,Linge A,Kurth I,Peitzsch C

44-888G was used in Western Blotting to validate the prognostic value of c-MET in patients with head and neck squamous cell carcinoma (HNSCC) treated with radio(chemo)therapy using the Cancer Genome Atlas database and find an association of increased MET gene expression and protein phosphorylation with reduced disease-specific and progression-free survival.

Wed Apr 14 00:00:00 EDT 2021

Tyrosine Kinase c-MET as Therapeutic Target for Radiosensitization of Head and Neck Squamous Cell Carcinomas.

Cancers

Lüttich L,Besso MJ,Heiden S,Koi L,Baumann M,Krause M,Dubrovska A,Linge A,Kurth I,Peitzsch C

Wed Apr 14 00:00:00 EDT 2021

Infiltrating Mast Cell-Mediated Stimulation of Estrogen Receptor Activity in Breast Cancer Cells Promotes the Luminal Phenotype.

Cancer research

Majorini MT,Cancila V,Rigoni A,Botti L,Dugo M,Triulzi T,De Cecco L,Fontanella E,Jachetti E,Tagliabue E,Chiodoni C,Tripodo C,Colombo MP,Lecis D

44-888G was used in Western Blot to support the notion that MCs influence the phenotype of breast cancer cells by stimulating a luminal phenotype and ultimately modifying the outcome of the disease.

Mon Jun 01 00:00:00 EDT 2020

Efficacy and Effect of Cabozantinib on Bone Metastases in Treatment-naive Castration-resistant Prostate Cancer.

Clinical genitourinary cancer

Smith DC,Daignault-Newton S,Grivas P,Reichert ZR,Hussain M,Cooney KA,Caram M,Alva A,Jacobson J,Yablon C,Mehra R,Escara-Wilke J,Shelley G,Keller ET

44-888G was used in Immunohistochemistry to evaluate the efficacy and changes in bone lesions in men with metastatic castration-resistant prostate cancer (mCRPC) treated with cabozantinib.

Sat Aug 01 00:00:00 EDT 2020

MiR-16 regulates the pro-tumorigenic potential of lung fibroblasts through the inhibition of HGF production in an FGFR-1- and MEK1-dependent manner.

Journal of hematology & oncology

Andriani F,Majorini MT,Mano M,Landoni E,Miceli R,Facchinetti F,Mensah M,Fontanella E,Dugo M,Giacca M,Pastorino U,Sozzi G,Delia D,Roz L,Lecis D

44-888G was used in Western Blotting to uncover a central role for miR-16 in regulating HGF production by lung fibroblasts, thus affecting their pro-tumorigenic potential.

Tue Mar 20 00:00:00 EDT 2018

Cabozantinib Eradicates Advanced Murine Prostate Cancer by Activating Antitumor Innate Immunity.

Cancer discovery

Patnaik A,Swanson KD,Csizmadia E,Solanki A,Landon-Brace N,Gehring MP,Helenius K,Olson BM,Pyzer AR,Wang LC,Elemento O,Novak J,Thornley TB,Asara JM,Montaser L,Timmons JJ,Morgan TM,Wang Y,Levantini E,Clohessy JG,Kelly K,Pandolfi PP,Rosenblatt JM,Avigan DE,Ye H,Karp JM,Signoretti S,Balk SP,Cantley LC

44-888G was used in Immunohistochemistry to investigate the impact of cabozantinib on the tumour microenvironment.

Sat Jul 01 00:00:00 EDT 2017

Riparsaponin isolated from Homonoia riparia Lour induces apoptosis of oral cancer cells.

Oncology letters

Li T,Wang L

44-888G was used in Western Blotting to investigate the antitumour effect of riparsaponin on human oral carcinoma cells, and its potential underlying molecular mechanism.

Fri Dec 01 00:00:00 EST 2017

Zebrafish cerebrospinal fluid mediates cell survival through a retinoid signaling pathway.

Developmental neurobiology

Chang JT,Lehtinen MK,Sive H

44-888G was used in immunohistochemistry to examine the contribution of cerebrospinal fluid during embryonic development.

