Rockland

Phospho-c-Met (Tyr1349, Tyr1356) Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

600-401-989

Applications
Tested Dilution
Publications

Western Blot (WB)

1 µg/mL
-

ELISA (ELISA)

1:93,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y1349 and Y1356 of human c-Met protein.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.96 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Antibody is specific for human c-Met protein phosphorylated at Y1349 and Y1356. A BLAST analysis was used to suggest cross-reactivity with c-Met from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with c-Met from other sources has not been determined.

Target Information

MET (cMET) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: c-Met; cMet; EC 2.7.10.1; Hepatocyte growth factor receptor; HGF receptor; HGF-SF receptor; HGF/SF receptor; met proto-oncogene tyrosine kinase; oncogene MET; Proto-oncogene c-Met; sc MET; Scatter factor receptor; SF receptor; soluble c met; Tyrosine-protein kinase Met

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Gene Aliases: AI838057; AUTS9; c-Met; DFNB97; HGF; HGFR; MET; Par4; RCCP2

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UniProt ID: (Human) P08581, (Rat) P97523

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Entrez Gene ID: (Human) 4233, (Rat) 24553, (Mouse) 17295

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Function(s)
protein tyrosine kinase activity hepatocyte growth factor-activated receptor activity protein binding ATP binding protein phosphatase binding protein kinase activity beta-catenin binding protein complex binding phospholipase binding phosphatidylinositol 3-kinase binding protein heterodimerization activity nucleotide binding transmembrane receptor protein tyrosine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups transmembrane signal receptor
Process(es)
endothelial cell morphogenesis signal transduction cell surface receptor signaling pathway cell proliferation negative regulation of autophagy peptidyl-tyrosine phosphorylation positive regulation of transcription from RNA polymerase II promoter hepatocyte growth factor receptor signaling pathway branching morphogenesis of an epithelial tube positive chemotaxis semaphorin-plexin signaling pathway negative regulation of hydrogen peroxide-mediated programmed cell death positive regulation of endothelial cell chemotaxis negative regulation of transcription from RNA polymerase II promoter activation of MAPK activity neuron migration liver development placenta development brain development muscle organ development skeletal muscle tissue development cell aging lactation male gonad development response to wounding response to acidic pH positive regulation of gene expression positive regulation of peptidyl-threonine phosphorylation negative regulation of peptidyl-threonine phosphorylation positive regulation of glucose transport positive regulation of neuron projection development oligodendrocyte development response to organic cyclic compound muscle cell migration myotube differentiation central nervous system neuron differentiation sperm motility adult behavior pancreas development positive regulation of peptidyl-serine phosphorylation response to drug glucose homeostasis response to peptide hormone positive regulation of DNA replication positive regulation of mitotic nuclear division protein autophosphorylation positive regulation of dendrite morphogenesis modulation of synaptic transmission myoblast proliferation excitatory postsynaptic potential regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling cellular response to inorganic substance cellular response to arsenic-containing substance cellular response to glucose stimulus cellular response to interleukin-6 cellular response to growth factor stimulus cellular response to peptide hormone stimulus protein phosphorylation transmembrane receptor protein tyrosine kinase signaling pathway phosphorylation
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