Fri Jan 01 00:00:00 EST 2016

Ovarian cancer ascites enhance the migration of patient-derived peritoneal mesothelial cells via cMet pathway through HGF-dependent and -independent mechanisms.

International journal of cancer

Matte I,Lane D,Laplante C,Garde-Granger P,Rancourt C,Piché A

Published figure using Phospho-c-Met (Tyr1230, Tyr1234, Tyr1235) polyclonal antibody (Product # 44-888G) in Western Blot

Wed Jul 15 00:00:00 EDT 2015

Polycystin-1 and polycystin-2 are involved in the acquisition of aggressive phenotypes in colorectal cancer.

International journal of cancer

Gargalionis AN,Korkolopoulou P,Farmaki E,Piperi C,Dalagiorgou G,Adamopoulos C,Levidou G,Saetta A,Fragkou P,Tsioli P,Kiaris H,Zizi-Serbetzoglou A,Karavokyros I,Papavassiliou KA,Tsavaris N,Patsouris E,Basdra EK,Papavassiliou AG

44-888G was used in immunohistochemistry to assess if PC1 and PC2 facilitate cancer aggressiveness.

Wed Apr 01 00:00:00 EDT 2015

Combination strategy targeting VEGF and HGF/c-met in human renal cell carcinoma models.

Molecular cancer therapeutics

Ciamporcero E,Miles KM,Adelaiye R,Ramakrishnan S,Shen L,Ku S,Pizzimenti S,Sennino B,Barrera G,Pili R

Published figure using Phospho-c-Met (Tyr1230, Tyr1234, Tyr1235) polyclonal antibody (Product # 44-888G) in Immunofluorescence

Thu Jan 01 00:00:00 EST 2015

Metastasis initiating cells in primary prostate cancer tissues from transurethral resection of the prostate (TURP) predicts castration-resistant progression and survival of prostate cancer patients.

The Prostate

Li Q,Li Q,Nuccio J,Liu C,Duan P,Wang R,Jones LW,Chung LW,Zhau HE

44-888G was used in immunohistochemistry to examine the contribution of metastasis-initiating cells to prostate cancer.

Tue Sep 01 00:00:00 EDT 2015

Oncotarget

Kinose Y,Sawada K,Nakamura K,Sawada I,Toda A,Nakatsuka E,Hashimoto K,Mabuchi S,Takahashi K,Kurachi H,Lengyel E,Kimura T

Published figure using Phospho-c-Met (Tyr1230, Tyr1234, Tyr1235) polyclonal antibody (Product # 44-888G) in Western Blot

Sun May 10 00:00:00 EDT 2015

The Ser/Thr kinase MAP4K4 drives c-Met-induced motility and invasiveness in a cell-based model of SHH medulloblastoma.

SpringerPlus

Santhana Kumar K,Tripolitsioti D,Ma M,Grählert J,Egli KB,Fiaschetti G,Shalaby T,Grotzer MA,Baumgartner M

44-888G was used in immunocytochemistry and western blot to assess the contribution of c-Met signaling to dissemination of medulloblastoma

Tue Jan 27 00:00:00 EST 2015

Spatially restricted Hedgehog signalling regulates HGF-induced branching of the adult prostate.

Nature cell biology

Lim A,Shin K,Zhao C,Kawano S,Beachy PA

44-888G was used in western blot to investigate the mechanisms underlying the specification of branch location

Mon Dec 01 00:00:00 EST 2014

Genetic suppression of inflammation blocks the tumor-promoting effects of TGF-β in gastric tissue.

Cancer research

Ota M,Horiguchi M,Fang V,Shibahara K,Kadota K,Loomis C,Cammer M,Rifkin DB

44-888G was used in immunohistochemistry to use transgenic mice to determine the contribution of TGF-β1 to lymphocyte-mediated inflammation in gastrointestinal tumorigenesis.

Thu May 01 00:00:00 EDT 2014

Inhibition of tumor growth and metastasis in non-small cell lung cancer by LY2801653, an inhibitor of several oncokinases, including MET.

Clinical cancer research : an official journal of the American Association for Cancer Research

Wu W,Bi C,Credille KM,Manro JR,Peek VL,Donoho GP,Yan L,Wijsman JA,Yan SB,Walgren RA

44-888G was used in western blot to discuss the use of LY280653 in both preclinical in vitro and in vivo non-small cell lung cancer models.

Tue Oct 15 00:00:00 EDT 2013

In vitro and in vivo activity of cabozantinib (XL184), an inhibitor of RET, MET, and VEGFR2, in a model of medullary thyroid cancer.

Thyroid : official journal of the American Thyroid Association

Bentzien F,Zuzow M,Heald N,Gibson A,Shi Y,Goon L,Yu P,Engst S,Zhang W,Huang D,Zhao L,Vysotskaia V,Chu F,Bautista R,Cancilla B,Lamb P,Joly AH,Yakes FM

Published figure using Phospho-c-Met (Tyr1230, Tyr1234, Tyr1235) polyclonal antibody (Product # 44-888G) in Immunohistochemistry

Sun Dec 01 00:00:00 EST 2013

MET expression in melanoma correlates with a lymphangiogenic phenotype.

Human molecular genetics

Swoboda A,Schanab O,Tauber S,Bilban M,Berger W,Petzelbauer P,Mikula M

44-888G was used in western blot to identify lymphangiogenic subpopulations from a melanoma of a single patient.

Wed Aug 01 00:00:00 EDT 2012

Preservations of nephrin and synaptopodin by recombinant hepatocyte growth factor in podocytes for the attenuations of foot process injury and albuminuria in nephritic mice.

Nephrology (Carlton, Vic.)

Kato T,Mizuno S,Nakamura T

44-888G was used in immunohistochemistry - paraffin section to investigate the effect of hepatocyte growth factor on nephrin and synaptopodin expression.

Tue Mar 01 00:00:00 EST 2011

MET and phosphorylated MET as potential biomarkers in lung cancer.

Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer

Tretiakova M,Salama AK,Karrison T,Ferguson MK,Husain AN,Vokes EE,Salgia R

44-888G was used in immunohistochemistry to assess expression and prognostic role of the receptor tyrosine kinase MET, phosphorylated MET, and the ligand hepatocyte growth factor in patients with lung cancer.

Thu Feb 16 00:00:00 EST 2012

Multiplexed quantum dot labeling of activated c-Met signaling in castration-resistant human prostate cancer.

PloS one

Hu P,Chu GC,Zhu G,Yang H,Luthringer D,Prins G,Habib F,Wang Y,Wang R,Chung LW,Zhau HE

44-888G was used in western blot to compare multiplexed quantum dot labeling with other techniques to identify prostate cancer.

Mon Apr 23 00:00:00 EDT 2012

Multiplexed quantum dot labeling of activated c-Met signaling in castration-resistant human prostate cancer.

PloS one

Hu P,Chu GC,Zhu G,Yang H,Luthringer D,Prins G,Habib F,Wang Y,Wang R,Chung LW,Zhau HE

44-888G was used in western blot to compare multiplexed quantum dot labeling with other techniques to identify prostate cancer.

Mon Apr 23 00:00:00 EDT 2012

Expression patterns of PAX5, c-Met, and paxillin in neuroendocrine tumors of the lung.

Archives of pathology & laboratory medicine

Song J,Li M,Tretiakova M,Salgia R,Cagle PT,Husain AN

44-888G was used in immunohistochemistry - paraffin section to examine the expression of PAX5, c-Met, and paxillin in in neuroendocrine tumors of the lung

Mon Nov 01 00:00:00 EDT 2010

Different changes in protein and phosphoprotein levels result from serum starvation of high-grade glioma and adenocarcinoma cell lines.

Journal of proteome research

Levin VA,Panchabhai SC,Shen L,Kornblau SM,Qiu Y,Baggerly KA

Fri Jan 01 00:00:00 EST 2010

Normalization of wound healing and diabetic markers in organ cultured human diabetic corneas by adenoviral delivery of c-Met gene.

Investigative ophthalmology & visual science

Saghizadeh M,Kramerov AA,Yu FS,Castro MG,Ljubimov AV

44-888G was used in Western Blotting to study the therapeutic potential of c-met overexpression in correcting molecular pathogenic traits in diabetic corneas.

Thu Apr 01 00:00:00 EDT 2010

PAX3 and SOX10 activate MET receptor expression in melanoma.

Pigment cell & melanoma research

Mascarenhas JB,Littlejohn EL,Wolsky RJ,Young KP,Nelson M,Salgia R,Lang D

Published figure using Phospho-c-Met (Tyr1230, Tyr1234, Tyr1235) polyclonal antibody (Product # 44-888G) in Immunofluorescence

Thu Apr 01 00:00:00 EDT 2010

PAX3 and SOX10 activate MET receptor expression in melanoma.

Pigment cell & melanoma research

Mascarenhas JB,Littlejohn EL,Wolsky RJ,Young KP,Nelson M,Salgia R,Lang D

Published figure using Phospho-c-Met (Tyr1230, Tyr1234, Tyr1235) polyclonal antibody (Product # 44-888G) in Immunofluorescence

Thu Apr 01 00:00:00 EDT 2010

PAX3 and SOX10 activate MET receptor expression in melanoma.

Pigment cell & melanoma research

Mascarenhas JB,Littlejohn EL,Wolsky RJ,Young KP,Nelson M,Salgia R,Lang D

Published figure using Phospho-c-Met (Tyr1230, Tyr1234, Tyr1235) polyclonal antibody (Product # 44-888G) in Immunofluorescence

Thu Apr 01 00:00:00 EDT 2010

Vascular endothelial growth factor regulates myeloid cell leukemia-1 expression through neuropilin-1-dependent activation of c-MET signaling in human prostate cancer cells.

Molecular cancer

Zhang S,Zhau HE,Osunkoya AO,Iqbal S,Yang X,Fan S,Chen Z,Wang R,Marshall FF,Chung LW,Wu D

Published figure using Phospho-c-Met (Tyr1230, Tyr1234, Tyr1235) polyclonal antibody (Product # 44-888G) in Immunohistochemistry

Tue Jan 19 00:00:00 EST 2010

Vascular endothelial growth factor regulates myeloid cell leukemia-1 expression through neuropilin-1-dependent activation of c-MET signaling in human prostate cancer cells.

Molecular cancer

Zhang S,Zhau HE,Osunkoya AO,Iqbal S,Yang X,Fan S,Chen Z,Wang R,Marshall FF,Chung LW,Wu D

Published figure using Phospho-c-Met (Tyr1230, Tyr1234, Tyr1235) polyclonal antibody (Product # 44-888G) in Immunohistochemistry

Tue Jan 19 00:00:00 EST 2010

The MET receptor tyrosine kinase is a potential novel therapeutic target for head and neck squamous cell carcinoma.

Cancer research

Seiwert TY,Jagadeeswaran R,Faoro L,Janamanchi V,Nallasura V,El Dinali M,Yala S,Kanteti R,Cohen EE,Lingen MW,Martin L,Krishnaswamy S,Klein-Szanto A,Christensen JG,Vokes EE,Salgia R

44-888G was used in western blot to study MET receptor tyrosine kinase as a potential novel therapeutic target for head and neck squamous cell carcinoma

Wed Apr 01 00:00:00 EDT 2009

Impairment of the antifibrotic effect of hepatocyte growth factor in lung fibroblasts from African Americans: possible role in systemic sclerosis.

Arthritis and rheumatism

Bogatkevich GS,Ludwicka-Bradley A,Highland KB,Hant F,Nietert PJ,Singleton CB,Feghali-Bostwick CA,Silver RM

Sun Jul 01 00:00:00 EDT 2007

Functional expression and mutations of c-Met and its therapeutic inhibition with SU11274 and small interfering RNA in non-small cell lung cancer.

Cancer research

Ma PC,Jagadeeswaran R,Jagadeesh S,Tretiakova MS,Nallasura V,Fox EA,Hansen M,Schaefer E,Naoki K,Lader A,Richards W,Sugarbaker D,Husain AN,Christensen JG,Salgia R

Tue Feb 15 00:00:00 EST 2005

Hepatocyte growth factor contributes to fracture repair by upregulating the expression of BMP receptors.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Imai Y,Terai H,Nomura-Furuwatari C,Mizuno S,Matsumoto K,Nakamura T,Takaoka K

Sat Oct 01 00:00:00 EDT 2005

Prevention of neutrophil extravasation by hepatocyte growth factor leads to attenuations of tubular apoptosis and renal dysfunction in mouse ischemic kidneys.

The American journal of pathology

Mizuno S,Nakamura T

Wed Jun 01 00:00:00 EDT 2005

A novel small molecule met inhibitor induces apoptosis in cells transformed by the oncogenic TPR-MET tyrosine kinase.

Cancer research

Sattler M,Pride YB,Ma P,Gramlich JL,Chu SC,Quinnan LA,Shirazian S,Liang C,Podar K,Christensen JG,Salgia R

Mon Sep 01 00:00:00 EDT 2003

Different susceptibility of human mesothelial cells to polyomavirus infection and malignant transformation.

Cancer research

Carbone M,Burck C,Rdzanek M,Rudzinski J,Cutrone R,Bocchetta M

Wed Oct 01 00:00:00 EDT 2003

A selective small molecule inhibitor of c-Met kinase inhibits c-Met-dependent phenotypes in vitro and exhibits cytoreductive antitumor activity in vivo.

Cancer research

Christensen JG,Schreck R,Burrows J,Kuruganti P,Chan E,Le P,Chen J,Wang X,Ruslim L,Blake R,Lipson KE,Ramphal J,Do S,Cui JJ,Cherrington JM,Mendel DB

Sat Nov 01 00:00:00 EST 2003

A selective small molecule inhibitor of c-Met kinase inhibits c-Met-dependent phenotypes in vitro and exhibits cytoreductive antitumor activity in vivo.

Cancer research

Christensen JG,Schreck R,Burrows J,Kuruganti P,Chan E,Le P,Chen J,Wang X,Ruslim L,Blake R,Lipson KE,Ramphal J,Do S,Cui JJ,Cherrington JM,Mendel DB

Sat Nov 01 00:00:00 EST 2003

A selective small molecule inhibitor of c-Met kinase inhibits c-Met-dependent phenotypes in vitro and exhibits cytoreductive antitumor activity in vivo.

Cancer research

Christensen JG,Schreck R,Burrows J,Kuruganti P,Chan E,Le P,Chen J,Wang X,Ruslim L,Blake R,Lipson KE,Ramphal J,Do S,Cui JJ,Cherrington JM,Mendel DB

Sat Nov 01 00:00:00 EST 2003

Bioinformatics

Protein Aliases: cMet; EC 2.7.10.1; Hepatocyte growth factor receptor; HGF receptor; HGF-SF receptor; HGF/SF receptor; met proto-oncogene tyrosine kinase; oncogene MET; Proto-oncogene c-Met; sc MET; Scatter factor receptor; SF receptor; soluble c met; Tyrosine-protein kinase Met

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Gene Aliases: AI838057; AUTS9; c-Met; DFNB97; HGF; HGFR; MET; Par4; RCCP2

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UniProt ID: (Human) P08581

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Entrez Gene ID: (Human) 4233, (Mouse) 17295

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Function(s)

protein tyrosine kinase activity hepatocyte growth factor-activated receptor activity protein binding ATP binding protein phosphatase binding nucleotide binding protein kinase activity transmembrane receptor protein tyrosine kinase activity beta-catenin binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein complex binding phospholipase binding phosphatidylinositol 3-kinase binding protein heterodimerization activity transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-c-Met (Tyr1230, Tyr1234, Tyr1235) Polyclonal Antibody

Phospho-c-Met (Tyr1230, Tyr1234, Tyr1235) Antibody (44-888G) in WB

Product Details

44-888G

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

peptide-ELISA (pep-ELISA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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